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E8TF57 (E8TF57_MESCW) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Mesci_3153 EMBL ADV12277.1
OrganismMesorhizobium ciceri bv. biserrulae (strain HAMBI 2942 / LMG 23838 / WSM1271) [Complete proteome] [HAMAP] EMBL ADV12277.1
Taxonomic identifier765698 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain102 – 302201ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding128 – 18356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2521Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E8TF57 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: A80D9A8629288A90

FASTA30833,303
        10         20         30         40         50         60 
MKNKHVAVLL GGFSSERPVS LSSGKACADE LEKEGYQVTR VDVGRDVGSV LAELKPDVAF 

        70         80         90        100        110        120 
NALHGPFGED GTIQGILEYL GIPYTHSGVL ASALAMNKEQ AKRVAKSVGI VVAESKVASR 

       130        140        150        160        170        180 
FAIQNKHPMK PPYVVKPVNE GSSFGVVIVH EGQSHPPQVL GSSEWKYGDT VMVERYIHGR 

       190        200        210        220        230        240 
ELTCAVMGDV ALGVCEIIPT GHSFYDYDSK YVAGGSKHEC PAKVSPNIYQ KIQTLALKAH 

       250        260        270        280        290        300 
QAIGCRGVSR SDFRYDDRHS ENGEIVWLEV NTQPGMTPTS LVPEIAAHAG HSFGDLLSWM 


VEDASCLR 

« Hide

References

[1]"Complete sequence of chromosome of Mesorhizobium ciceri bv. biserrulae WSM1271."
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Nandasena K., Reeve W.G., Howieson J.G., O'Hara G., Tiwari R.P., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HAMBI 2942 / LMG 23838 / WSM1271.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002447 Genomic DNA. Translation: ADV12277.1.
RefSeqYP_004142327.1. NC_014923.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV12277; ADV12277; Mesci_3153.
GeneID10118627.
KEGGmci:Mesci_3153.
PATRIC45256389. VBIMesCic160642_3647.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.

Enzyme and pathway databases

BioCycMCIC765698:GHQ5-3188-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8TF57_MESCW
AccessionPrimary (citable) accession number: E8TF57
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: February 19, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)