Skip Header

Contribute Send feedback
Read comments (?) or add your own

E8PN40 (E8PN40_THESS) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
EC=4.2.1.33 HAMAP-Rule MF_01026
Alternative name(s):
Alpha-IPM isomerase HAMAP-Rule MF_01026
Isopropylmalate isomerase HAMAP-Rule MF_01026
Gene names
Name:leuC HAMAP-Rule MF_01026 EMBL ADW21392.1
Ordered Locus Names:TSC_c07650 EMBL ADW21392.1
OrganismThermus scotoductus (strain ATCC 700910 / SA-01) [Complete proteome] [HAMAP] EMBL ADW21392.1
Taxonomic identifier743525 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026 SAAS SAAS004430

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026 SAAS SAAS004430

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026 SAAS SAAS015931

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026 SAAS SAAS004430

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026 SAAS SAAS004430

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily. HAMAP-Rule MF_01026

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding3461Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01026
Metal binding4061Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01026
Metal binding4091Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01026

Sequences

Sequence LengthMass (Da)Tools
E8PN40 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 3610A422984EBCAD

FASTA47051,430
        10         20         30         40         50         60 
MGKTLYEKVW EAHEVRKLRS GQSQLFIDLH LLHEVTSPQA FGMLRDLGLK VRYPHRTFAT 

        70         80         90        100        110        120 
VDHIVPTHDR TEPFQDPLAQ SMLEALRQNT REHGITFFDL GSGNQGIVHV IGPQLGLTQP 

       130        140        150        160        170        180 
GMTIACGDSH TSTHGAFGAV AFGIGTSQVR DVLATQTLAA QKLKVRRINI EGKLSPGAYA 

       190        200        210        220        230        240 
KDVILHIIRH LGVKGGLGYA YEYGGSTVEA MDMESRMTLC NMSIEGGARI GYVNPDETTF 

       250        260        270        280        290        300 
AYLEGRPYSP KGAEWEEAKR RWKSFASDPD AHYDDVVTFQ AEEIPPTVTW GINPGQAVPI 

       310        320        330        340        350        360 
DGRIPLLEEL SPEERPVAEE ALAYMGLKPG QPIKGVPIQV AFIGSCTNAR LSDLREVARY 

       370        380        390        400        410        420 
LKGHKVKKGV RALVVPGSEW VARKAEEEGI AEIFREAGFE WRNPGCSMCL AMNPDRLQGD 

       430        440        450        460        470 
ELAASSSNRN YKGRMGSPKG RTVLMSPLMV AAAAVAGEIA DAREVFGIGR

« Hide

References

[1]"The genome sequence of Thermus scotoductus SA-01."
Gounder K., Liesegang H., Brzuszkiewicz E., Wollherr A., Daniel R., Gottschalk G., van Heerden E., Litthauer D.
Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700910 / SA-01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001962 Genomic DNA. Translation: ADW21392.1.
RefSeqYP_004201940.1. NC_014974.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADW21392; ADW21392; TSC_c07650.
GeneID10176885.
KEGGtsc:TSC_c07650.
PATRIC47044371. VBITheSco147128_0731.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000226972.
KOK01703.

Enzyme and pathway databases

UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. Aconitase_N. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8PN40_THESS
AccessionPrimary (citable) accession number: E8PN40
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 1, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info