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Protein

Pheromone-processing carboxypeptidase KEX1

Gene

KEX1

Organism
Saccharomyces cerevisiae (strain FostersO) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from the precursors of K1, K2 and K28 killer toxins and a-factor (mating pheromone). Involved in the programmed cell death caused by defective N-glycosylation and contributes also to the active cell death program induced by acetic acid stress or during chronological aging. Promotes cell fusion by proteolytically processing substrates that act in parallel to PRM1 as an alternative fusion machine, as cell wall components, or both (By similarity).By similarity

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei198By similarity1
Active sitei405By similarity1
Active sitei470By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Apoptosis

Protein family/group databases

ESTHERiyeast-kex01. Carboxypeptidase_S10.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)
Alternative name(s):
Carboxypeptidase D
Killer expression defective protein 1
Gene namesi
Name:KEX1
ORF Names:FOSTERSO_1568
OrganismiSaccharomyces cerevisiae (strain FostersO) (Baker's yeast)
Taxonomic identifieri764101 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000007237 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 625LumenalSequence analysisAdd BLAST603
Transmembranei626 – 646HelicalSequence analysisAdd BLAST21
Topological domaini647 – 738CytoplasmicSequence analysisAdd BLAST92

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000041195623 – 738Pheromone-processing carboxypeptidase KEX1Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi459N-linked (GlcNAc...)Sequence analysis1
Glycosylationi467N-linked (GlcNAc...)Sequence analysis1
Modified residuei669PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi526 – 531Poly-Glu6
Compositional biasi545 – 561Poly-AspAdd BLAST17
Compositional biasi567 – 586Poly-AspAdd BLAST20
Compositional biasi606 – 616Poly-GluAdd BLAST11
Compositional biasi712 – 720Poly-Lys9

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiEOG092C255X.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E7NHF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYNRWLGTW LAMSALIRIS VSLPSSEEYK VAYELLPGLS EVPDPSNIPQ
60 70 80 90 100
MHAGHIPLRS EDADEQDSSD LEYFFWKFTN NDSNGNVDRP LIIWLNGGPG
110 120 130 140 150
CSSMDGALVE SGPFRVNSDG KLYLNEGSWI SKGDLLFIDQ PTGTGFSVEQ
160 170 180 190 200
NKDEGKIDKN KFDEDLEDVT KHFMDFLENY FKIFPEDLTR KIILSGESYA
210 220 230 240 250
GQYIPFFANA ILNHNKFSKI DGDTYDLKAL LIGNGWIDPN TQSLSYLPFA
260 270 280 290 300
MEKKLIDESN PNFKHLTNAH ENCQNLINSA STDEAAHFSY QECENILNLL
310 320 330 340 350
LSYTRESSQK GTADCLNMYN FNLKDSYPSC GMNWPKDVSF VSKFFSTPGV
360 370 380 390 400
IDSLHLDSDK IDHWKECTNS VGTKLSNPIS KPSIHLLPGL LESGIEIVLF
410 420 430 440 450
NGDKDLICNN KGVLDTIDNL KWGGIKGFSD DAVSFDWIHK SKSTDDSEEF
460 470 480 490 500
SGYVKYDRNL TFVSVYNASH MVPFDKSLVS RGIVDIYSND IMIIDNNGKN
510 520 530 540 550
VMITTDDDSD QDATTESGDK PKENLEEEEQ EAQNEEGKEK EGNKDKDGDD
560 570 580 590 600
DNDNDDDDED DHNSEGDDDD DDDDDDDDDD DDDEDDNNEK TKVNQGLEDS
610 620 630 640 650
RHKSSEYEQE EEEVEEFAEE ISMYKHKAVV VTIVTFLIVV LGVYAYDRRV
660 670 680 690 700
RRKARHTILV DPNNRQHDSP NKTVSWADDL ESGLGAEDDL EQDEQLEGGA
710 720 730
PISSTSNKAG SKLKTKKKKK YTSLPNTEID ESFEMTDF
Length:738
Mass (Da):83,279
Last modified:April 5, 2011 - v1
Checksum:iEE0BDADFD4ACF26B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AEEZ01000037 Genomic DNA. Translation: EGA62437.1.

Genome annotation databases

EnsemblFungiiEGA62437; EGA62437; FOSTERSO_1568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AEEZ01000037 Genomic DNA. Translation: EGA62437.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERiyeast-kex01. Carboxypeptidase_S10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEGA62437; EGA62437; FOSTERSO_1568.

Phylogenomic databases

OrthoDBiEOG092C255X.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKEX1_YEASO
AccessioniPrimary (citable) accession number: E7NHF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: April 5, 2011
Last modified: September 7, 2016
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.