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E8RA45 (E8RA45_DESM0) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
Short name=PEP carboxykinase HAMAP-Rule MF_00452
Short name=PEPCK HAMAP-Rule MF_00452
EC=4.1.1.32 HAMAP-Rule MF_00452
Alternative name(s):
Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00452
Gene names
Name:pckG HAMAP-Rule MF_00452
Ordered Locus Names:Desmu_0004
OrganismDesulfurococcus mucosus (strain ATCC 35584 / DSM 2162 / JCM 9187 / O7/1) [Complete proteome] [HAMAP] EMBL ADV64323.1
Taxonomic identifier765177 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Desulfurococcus ›

Protein attributes

Sequence length629 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. HAMAP-Rule MF_00452

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region390 – 3923Substrate binding By similarity HAMAP-Rule MF_00452

Sites

Active site2781 By similarity HAMAP-Rule MF_00452
Metal binding2351Manganese By similarity HAMAP-Rule MF_00452
Metal binding2541Manganese By similarity HAMAP-Rule MF_00452
Metal binding2941Manganese By similarity HAMAP-Rule MF_00452
Binding site921Substrate By similarity HAMAP-Rule MF_00452
Binding site2281Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00452
Binding site2351Substrate By similarity HAMAP-Rule MF_00452
Binding site3921GTP By similarity HAMAP-Rule MF_00452
Binding site4231GTP By similarity HAMAP-Rule MF_00452

Sequences

Sequence LengthMass (Da)Tools
E8RA45 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: A6F7264A09719A7B

FASTA62971,760
        10         20         30         40         50         60 
MDASVTGLLT RLSRYADERA VERLSRISDR RLLEWITEVV ELLEPASVFV NTGSREDLEY 

        70         80         90        100        110        120 
IRMRAVEKRE EIPSRYNPRH TVHFDGIYDL ARDRENTRIL TPGGSPIPMV NTGNRDEGLR 

       130        140        150        160        170        180 
ELREIYRGIM RGKEMYIGFY CFGPRGSPFT LYGVQVTDSA YVMHSENILY RVCYDVFVEK 

       190        200        210        220        230        240 
GGSMRYMRFL HSAGELNEYG WSRNISKRRI YIDTVDNITY SVNTQYAGNT VGLKKLSLRL 

       250        260        270        280        290        300 
CVNQGYRENW LCEHMFIVGV KGTGDTISYF TGAFPAGCGK TSTALIADTV VGDDLAMIRN 

       310        320        330        340        350        360 
VNGEARAVNP EIGMFGIIDG VNPVDDPEIY GILSSRETEV IFSNVLLTED GEVWWNGKPS 

       370        380        390        400        410        420 
EPRRGVNYAG EWWPGKLDEK GRPIPPSHPN ARFTTSIFYL SKVDPRINDP NGVPVSGMIF 

       430        440        450        460        470        480 
GGRDSDTWVP VEEAFNWVHG IVTKGASLES ERTTAVLGKA GEREFNPFAI LDFLSVSPGE 

       490        500        510        520        530        540 
FIELHFRFGD GLEKQPRIYG VNYFLRDEAG RYLTEKVDKR VWLKWMALRV NNEVDALETP 

       550        560        570        580        590        600 
TGLIPVYEDL AVLFKRELGK EFSIELYEKL FTVRVGKHLE KAERILRIYE NIPGTPRLFF 

       610        620 
DTLIEQKRRL KHALDKYGMF ISPLSLDKR

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References

[1]"The complete genome of Desulfurococcus mucosus DSM 2162."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Ovchinnikova G., Chertkov O., Held B., Brettin T., Detter J.C., Tapia R., Han C., Land M. expand/collapse author list , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Wirth R., Bilek Y., Hader T., Klenk H.-P., Eisen J.A.
Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35584 / DSM 2162 / JCM 9187 / O7/1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002363 Genomic DNA. Translation: ADV64323.1.
RefSeqYP_004175805.1. NC_014961.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV64323; ADV64323; Desmu_0004.
GeneID10152681.
KEGGdmu:Desmu_0004.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.

Enzyme and pathway databases

BioCycDMUC765177:GHQE-4-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameE8RA45_DESM0
AccessionPrimary (citable) accession number: E8RA45
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info