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Protein

Glutaminase

Gene

glsA

Organism
Pantoea sp. (strain At-9b)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei160 – 1601SubstrateUniRule annotation
Binding sitei204 – 2041SubstrateUniRule annotation
Binding sitei211 – 2111SubstrateUniRule annotation
Binding sitei235 – 2351SubstrateUniRule annotation
Binding sitei287 – 2871SubstrateUniRule annotation
Binding sitei305 – 3051Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPSP592316:GI0L-4214-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GlutaminaseUniRule annotationSAAS annotation (EC:3.5.1.2UniRule annotationSAAS annotation)
Gene namesi
Name:glsAUniRule annotation
Ordered Locus Names:Pat9b_5237Imported
Encoded oniPlasmid pPAT9B01Imported
OrganismiPantoea sp. (strain At-9b)Imported
Taxonomic identifieri592316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea
ProteomesiUP000001624: Plasmid pPAT9B01

PTM / Processingi

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE6WJY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000216890.
KOiK01425.
OMAiVNSIMAT.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

E6WJY4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAENMLHCNA GFTLIAHSFL LHQLTFNTQI PIIPAQNGYA RSITVSELSN
60 70 80 90 100
ALLEEIIEEV RPLTRQGKVA DYIPALAGIS PDQLGMAIYA ADGSHYQAGD
110 120 130 140 150
ADTRFSIQSI SKVLSLTVAL TLYDEPEIWQ RVGKEPSGQA FNSLVQLELE
160 170 180 190 200
QGKPRNPFIN AGALVMCDLL ESRLTAPRLR MLEIVRRLAQ QNDINYDRVV
210 220 230 240 250
ARSEYEHSAR NAAIAWLMKS FGNFHNDVSK VMETYFHYCA LAMSCNELAR
260 270 280 290 300
TFFYLANHGR SLDDDITLIS PRHARQVNAL MMTSGMYDGA GEFAWRVGMP
310 320 330 340 350
AKSGVGGGIV AVIPGAMSIA VWSPALDSSG NSLAGTVMLE KLTERLGRSI

F
Length:351
Mass (Da):38,587
Last modified:March 8, 2011 - v1
Checksum:i4B4E563080D8B02E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002434 Genomic DNA. Translation: ADU71396.1.
RefSeqiWP_013511227.1. NC_014838.1.
YP_004117952.1. NC_014838.1.

Genome annotation databases

EnsemblBacteriaiADU71396; ADU71396; Pat9b_5237.
GeneIDi10075411.
KEGGipao:Pat9b_5237.
PATRICi45322701. VBIPanSp129740_4108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002434 Genomic DNA. Translation: ADU71396.1.
RefSeqiWP_013511227.1. NC_014838.1.
YP_004117952.1. NC_014838.1.

3D structure databases

ProteinModelPortaliE6WJY4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADU71396; ADU71396; Pat9b_5237.
GeneIDi10075411.
KEGGipao:Pat9b_5237.
PATRICi45322701. VBIPanSp129740_4108.

Phylogenomic databases

HOGENOMiHOG000216890.
KOiK01425.
OMAiVNSIMAT.

Enzyme and pathway databases

BioCyciPSP592316:GI0L-4214-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence plasmid1 of Pantoea sp. At-9b."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiE6WJY4_PANSA
AccessioniPrimary (citable) accession number: E6WJY4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: February 4, 2015
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, PlasmidImported, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.