E8T632 (E8T632_THEA1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A HAMAP-Rule MF_00171
EC=5.4.99.12 HAMAP-Rule MF_00171

Alternative name(s):
tRNA pseudouridine(38-40) synthase HAMAP-Rule MF_00171
tRNA pseudouridylate synthase I HAMAP-Rule MF_00171
tRNA-uridine isomerase I HAMAP-Rule MF_00171

Gene names

Name:	truA HAMAP-Rule MF_00171
Ordered Locus Names:	Theam_0646 EMBL ADU96616.1

Organism

Thermovibrio ammonificans (strain DSM 15698 / JCM 12110 / HB-1) [Complete proteome] [HAMAP] EMBL ADU96616.1

Taxonomic identifier

648996 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Desulfurobacteriaceae › Thermovibrio › Thermovibrio ammonificans

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171 SAAS SAAS020094
Catalytic activity	tRNA uridine = tRNA pseudouridine. RuleBase RU003792 tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171 SAAS SAAS020094
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family. HAMAP-Rule MF_00171 RuleBase RU003792

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Molecular function	Isomerase HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	52	1	Nucleophile By similarity HAMAP-Rule MF_00171
	Binding site	110	1	Substrate By similarity HAMAP-Rule MF_00171

Sequences

	Sequence		Length	Mass (Da)
	E8T632 [UniParc]. Last modified April 5, 2011. Version 1. Checksum: 787DE70850FBB148	FASTA	254	29,564
	10 20 30 40 50 60 MRNFKLTVEY LGTNYYGWQF LPDKPTVQGE IIRCLETVLR HPVKLIGASR TDAGVHARGQ 70 80 90 100 110 120 VANFLTAKEV SCEKLLSALN GLLPPDIRVK EVEEVPLEFH ARRSARGKLY RYRLFIRDVP 130 140 150 160 170 180 SPFEFRRSWY LRRTLSVEPM REATQFLVGT HDFTTFSKSE RKREVNPIRT IDKIEITNKE 190 200 210 220 230 240 WEEGVLVELL FTGRSFLRHM VRVIVATLVK VGTGEVKPQE VKEMLQAKDR SRAPFLAPPD 250 GLYLERVFYD DYPY

References

[1]

"Complete sequence of chromosome of Thermovibrio ammonificans HB-1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Vetriani C., Woyke T. Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15698 / JCM 12110 / HB-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002444 Genomic DNA. Translation: ADU96616.1.
RefSeq	YP_004151257.1. NC_014926.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADU96616; ADU96616; Theam_0646.
GeneID	10125683.
KEGG	tam:Theam_0646.
PATRIC	45383617. VBITheAmm13058_0742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000248673.
KO	K06173.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E8T632_THEA1

Accession

Primary (citable) accession number: E8T632

Entry history

Integrated into UniProtKB/TrEMBL:	April 5, 2011
Last sequence update:	April 5, 2011
Last modified:	May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)