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E6WQ51 (E6WQ51_PSEUU) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase HAMAP-Rule MF_01025
EC=2.3.3.13 HAMAP-Rule MF_01025
Alternative name(s):
Alpha-IPM synthase HAMAP-Rule MF_01025
Alpha-isopropylmalate synthase HAMAP-Rule MF_01025
Gene names
Name:leuA HAMAP-Rule MF_01025
Ordered Locus Names:Psesu_0441
OrganismPseudoxanthomonas suwonensis (strain 11-1) [Complete proteome] [HAMAP] EMBL ADV26300.1
Taxonomic identifier743721 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Pseudoxanthomonas ›

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025 SAAS SAAS000891

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025 SAAS SAAS005671

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025 SAAS SAAS000891

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01025 SAAS SAAS000891

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. HAMAP-Rule MF_01025

Sequences

Sequence LengthMass (Da)Tools
E6WQ51 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 8610F2DB5359D16C

FASTA52456,850
        10         20         30         40         50         60 
MNAQQSQAQT QVSPARIRVF DTTLRDGEQS PGCSMTPPQK LVMARALADL GVDVIETGFP 

        70         80         90        100        110        120 
ASSQSDREAM ALIAREIREP TLAVLSRCLQ ADIDISLRSL EAAAKPRLHV FLSTSPLHRE 

       130        140        150        160        170        180 
HKLRMSRGQV LERVVQSIRY AKQHIDDIEF STEDGTRTEE DFLHEIVGVA IAAGATTINI 

       190        200        210        220        230        240 
PDTVGFTTPE EIRGIFQRLI AQVPGADKVI FSAHCHNDLG LAVANTLAAI EGGARQVECT 

       250        260        270        280        290        300 
INGIGERAGN CALEEIVMAL KVRNAFFEAD TGIDTRRLVP TSQLLQRLIG MPVQRNKAVV 

       310        320        330        340        350        360 
GSNAFAHESG IHQHGMLRHR GTYEIMRPQD VGWPDTQMVL GRHSGRAAVE QRLRALGYYL 

       370        380        390        400        410        420 
EEAELDMVFA DFKALCEQQR VVTDADLQHL MQDTGDGEGY RLASMTVSDV GQGASATVEL 

       430        440        450        460        470        480 
SDPDGRRVAE TAKGDGPVDA LFAALSAATG VQLSLESYQV HSVGLGTDAR GEASLSVRHG 

       490        500        510        520 
EDEYEGTGTS RDIIEASALA WLDVANRLLR QRRATGNSEQ LATA

« Hide

References

[1]"Complete sequence of Pseudoxanthomonas suwonensis 11-1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Siebers A.K., Allgaier M., Thelen M.P. expand/collapse author list , Hugenholtz P., Gladden J., Woyke T.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 11-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002446 Genomic DNA. Translation: ADV26300.1.
RefSeqYP_004145531.1. NC_014924.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADV26300; ADV26300; Psesu_0441.
GeneID10121983.
KEGGpsu:Psesu_0441.
PATRIC45334638. VBIPseSuw172708_0458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000046859.
KOK01649.
OMATKEYPAT.

Enzyme and pathway databases

BioCycPSUW743721:GH68-443-MONOMER.
UniPathwayUPA00048; UER00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01025. LeuA_type1.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMsTIGR00973. leuA_bact. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6WQ51_PSEUU
AccessionPrimary (citable) accession number: E6WQ51
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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