ID   E6WLB3_PANSA            Unreviewed;       492 AA.
AC   E6WLB3;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   27-MAR-2024, entry version 62.
DE   SubName: Full=Glucan 1,4-alpha-maltohexaosidase {ECO:0000313|EMBL:ADU72198.1};
DE            EC=3.2.1.98 {ECO:0000313|EMBL:ADU72198.1};
GN   OrderedLocusNames=Pat9b_4889 {ECO:0000313|EMBL:ADU72198.1};
OS   Pantoea sp. (strain At-9b).
OG   Plasmid pPAT9B02 {ECO:0000313|EMBL:ADU72198.1,
OG   ECO:0000313|Proteomes:UP000001624}.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=592316 {ECO:0000313|EMBL:ADU72198.1, ECO:0000313|Proteomes:UP000001624};
RN   [1] {ECO:0000313|EMBL:ADU72198.1, ECO:0000313|Proteomes:UP000001624}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=At-9b {ECO:0000313|EMBL:ADU72198.1,
RC   ECO:0000313|Proteomes:UP000001624};
RC   PLASMID=Plasmid pPAT9B02 {ECO:0000313|Proteomes:UP000001624};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA   Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.;
RT   "Complete sequence plasmid2 of Pantoea sp. At-9b.";
RL   Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; CP002435; ADU72198.1; -; Genomic_DNA.
DR   RefSeq; WP_013512027.1; NC_014839.1.
DR   AlphaFoldDB; E6WLB3; -.
DR   KEGG; pao:Pat9b_4889; -.
DR   HOGENOM; CLU_024572_2_0_6; -.
DR   OrthoDB; 9805159at2; -.
DR   Proteomes; UP000001624; Plasmid pPAT9B02.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0033927; F:glucan 1,4-alpha-maltohexaosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR   Gene3D; 2.40.30.140; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Glycosidase {ECO:0000313|EMBL:ADU72198.1};
KW   Hydrolase {ECO:0000313|EMBL:ADU72198.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Plasmid {ECO:0000313|EMBL:ADU72198.1}.
FT   DOMAIN          4..402
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        235
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        265
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         198
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         206
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         239
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   492 AA;  56247 MW;  2EC1CF03E008224D CRC64;
     MKNPTLLQAF HWYYPEGGQL WPKLAERAAH MSESGINMIW LPPAYKGASG GSSVGYDTYD
     LFDLGEFDQK GSIATKYGDK AQLHTAIEAL HQHQIAVLMD VVVNHKMGAD ERERILVNRV
     DEQDRSQIHE EVVECDAWTR YTFPVRAGKY SKFIWDYKCF SGVDHIENPD EDGVFKIVND
     YTGEGWNDQV DNELGNFDYL MGSNIDFRNR SATEEIKYWA RWMMETTQCD GFRLDAVKHI
     PAWFYKEWID HVQEVSEKQL FIVAEYWSFE LDKLQQYINQ VEGKTLLFDA PLHMKFHEAS
     KQGSSYDMSQ IFSGTLVEAD PFHAVTIVAN HDTQPLQALE APVEAWFKPL AYALILLREN
     GVPCVFYPDL YGASYEDIGS DGEKHKVEMP VIDKLDRLMI ARQRFAHGVQ TLWFDHPNCI
     AFSRSGTEDE TGCVVIMSNG DYGEKTLMLG ENYAGKNWRD FLGNRDETIT TNEQGEAVFT
     CSGGSVSVWV ID
//