ID   E7EU49_HUMAN            Unreviewed;       497 AA.
AC   E7EU49;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   27-MAR-2024, entry version 87.
DE   RecName: Full=Rho guanine nucleotide exchange factor 3 {ECO:0000256|ARBA:ARBA00023847};
GN   Name=ARHGEF3 {ECO:0000313|Ensembl:ENSP00000418826.1};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000418826.1, ECO:0000313|Proteomes:UP000005640};
RN   [1] {ECO:0000313|Ensembl:ENSP00000418826.1, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16641997; DOI=10.1038/nature04728;
RA   Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA   Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA   Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA   Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA   Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA   Sodergren E., Song X.Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA   Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA   Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA   Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA   Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA   Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA   Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA   Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.V., Okwuonu G.O.,
RA   Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA   Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA   Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA   Wang J., Wang Q., Williams G.A., Wong G.K., Yao Z., Zhang J., Zhang X.,
RA   Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA   Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT   "The DNA sequence, annotation and analysis of human chromosome 3.";
RL   Nature 440:1194-1198(2006).
RN   [2] {ECO:0007829|PubMed:23186163}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=23186163;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [3] {ECO:0000313|Ensembl:ENSP00000418826.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) for RhoA
CC       and RhoB GTPases. {ECO:0000256|ARBA:ARBA00043905}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC097358; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC099327; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC099559; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC099781; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; E7EU49; -.
DR   SMR; E7EU49; -.
DR   EPD; E7EU49; -.
DR   MassIVE; E7EU49; -.
DR   MaxQB; E7EU49; -.
DR   PeptideAtlas; E7EU49; -.
DR   ProteomicsDB; 18355; -.
DR   Antibodypedia; 31503; 207 antibodies from 24 providers.
DR   Ensembl; ENST00000497267.5; ENSP00000418826.1; ENSG00000163947.12.
DR   UCSC; uc011bev.2; human.
DR   HGNC; HGNC:683; ARHGEF3.
DR   VEuPathDB; HostDB:ENSG00000163947; -.
DR   GeneTree; ENSGT00940000158385; -.
DR   HOGENOM; CLU_027428_3_0_1; -.
DR   ChiTaRS; ARHGEF3; human.
DR   Proteomes; UP000005640; Chromosome 3.
DR   Bgee; ENSG00000163947; Expressed in renal glomerulus and 194 other cell types or tissues.
DR   ExpressionAtlas; E7EU49; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd10572; PH_RhoGEF3_XPLN; 1.
DR   CDD; cd00160; RhoGEF; 1.
DR   Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR   Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR   InterPro; IPR035899; DBL_dom_sf.
DR   InterPro; IPR000219; DH-domain.
DR   InterPro; IPR001331; GDS_CDC24_CS.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   InterPro; IPR044129; PH_RhoGEF3_XPLN.
DR   PANTHER; PTHR46006:SF2; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 3; 1.
DR   PANTHER; PTHR46006; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A; 1.
DR   Pfam; PF00621; RhoGEF; 1.
DR   SMART; SM00233; PH; 1.
DR   SMART; SM00325; RhoGEF; 1.
DR   SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR   SUPFAM; SSF50729; PH domain-like; 1.
DR   PROSITE; PS00741; DH_1; 1.
DR   PROSITE; PS50010; DH_2; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification {ECO:0007829|EPD:E7EU49,
KW   ECO:0007829|MaxQB:E7EU49};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640}.
FT   DOMAIN          93..275
FT                   /note="DH"
FT                   /evidence="ECO:0000259|PROSITE:PS50010"
FT   DOMAIN          262..420
FT                   /note="PH"
FT                   /evidence="ECO:0000259|PROSITE:PS50003"
FT   REGION          435..497
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        435..450
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        480..497
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   497 AA;  56691 MW;  F8AE7F41098B267C CRC64;
     MIEEPSNKRV KPLSRVTSLA NLIPPVKATP LKRFSQTLQR SISFRSESRP DILAPRPWSR
     NAAPSSTKRR DSKLWSETFD VCVNQMLTSK EIKRQEAIFE LSQGEEDLIE DLKLAKKAYH
     DPMLKLSIMT EQELNQIFGT LDSLIPLHEE LLSQLRDVRK PDGSTEHVGP ILVGWLPCLS
     SYDSYCSNQV AAKALLDHKK QDHRVQDFLQ RCLESPFSRK LDLWNFLDIP RSRLVKYPLL
     LREILRHTPN DNPDQQHLEE AINIIQGIVA EINTKTGESE CRYYKERLLY LEEGQKDSLI
     DSSRVLCCHG ELKNNRGVKL HVFLFQEVLV ITRAVTHNEQ LCYQLYRQPI PVKDLLLEDL
     QDGEVRLGGS LRGAFSNNER IKNFFRVSFK NGSQSQTHSL QANDTFNKQQ WLNCIRQAKE
     TVLCAAGQAG VLDSEGSFLN PTTGSRELQG ETKLEQMDQS DSESDCSMDT SEVSLDCERM
     EQTDSSCGNS RHGESNV
//