E7DN64 (DAMS_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-amyrin synthase
EC=5.4.99.55

Alternative name(s):
Alpha-amyrin synthase
EC=5.4.99.40
Beta-amyrin synthase
EC=5.4.99.39
Triterpenoid synthase 2
Short name=SlTTS2

Gene names

Name:

TTS2

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Multifunctional oxidosqualene cyclase producing delta-amyrin (48%), alpha-amyrin (18%), beta-amyrin (13%) and 4 other minor triterpenes. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = delta-amyrin. Ref.1 (3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin. Ref.1 (3S)-2,3-epoxy-2,3-dihydrosqualene = alpha-amyrin. Ref.1
Tissue specificity	Expressed in the leaves and in the epidermal cells but not in the inner tissues of the fruit. Ref.1
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 5 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	intramolecular transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	Delta-amyrin synthase		PRO_0000413971
Regions
	Repeat	148 – 189	42	PFTB 1
	Repeat	513 – 558	46	PFTB 2
	Repeat	590 – 630	41	PFTB 3
	Repeat	639 – 680	42	PFTB 4
	Repeat	701 – 742	42	PFTB 5

Sequences

	Sequence		Length	Mass (Da)
	E7DN64 [UniParc]. Last modified March 8, 2011. Version 1. Checksum: 0CD81BBC15F49BEC	FASTA	763	88,036
	10 20 30 40 50 60 MWKLKIAKGQ DDRYLYSTNN YIGRQIWEFD PNAGTIEEQA KIEEARQHYW NNRYKVKPNS 70 80 90 100 110 120 DLLWRMQFLR EKNFKQRIRA VKVEEGEEIS HEIATVALHR AVHFFSALQA TDGHWPAESA 130 140 150 160 170 180 GPLFFLPPLV MCMYITGHLN TVFPAEHRKE ILRYIYCHQN EDGGWGLHIE GHSTMFCTAM 190 200 210 220 230 240 SYICMRILGE GPEGGVNNAC ARARKWILDH GSVIAIPSWG KTWLSILGAF EWIGTNPMPP 250 260 270 280 290 300 EFWILPSFLP VHPAKMWCYC RTVYMPMSYL YGKRFVGPIT PLILKLREEL YDQTYDEINW 310 320 330 340 350 360 KKVRHVCAKE DLYYPHPFVQ DLMWDSLYIC TEPLLTRWPF NKLRNKALEV TMKHIHYEDE 370 380 390 400 410 420 NSRYITMGCV EKVLSMLACW VEDPNGDHFK KHLARIPDFL WVAEDGMKMQ GCGSQSWDAS 430 440 450 460 470 480 LAIQALLASE MNDEISDTLK NGHDFIKQSQ VKDNPSGDFK VMYRHISKGS WAFADQDLGW 490 500 510 520 530 540 QVSDCTAEAL KCCLLFSTMP PEIVGEAMDP VRLYDSVNVI LSLQSKNGGL SAWEPAGAPE 550 560 570 580 590 600 YLELLNPTEF FEDIVIEHEH VECTSSAIQA LVRFKKLYPG HRTTEVDNFI NNGVKYIEDV 610 620 630 640 650 660 QEPDGSWYGN WGVCFIYASW FALGGLAAVG LSYSNCAAVR KSVEFLLRTQ RSDGGWGESY 670 680 690 700 710 720 RSCPDKVYRE LETEHSNLVQ TAWALMGLIH SGQVERDPRP LHRAAKLLIN FQMEDGDFPQ 730 740 750 760 QEITGVFLRN CMMHYALYRN IFPLWGLAEY RRNVLVPLKH NYI

References

[1]

"Two oxidosqualene cyclases responsible for biosynthesis of tomato fruit cuticular triterpenoids." Wang Z., Guhling O., Yao R., Li F., Yeats T., Rose J., Jetter R. Plant Physiol. 155:540-552(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY. Strain: cv. MicroTom.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HQ266580 mRNA. Translation: ADU52575.1.
RefSeq	NP_001234597.1. NM_001247668.1.
UniGene	Les.18888.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100529100.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAMS_SOLLC

Accession

Primary (citable) accession number: E7DN64

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	March 8, 2011
Last modified:	March 6, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program