Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Gene

gcpE

Organism
Helicobacter felis (strain ATCC 49179 / NCTC 12436 / CS1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.UniRule annotation

Catalytic activityi

(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin.UniRule annotationSAAS annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi263 – 2631Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi266 – 2661Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi298 – 2981Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi305 – 3051Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
  2. terpenoid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Biological processi

Isoprene biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciHFEL936155:GHMC-1340-MONOMER.
UniPathwayiUPA00056; UER00096.
UPA00056; UER00096.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthaseUniRule annotationSAAS annotation (EC:1.17.7.1UniRule annotationSAAS annotation)
Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthaseUniRule annotation
Gene namesi
Name:gcpEImported
Synonyms:ispGUniRule annotation
Ordered Locus Names:HFELIS_13340Imported
OrganismiHelicobacter felis (strain ATCC 49179 / NCTC 12436 / CS1)Imported
Taxonomic identifieri936155 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000007934: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the IspG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000261019.
KOiK03526.
OMAiHNGMKIA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG.
InterProiIPR013785. Aldolase_TIM.
IPR029065. Enolase_C-like.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
[Graphical view]
PfamiPF04551. GcpE. 1 hit.
[Graphical view]
PIRSFiPIRSF004640. IspG. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.

Sequencei

Sequence statusi: Complete.

E7A9Z9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHPRFPTQQI SIGHVLIGGD APISTQSMTF SQTCDIEATK AQIDRLKLAG
60 70 80 90 100
ADLVRVAVRH KKDALALKEL KKVASLPLIA DIHFHHHLAL IAAESVDAIR
110 120 130 140 150
INPGNIGAPE KIKAVAQACN ARNLPIRIGV NGGSLEKPFE LKYGATPEGM
160 170 180 190 200
VQSALYNIKF LEDAGFTNIK ISLKASDVER TIAAYQQLRP LVPYPFHLGV
210 220 230 240 250
TEAGSLFHSS IKSAMALGQL LREGIGDTMR VSITGELEEE IRVAKAILRY
260 270 280 290 300
SGRQKEGITF ISCPTCGRIE SNLVAMMSQV EQRLAHIKIP LDVSVMGCVV
310 320 330 340 350
NALGEAKHAD IAIAFGKKSG LIIKKGKVIH KLPEDQLLET FVTEVQTLAL
360
EQQKEDIHAK I
Length:361
Mass (Da):39,262
Last modified:March 8, 2011 - v1
Checksum:i2F1C03877B676EB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ670179 Genomic DNA. Translation: CBY83418.1.
RefSeqiYP_004074008.1. NC_014810.2.

Genome annotation databases

EnsemblBacteriaiCBY83418; CBY83418; HFELIS_13340.
GeneIDi10050600.
KEGGihfe:HFELIS_13340.
PATRICi45240607. VBIHelFel178703_1380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ670179 Genomic DNA. Translation: CBY83418.1.
RefSeqiYP_004074008.1. NC_014810.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBY83418; CBY83418; HFELIS_13340.
GeneIDi10050600.
KEGGihfe:HFELIS_13340.
PATRICi45240607. VBIHelFel178703_1380.

Phylogenomic databases

HOGENOMiHOG000261019.
KOiK03526.
OMAiHNGMKIA.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00096.
UPA00056; UER00096.
BioCyciHFEL936155:GHMC-1340-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG.
InterProiIPR013785. Aldolase_TIM.
IPR029065. Enolase_C-like.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
[Graphical view]
PfamiPF04551. GcpE. 1 hit.
[Graphical view]
PIRSFiPIRSF004640. IspG. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative whole genome analysis of the carcinogenic bacterial pathogen Helicobacter felis."
    Arnold A., Zigova Z., Lawley T., Falkow S., Bentley S., Aslett M., Muller A.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49179 / NCTC 12436 / CS1Imported.

Entry informationi

Entry nameiE7A9Z9_HELFC
AccessioniPrimary (citable) accession number: E7A9Z9
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: February 4, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.