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E8S698 (E8S698_MICSL) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:ML5_6251 EMBL ADU11694.1
OrganismMicromonospora sp. (strain L5) [Complete proteome] [HAMAP] EMBL ADU11694.1
Taxonomic identifier648999 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeMicromonospora

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain117 – 324208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding151 – 20656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2781Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2911Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2911Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2931Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E8S698 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: F8FF0E684E3DB49A

FASTA33134,465
        10         20         30         40         50         60 
MSDSPAAPPP VTGSAADELH VLVLAGGLSY ERDVSLRSGR RVLDALRAVG VDAELRDADV 

        70         80         90        100        110        120 
ALLPALTADP PDAVVIALHG ATGEDGSLRG VLDLWDVPYV GCDARSSRLA WDKPSAKAVL 

       130        140        150        160        170        180 
REAGIPTPDW VALPHDRFSE LGAVAVLDRI VDRLGLPLMV KPAQGGSGLG AAVVREAASL 

       190        200        210        220        230        240 
PAAMVGCFAY DQTALVERYV PGMDVAVSVL DLGKGPQALP PVEIVPRNGV YDYAARYTAG 

       250        260        270        280        290        300 
RTTWHAPARL DAATTARVTE VALAAHTALG LRDVSRVDLI VDAGGDPHVL EVNVSPGMTE 

       310        320        330 
TSLLPLAIQA AGLDFGRVLG TLVTRAAARR R 

« Hide

References

[1]"Complete sequence of Micromonospora sp. L5."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Saunders E., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G., De Hoff P.L., Hirsch A.M., Woyke T.
Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002399 Genomic DNA. Translation: ADU11694.1.
RefSeqYP_004085845.1. NC_014815.1.

3D structure databases

ProteinModelPortalE8S698.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADU11694; ADU11694; ML5_6251.
GeneID10061790.
KEGGmil:ML5_6251.
PATRIC45280530. VBIMicSp154089_6274.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMATPDWVAL.

Enzyme and pathway databases

BioCycMSP648999:GHVN-6317-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8S698_MICSL
AccessionPrimary (citable) accession number: E8S698
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: February 19, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)