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E8R9H3 (E8R9H3_DESM0) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase HAMAP-Rule MF_00559

Short name=GAPDH HAMAP-Rule MF_00559
EC=1.2.1.59 HAMAP-Rule MF_00559
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase HAMAP-Rule MF_00559
Gene names
Name:gap HAMAP-Rule MF_00559
Ordered Locus Names:Desmu_0845 EMBL ADV65149.1
OrganismDesulfurococcus mucosus (strain ATCC 35584 / DSM 2162 / JCM 9187 / O7/1) [Complete proteome] [HAMAP] EMBL ADV65149.1
Taxonomic identifier765177 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. RuleBase RU003388 HAMAP-Rule MF_00559 SAAS SAAS006436

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. RuleBase RU003388 HAMAP-Rule MF_00559 SAAS SAAS006436

Subunit structure

Homotetramer By similarity. RuleBase RU003388 HAMAP-Rule MF_00559 SAAS SAAS006436

Subcellular location

Cytoplasm By similarity RuleBase RU003388 HAMAP-Rule MF_00559 SAAS SAAS006436.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. HAMAP-Rule MF_00559

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding12 – 132NAD By similarity HAMAP-Rule MF_00559
Region139 – 1413Glyceraldehyde 3-phosphate binding By similarity HAMAP-Rule MF_00559
Region194 – 1952Glyceraldehyde 3-phosphate binding By similarity HAMAP-Rule MF_00559

Sites

Active site1401Nucleophile By similarity HAMAP-Rule MF_00559 PIRSR PIRSR000149-1
Binding site1101NAD; via amide nitrogen By similarity HAMAP-Rule MF_00559
Binding site1681NAD By similarity HAMAP-Rule MF_00559
Binding site3021NAD; via carbonyl oxygen By similarity HAMAP-Rule MF_00559

Sequences

Sequence LengthMass (Da)Tools
E8R9H3 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 6384484C0E4929EA

FASTA35639,090
        10         20         30         40         50         60 
MPVKIAVNGY GTIGKRIVDA VLSNKDFRLV GIAKYRVDYS AYLAARKGIP IYVPRERASE 

        70         80         90        100        110        120 
FREAGLEPHG YVDYLFEEAD LIYDASPGGK GAGNKKIYES YGKPGVFQGG EEPGVAELSY 

       130        140        150        160        170        180 
STLCNFEEAL NKKYVRVVSC NTTGMLRLLC TLHREYGVKR ALSVIIRRAA DPKEDQRGPV 

       190        200        210        220        230        240 
NSILLDPPSI PSHHGLDART VAPWLDIVTA AAVVPTTLMH MQFIEVELST QVNRGDVVEL 

       250        260        270        280        290        300 
LDGINRFLLV ESASTGIDST SKVMELARDS GRPRGDIYEN IVFIDSLSIN ASRLYLFQGI 

       310        320        330        340        350 
HQESIVIPEN IDVAYAMLGI ESDPLKVMER VDSTLGIGGL RRFISPHGYI QIGGAP 

« Hide

References

[1]"The complete genome of Desulfurococcus mucosus DSM 2162."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Ovchinnikova G., Chertkov O., Held B., Brettin T., Detter J.C., Tapia R., Han C., Land M. expand/collapse author list , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Wirth R., Bilek Y., Hader T., Klenk H.-P., Eisen J.A.
Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35584 / DSM 2162 / JCM 9187 / O7/1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002363 Genomic DNA. Translation: ADV65149.1.
RefSeqYP_004176631.1. NC_014961.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV65149; ADV65149; Desmu_0845.
GeneID10153542.
KEGGdmu:Desmu_0845.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000223361.
KOK00150.
OMAVTVPSHH.

Enzyme and pathway databases

BioCycDMUC765177:GHQE-865-MONOMER.
UniPathwayUPA00109; UER00184.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00559. G3P_dehdrog_arch.
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8R9H3_DESM0
AccessionPrimary (citable) accession number: E8R9H3
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: June 11, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)