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E6X2H8 (E6X2H8_NITSE) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Nitsa_1940 EMBL ADV47183.1
OrganismNitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1) [Complete proteome] [HAMAP] EMBL ADV47183.1
Taxonomic identifier749222 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesNitratifractor

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain136 – 335200ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding162 – 21554ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2871Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3011Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E6X2H8 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 302336630C8898DF

FASTA34939,639
        10         20         30         40         50         60 
MKIAILFGGS SYEHEISIVS AITLKKSLKR NEPLFIFVDS AREFYLIEPE KMKSKTFSSG 

        70         80         90        100        110        120 
EYKKAKKLSL ERGGFSMAGG LLGGRKPLEF DCLLNLIHGR DGEDGKIAGM MEFFRIPFIG 

       130        140        150        160        170        180 
PRLEASVMSY SKLNTKLLAQ ALGISVLEYE LLRKEGPRKL SRLEYPVIVK PVHLGSSIGV 

       190        200        210        220        230        240 
SIVREASQLD YALDVAFEFD DLVIVEPFVE GIREYNLAGC KTSAWELSLI EEPQKEEFLD 

       250        260        270        280        290        300 
FDKKYMDFSR DTRVQEAVID APLRERLETA FKNIYDPLFL GSLIRCDFFV VEEKVYLNEI 

       310        320        330        340 
NPIPGSMAHY LFDDFEGIIE RLAKNLPKEK SIPVDYRYIH SIQSAKGKA 

« Hide

References

[1]"The complete genome of Nitratifractor salsuginis DSM 16511."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Zeytun A., Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V., Cheng J.-F. expand/collapse author list , Hugenholtz P., Woyke T., Wu D., Tindall B., Schuetze A., Brambilla E., Klenk H.-P., Eisen J.A.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16511 / JCM 12458 / E9I37-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002452 Genomic DNA. Translation: ADV47183.1.
RefSeqYP_004168932.1. NC_014935.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV47183; ADV47183; Nitsa_1940.
GeneID10148740.
KEGGnsa:Nitsa_1940.
PATRIC46985417. VBINitSal150288_1918.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000102494.
KOK01921.

Enzyme and pathway databases

BioCycNSAL749222:GHWN-1981-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6X2H8_NITSE
AccessionPrimary (citable) accession number: E6X2H8
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: June 11, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)