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Protein

L-lactate dehydrogenase 2

Gene

ldh2

Organism
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871NADBy similarity
Binding sitei94 – 941SubstrateBy similarity
Binding sitei126 – 1261NAD or substrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Active sitei181 – 1811Proton acceptor
Binding sitei237 – 2371SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 4529NAD2 PublicationsAdd
BLAST
Nucleotide bindingi159 – 17618NAD2 PublicationsAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBLON565042:GIWN-1341-MONOMER.
MetaCyc:MONOMER-13065.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 2 (EC:1.1.1.27)
Short name:
L-LDH 2
Gene namesi
Name:ldh2
Synonyms:ldh
Ordered Locus Names:BLLJ_1290
OrganismiBifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Taxonomic identifieri565042 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000007255 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 320319L-lactate dehydrogenase 2PRO_0000409353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1
320
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 145Combined sources
Helixi18 – 2912Combined sources
Beta strandi34 – 396Combined sources
Helixi43 – 5513Combined sources
Helixi56 – 594Combined sources
Beta strandi64 – 696Combined sources
Helixi71 – 744Combined sources
Beta strandi78 – 825Combined sources
Helixi94 – 11522Combined sources
Beta strandi119 – 1235Combined sources
Helixi128 – 13912Combined sources
Beta strandi145 – 1484Combined sources
Helixi152 – 16615Combined sources
Helixi170 – 1723Combined sources
Beta strandi177 – 1826Combined sources
Helixi189 – 1913Combined sources
Helixi199 – 2013Combined sources
Helixi213 – 23119Combined sources
Helixi239 – 25315Combined sources
Beta strandi258 – 2658Combined sources
Beta strandi267 – 2693Combined sources
Beta strandi274 – 28411Combined sources
Beta strandi287 – 2893Combined sources
Helixi298 – 31619Combined sources
Turni317 – 3193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LLDX-ray2.00A/B2-320[»]
1LTHX-ray2.50R/T2-320[»]
ProteinModelPortaliE8ME30.
SMRiE8ME30. Positions 8-320.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiE8ME30.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiAGDYEDC.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E8ME30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETTVKPTK LAVIGAGAVG STLAFAAAQR GIAREIVLED IAKERVEAEV
60 70 80 90 100
LDMQHGSSFY PTVSIDGSDD PEICRDADMV VITAGPRQKP GQSRLELVGA
110 120 130 140 150
TVNILKAIMP NLVKVAPNAI YMLITNPVDI ATHVAQKLTG LPENQIFGSG
160 170 180 190 200
TNLDSARLRF LIAQQTGVNV KNVHAYIAGE HGDSEVPLWE SATIGGVPMC
210 220 230 240 250
DWTPLPGHDP LDADKREEIH QEVKNAAYKI INGKGATNYA IGMSGVDIIE
260 270 280 290 300
AVLHDTNRIL PVSSMLKDFH GISDICMSVP TLLNRQGVNN TINTPVSDKE
310 320
LAALKRSAET LKETAAQFGF
Length:320
Mass (Da):34,240
Last modified:April 5, 2011 - v1
Checksum:iD3E092AA9C353C4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010888 Genomic DNA. Translation: BAJ66957.1.
AF261669 Genomic DNA. Translation: AAF70510.1.
RefSeqiWP_007052533.1. NC_015067.1.

Genome annotation databases

EnsemblBacteriaiBAJ66957; BAJ66957; BLLJ_1290.
KEGGiblm:BLLJ_1290.
PATRICi46891019. VBIBifLon48544_1337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010888 Genomic DNA. Translation: BAJ66957.1.
AF261669 Genomic DNA. Translation: AAF70510.1.
RefSeqiWP_007052533.1. NC_015067.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LLDX-ray2.00A/B2-320[»]
1LTHX-ray2.50R/T2-320[»]
ProteinModelPortaliE8ME30.
SMRiE8ME30. Positions 8-320.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAJ66957; BAJ66957; BLLJ_1290.
KEGGiblm:BLLJ_1290.
PATRICi46891019. VBIBifLon48544_1337.

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiAGDYEDC.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciBLON565042:GIWN-1341-MONOMER.
MetaCyc:MONOMER-13065.

Miscellaneous databases

EvolutionaryTraceiE8ME30.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b.
  2. Roy D., Sirois S.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-233.
    Strain: ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b.
  3. "Molecular basis of allosteric activation of bacterial L-lactate dehydrogenase."
    Iwata S., Ohta T.
    J. Mol. Biol. 230:21-27(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH NAD.
    Strain: AM 101-2.
  4. "T and R states in the crystals of bacterial L-lactate dehydrogenase reveal the mechanism for allosteric control."
    Iwata S., Kamata K., Yoshida S., Ohta T.
    Nat. Struct. Biol. 1:176-185(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NAD.
    Strain: AM 101-2.

Entry informationi

Entry nameiLDH2_BIFL2
AccessioniPrimary (citable) accession number: E8ME30
Secondary accession number(s): P19869, Q9L505, Q9L506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: April 5, 2011
Last modified: December 9, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.