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Protein

L-lactate dehydrogenase 2

Gene

ldh2

Organism
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).1 Publication1 Publication

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40NADCombined sources2 Publications1
Binding sitei45NADCombined sources2 Publications1
Binding sitei88SubstrateUniRule annotation1
Binding sitei94SubstrateUniRule annotation1
Binding sitei149NADUniRule annotation1
Binding sitei159Allosteric activatorCombined sources1 Publication1
Active sitei181Proton acceptorCombined sources1 Publication1
Binding sitei237SubstrateCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 19NADCombined sources2 Publications2
Nucleotide bindingi124 – 126NADCombined sources2 Publications3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13065
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 21 Publication (EC:1.1.1.27UniRule annotation)
Short name:
L-LDH 21 Publication
Alternative name(s):
FBP-dependent LDH1 Publication
Gene namesi
Name:ldh2UniRule annotation
Synonyms:ldhUniRule annotation
Ordered Locus Names:BLLJ_1290
OrganismiBifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Taxonomic identifieri565042 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000007255 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004093532 – 320L-lactate dehydrogenase 2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei228PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.2 Publications

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 14Combined sources5
Helixi18 – 29Combined sources12
Beta strandi34 – 39Combined sources6
Helixi43 – 55Combined sources13
Helixi56 – 59Combined sources4
Beta strandi64 – 69Combined sources6
Helixi71 – 74Combined sources4
Beta strandi78 – 82Combined sources5
Helixi94 – 115Combined sources22
Beta strandi119 – 123Combined sources5
Helixi128 – 139Combined sources12
Beta strandi145 – 148Combined sources4
Helixi152 – 166Combined sources15
Helixi170 – 172Combined sources3
Beta strandi177 – 182Combined sources6
Helixi189 – 191Combined sources3
Helixi199 – 201Combined sources3
Helixi213 – 231Combined sources19
Helixi239 – 253Combined sources15
Beta strandi258 – 265Combined sources8
Beta strandi267 – 269Combined sources3
Beta strandi274 – 284Combined sources11
Beta strandi287 – 289Combined sources3
Helixi298 – 316Combined sources19
Turni317 – 319Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLDX-ray2.00A/B2-320[»]
1LTHX-ray2.50R/T2-320[»]
ProteinModelPortaliE8ME30
SMRiE8ME30
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiE8ME30

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 129Substrate bindingCombined sources1 Publication4
Regioni154 – 157Substrate bindingCombined sources1 Publication4
Regioni171 – 176Allosteric activator bindingCombined sources1 Publication6

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E8ME30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETTVKPTK LAVIGAGAVG STLAFAAAQR GIAREIVLED IAKERVEAEV
60 70 80 90 100
LDMQHGSSFY PTVSIDGSDD PEICRDADMV VITAGPRQKP GQSRLELVGA
110 120 130 140 150
TVNILKAIMP NLVKVAPNAI YMLITNPVDI ATHVAQKLTG LPENQIFGSG
160 170 180 190 200
TNLDSARLRF LIAQQTGVNV KNVHAYIAGE HGDSEVPLWE SATIGGVPMC
210 220 230 240 250
DWTPLPGHDP LDADKREEIH QEVKNAAYKI INGKGATNYA IGMSGVDIIE
260 270 280 290 300
AVLHDTNRIL PVSSMLKDFH GISDICMSVP TLLNRQGVNN TINTPVSDKE
310 320
LAALKRSAET LKETAAQFGF
Length:320
Mass (Da):34,240
Last modified:April 5, 2011 - v1
Checksum:iD3E092AA9C353C4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010888 Genomic DNA Translation: BAJ66957.1
AF261669 Genomic DNA Translation: AAF70510.1
RefSeqiWP_007052533.1, NC_015067.1

Genome annotation databases

EnsemblBacteriaiBAJ66957; BAJ66957; BLLJ_1290
KEGGiblm:BLLJ_1290

Similar proteinsi

Entry informationi

Entry nameiLDH2_BIFL2
AccessioniPrimary (citable) accession number: E8ME30
Secondary accession number(s): P19869, Q9L505, Q9L506
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: April 5, 2011
Last modified: April 25, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome
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Main funding by: National Institutes of Health