Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase 2

Gene

ldh2

Organism
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87NADBy similarity1
Binding sitei94SubstrateBy similarity1
Binding sitei126NAD or substrateBy similarity1
Binding sitei157SubstrateBy similarity1
Active sitei181Proton acceptor1
Binding sitei237SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 45NAD2 PublicationsAdd BLAST29
Nucleotide bindingi159 – 176NAD2 PublicationsAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13065.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 2 (EC:1.1.1.27)
Short name:
L-LDH 2
Gene namesi
Name:ldh2
Synonyms:ldh
Ordered Locus Names:BLLJ_1290
OrganismiBifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Taxonomic identifieri565042 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000007255 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004093532 – 320L-lactate dehydrogenase 2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei228PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 14Combined sources5
Helixi18 – 29Combined sources12
Beta strandi34 – 39Combined sources6
Helixi43 – 55Combined sources13
Helixi56 – 59Combined sources4
Beta strandi64 – 69Combined sources6
Helixi71 – 74Combined sources4
Beta strandi78 – 82Combined sources5
Helixi94 – 115Combined sources22
Beta strandi119 – 123Combined sources5
Helixi128 – 139Combined sources12
Beta strandi145 – 148Combined sources4
Helixi152 – 166Combined sources15
Helixi170 – 172Combined sources3
Beta strandi177 – 182Combined sources6
Helixi189 – 191Combined sources3
Helixi199 – 201Combined sources3
Helixi213 – 231Combined sources19
Helixi239 – 253Combined sources15
Beta strandi258 – 265Combined sources8
Beta strandi267 – 269Combined sources3
Beta strandi274 – 284Combined sources11
Beta strandi287 – 289Combined sources3
Helixi298 – 316Combined sources19
Turni317 – 319Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLDX-ray2.00A/B2-320[»]
1LTHX-ray2.50R/T2-320[»]
ProteinModelPortaliE8ME30.
SMRiE8ME30.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiE8ME30.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiMDLMQTA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E8ME30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETTVKPTK LAVIGAGAVG STLAFAAAQR GIAREIVLED IAKERVEAEV
60 70 80 90 100
LDMQHGSSFY PTVSIDGSDD PEICRDADMV VITAGPRQKP GQSRLELVGA
110 120 130 140 150
TVNILKAIMP NLVKVAPNAI YMLITNPVDI ATHVAQKLTG LPENQIFGSG
160 170 180 190 200
TNLDSARLRF LIAQQTGVNV KNVHAYIAGE HGDSEVPLWE SATIGGVPMC
210 220 230 240 250
DWTPLPGHDP LDADKREEIH QEVKNAAYKI INGKGATNYA IGMSGVDIIE
260 270 280 290 300
AVLHDTNRIL PVSSMLKDFH GISDICMSVP TLLNRQGVNN TINTPVSDKE
310 320
LAALKRSAET LKETAAQFGF
Length:320
Mass (Da):34,240
Last modified:April 5, 2011 - v1
Checksum:iD3E092AA9C353C4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010888 Genomic DNA. Translation: BAJ66957.1.
AF261669 Genomic DNA. Translation: AAF70510.1.
RefSeqiWP_007052533.1. NC_015067.1.

Genome annotation databases

EnsemblBacteriaiBAJ66957; BAJ66957; BLLJ_1290.
KEGGiblm:BLLJ_1290.
PATRICi46891019. VBIBifLon48544_1337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010888 Genomic DNA. Translation: BAJ66957.1.
AF261669 Genomic DNA. Translation: AAF70510.1.
RefSeqiWP_007052533.1. NC_015067.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLDX-ray2.00A/B2-320[»]
1LTHX-ray2.50R/T2-320[»]
ProteinModelPortaliE8ME30.
SMRiE8ME30.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAJ66957; BAJ66957; BLLJ_1290.
KEGGiblm:BLLJ_1290.
PATRICi46891019. VBIBifLon48544_1337.

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiMDLMQTA.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciMetaCyc:MONOMER-13065.

Miscellaneous databases

EvolutionaryTraceiE8ME30.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH2_BIFL2
AccessioniPrimary (citable) accession number: E8ME30
Secondary accession number(s): P19869, Q9L505, Q9L506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: April 5, 2011
Last modified: November 2, 2016
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.