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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Desulfurococcus mucosus (strain ATCC 35584 / DSM 2162 / JCM 9187 / O7/1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. no protein annotated in this organism
  4. Enolase (eno)
  5. Pyruvate kinase (Desmu_1237)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NAD; via amide nitrogenUniRule annotation1
Active sitei140NucleophileUniRule annotation1
Binding sitei168NADUniRule annotation1
Binding sitei302NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

NADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDHUniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gapUniRule annotation
Ordered Locus Names:Desmu_0845Imported
OrganismiDesulfurococcus mucosus (strain ATCC 35584 / DSM 2162 / JCM 9187 / O7/1)Imported
Taxonomic identifieri765177 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus
Proteomesi
  • UP000001068 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi765177.Desmu_0845.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 140Gp_dh_NInterPro annotationAdd BLAST138

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glyceraldehyde 3-phosphate bindingUniRule annotation3
Regioni194 – 195Glyceraldehyde 3-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiVTVPSHH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E8R9H3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKIAVNGY GTIGKRIVDA VLSNKDFRLV GIAKYRVDYS AYLAARKGIP
60 70 80 90 100
IYVPRERASE FREAGLEPHG YVDYLFEEAD LIYDASPGGK GAGNKKIYES
110 120 130 140 150
YGKPGVFQGG EEPGVAELSY STLCNFEEAL NKKYVRVVSC NTTGMLRLLC
160 170 180 190 200
TLHREYGVKR ALSVIIRRAA DPKEDQRGPV NSILLDPPSI PSHHGLDART
210 220 230 240 250
VAPWLDIVTA AAVVPTTLMH MQFIEVELST QVNRGDVVEL LDGINRFLLV
260 270 280 290 300
ESASTGIDST SKVMELARDS GRPRGDIYEN IVFIDSLSIN ASRLYLFQGI
310 320 330 340 350
HQESIVIPEN IDVAYAMLGI ESDPLKVMER VDSTLGIGGL RRFISPHGYI

QIGGAP
Length:356
Mass (Da):39,090
Last modified:April 5, 2011 - v1
Checksum:i6384484C0E4929EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002363 Genomic DNA. Translation: ADV65149.1.
RefSeqiWP_013562371.1. NC_014961.1.

Genome annotation databases

EnsemblBacteriaiADV65149; ADV65149; Desmu_0845.
GeneIDi10153542.
KEGGidmu:Desmu_0845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002363 Genomic DNA. Translation: ADV65149.1.
RefSeqiWP_013562371.1. NC_014961.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi765177.Desmu_0845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADV65149; ADV65149; Desmu_0845.
GeneIDi10153542.
KEGGidmu:Desmu_0845.

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiVTVPSHH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE8R9H3_DESM0
AccessioniPrimary (citable) accession number: E8R9H3
Entry historyi
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: November 30, 2016
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.