Skip Header

Contribute Send feedback
Read comments (?) or add your own

E6WZ78 (E6WZ78_NITSE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_01228
EC=6.1.1.10 HAMAP-Rule MF_01228
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_01228
Gene names
Name:metG HAMAP-Rule MF_01228
Ordered Locus Names:Nitsa_1339
OrganismNitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1) [Complete proteome] [HAMAP] EMBL ADV46590.1
Taxonomic identifier749222 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Nitratifractor ›

Protein attributes

Sequence length651 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228 SAAS SAAS002547

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). SAAS SAAS002547 HAMAP-Rule MF_01228

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01228

Subunit structure

Homodimer By similarity. SAAS SAAS002547

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. HAMAP-Rule MF_01228

Contains 1 tRNA-binding domain. SAAS SAAS002547 HAMAP-Rule MF_01228

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain550 – 651102tRNA-binding By similarity HAMAP-Rule MF_01228
Motif15 – 2511"HIGH" region By similarity HAMAP-Rule MF_01228
Motif301 – 3055"KMSKS" region By similarity HAMAP-Rule MF_01228

Sites

Metal binding1301Zinc By similarity HAMAP-Rule MF_01228
Metal binding1331Zinc By similarity HAMAP-Rule MF_01228
Metal binding1481Zinc By similarity HAMAP-Rule MF_01228
Metal binding1511Zinc By similarity HAMAP-Rule MF_01228

Sequences

Sequence LengthMass (Da)Tools
E6WZ78 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 94A443C508FF1266

FASTA65173,947
        10         20         30         40         50         60 
MSEHCKKAYI TTPIYYVNDI PHIGHAYTTI IADTLAIYSR MAGLETFFLT GTDEHGQKIE 

        70         80         90        100        110        120 
EAAKKRGKSP KEYADEISGR FKALWDEFDI SYDKFIRTTD EDHKLGVQAA FKKMYEKGDI 

       130        140        150        160        170        180 
YKDVYRGHYC ISCEAFFTET QLVDGEFCPE CGKPTQIVEE ESYFFRLSKY QDKLLEWYRE 

       190        200        210        220        230        240 
NEKCILPKTR RNEVMRFVEG GLDDLSITRT SFDWGVKLPP EINDPKHVMY VWLDALMNYL 

       250        260        270        280        290        300 
TALGYGTDEK LLDFWPARVH LIGKDILRFH AVYWPAFLMS LDMPLPKHIA AHGWWTRNGE 

       310        320        330        340        350        360 
KMSKSKGNVI NPKEVADAYG LENFRYFMLR EVPFGGDGDF SQKALIDRIN SDLGNDLGNL 

       370        380        390        400        410        420 
LNRLIGMSGK YSEGSVSSAK VAELYGDELA EVHDSLDKLE PLLFEMQLHR YLEELWKPLS 

       430        440        450        460        470        480 
IANKSIDKYQ PWVLMKEGKT EEALALLGLI ANILAKVSVM LHPVMPKTTD TIARALGFTI 

       490        500        510        520        530        540 
NQTAYEDLIK RKDLLSDFTI EKVPPLFPRI EEQLLKEATP AEEKAAPKKE ASKKEKAKSE 

       550        560        570        580        590        600 
EPQGLITIDQ FFQTSLKVGT VLEAEEVPKS KKLLLLQVDL GEEQPRQIVA GIKEWYAPAD 

       610        620        630        640        650 
LVGTQVCVVA NLKPAKLMGL RSEGMLLAAK DEEGLALIRP EKPKKPGTPI G

« Hide

References

[1]"The complete genome of Nitratifractor salsuginis DSM 16511."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Zeytun A., Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V., Cheng J.-F. expand/collapse author list , Hugenholtz P., Woyke T., Wu D., Tindall B., Schuetze A., Brambilla E., Klenk H.-P., Eisen J.A.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16511 / JCM 12458 / E9I37-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002452 Genomic DNA. Translation: ADV46590.1.
RefSeqYP_004168339.1. NC_014935.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV46590; ADV46590; Nitsa_1339.
GeneID10148129.
KEGGnsa:Nitsa_1339.
PATRIC46984235. VBINitSal150288_1335.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000200401.
KOK01874.

Enzyme and pathway databases

BioCycNSAL749222:GHWN-1370-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6WZ78_NITSE
AccessionPrimary (citable) accession number: E6WZ78
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info