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E8NDE3 (E8NDE3_MICTS) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103
Short name=Fapy-DNA glycosylase HAMAP-Rule MF_00103
EC=3.2.2.23 HAMAP-Rule MF_00103
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM HAMAP-Rule MF_00103
Gene names
Name:mutM HAMAP-Rule MF_00103
Synonyms:fpg HAMAP-Rule MF_00103
Ordered Locus Names:MTES_3245 EMBL BAJ76209.1
OrganismMicrobacterium testaceum (strain StLB037) [Complete proteome] [HAMAP] EMBL BAJ76209.1
Taxonomic identifier979556 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Microbacterium ›

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103 SAAS SAAS015887

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103 SAAS SAAS015887

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103 SAAS SAAS015887

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103 SAAS SAAS015887

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103 SAAS SAAS015887

Sequence similarities

Belongs to the FPG family. HAMAP-Rule MF_00103

Contains 1 FPG-type zinc finger. HAMAP-Rule MF_00103 SAAS SAAS015887

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Zinc finger260 – 29435FPG-type By similarity HAMAP-Rule MF_00103

Sites

Active site21Schiff-base intermediate with DNA By similarity HAMAP-Rule MF_00103
Active site31Proton donor By similarity HAMAP-Rule MF_00103
Active site611Proton donor; for beta-elimination activity By similarity HAMAP-Rule MF_00103
Active site2841Proton donor; for delta-elimination activity By similarity HAMAP-Rule MF_00103
Binding site951DNA By similarity HAMAP-Rule MF_00103
Binding site1181DNA By similarity HAMAP-Rule MF_00103

Sequences

Sequence LengthMass (Da)Tools
E8NDE3 [UniParc].

Last modified April 5, 2011. Version 1.
Checksum: 8D183A26F5608AE6

FASTA29632,155
        10         20         30         40         50         60 
MPELPEVEVV RAGLEPAVTG ALITSVDVRD ERALTRHTGG AAHFEAELTG RRIDAAVRRG 

        70         80         90        100        110        120 
KFLWMPVSAD EAIVTHLGMS GQMLLRVPGA PEERHERIRI ELEHPVHGPL SVVFADQRTF 

       130        140        150        160        170        180 
GSLAVDRLVD TPDGAAAGRG SSLPRVPTQV AHIARDPLDP AFDVSRFRSR VGRTASGIKR 

       190        200        210        220        230        240 
VLLDQTVASG IGNIYADESL WAARIHPEAV ASSLPTRAVN RLLGEVREVL DKALAEGGTS 

       250        260        270        280        290 
FDAQYVNVNG QAGYFAHSLN AYGRTGQPCP RCGRPIVRVS FMNRSSHFCP HCQRLR

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of Microbacterium testaceum StLB037, an N-acylhomoserine lactone-degrading bacterium isolated from potato leaves."
Morohoshi T., Wang W.-Z., Someya N., Ikeda T.
J. Bacteriol. 193:2072-2073(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: StLB037 EMBL BAJ76209.1.
[2]"Genome sequence of Microbacterium testaceum StLB037."
Morohoshi T., Wang W.Z., Someya N., Ikeda T.
Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: StLB037.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP012052 Genomic DNA. Translation: BAJ76209.1.
RefSeqYP_004226089.1. NC_015125.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAJ76209; BAJ76209; MTES_3245.
GeneID10219997.
KEGGmts:MTES_3245.
PATRIC46973620. VBIMicTes185510_3226.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000020884.
KOK10563.

Enzyme and pathway databases

BioCycMTES979556:GJFH-4876-MONOMER.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE8NDE3_MICTS
AccessionPrimary (citable) accession number: E8NDE3
Entry history
Integrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 29, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info