E6WGD1 (E6WGD1_PANSA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase M HAMAP-Rule MF_01551
EC=2.1.1.186 HAMAP-Rule MF_01551

Alternative name(s):
23S rRNA (cytidine2498-2'-O)-methyltransferase HAMAP-Rule MF_01551
23S rRNA 2'-O-ribose methyltransferase RlmM HAMAP-Rule MF_01551

Gene names

Name:	rlmM HAMAP-Rule MF_01551
Ordered Locus Names:	Pat9b_3113 EMBL ADU70411.1

Organism

Pantoea sp. (strain At-9b) [Complete proteome] [HAMAP] EMBL ADU70411.1

Taxonomic identifier

592316 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pantoea

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA By similarity. HAMAP-Rule MF_01551 SAAS SAAS002877
Catalytic activity	S-adenosyl-L-methionine + cytidine(2498) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(2498) in 23S rRNA. HAMAP-Rule MF_01551 SAAS SAAS002877
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01551 SAAS SAAS002877
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01551 SAAS SAAS002877.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily. HAMAP-Rule MF_01551

Ontologies

Keywords
Biological process	rRNA processing HAMAP-Rule MF_01551 SAAS SAAS002877
Cellular component	Cytoplasm HAMAP-Rule MF_01551 SAAS SAAS002877
Ligand	S-adenosyl-L-methionine HAMAP-Rule MF_01551 SAAS SAAS002877
Molecular function	Methyltransferase HAMAP-Rule MF_01551 SAAS SAAS002877 EMBL ADU70411.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	221 – 224	4	S-adenosyl-L-methionine binding By similarity HAMAP-Rule MF_01551
Sites
	Active site	306	1	Proton acceptor By similarity HAMAP-Rule MF_01551
	Binding site	188	1	S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01551
	Binding site	240	1	S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01551
	Binding site	260	1	S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01551
	Binding site	277	1	S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01551

Sequences

	Sequence		Length	Mass (Da)
	E6WGD1 [UniParc]. Last modified March 8, 2011. Version 1. Checksum: 97FCA18D847857B8	FASTA	366	41,789
	10 20 30 40 50 60 MNKVLLYCRP GFEKECAAEI SAKAAEREIY GFPRVKDDAG YVLFECYQQE DAEKLISQLP 70 80 90 100 110 120 FAELIFARQM VVVGELLRDL PPEDRISPIV GMLTGVVEKG GELRVEVPDT NEAKELTKFC 130 140 150 160 170 180 RKFTVPLRAS LRESKILLNY ESPKRPVVHV FFIAPGTCYV GYSLPQNNSP FYMGIPRLKF 190 200 210 220 230 240 PSDAPSRSTL KLEEAFHVFI PADEWDERLG SGMWAVDLGA CPGGWTYQLV KRSMMVYAVD 250 260 270 280 290 300 NGPMAPSLMD TGQVTHCRED GFRFRPPRNN ISWLVCDMVE KPVRVASLMS EWLVNGWCRE 310 320 330 340 350 360 AIFNLKLPMK KRYEEVTQNL AMIDATLKEQ GINAQIQARQ LYHDREEVTV HVRRIWASVG GRRDER

References

[1]

"Complete sequence chromosome of Pantoea sp. At-9b." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T. Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: At-9b EMBL ADU70411.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002433 Genomic DNA. Translation: ADU70411.1.
RefSeq	YP_004116967.1. NC_014837.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADU70411; ADU70411; Pat9b_3113.
GeneID	10070571.
KEGG	pao:Pat9b_3113.
PATRIC	45320650. VBIPanSp129740_3112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000247137.
KO	K06968.
Enzyme and pathway databases
BioCyc	PSP592316:GI0L-5095-MONOMER.
Family and domain databases
HAMAP	MF_01551. 23SrRNA_methyltr_M.
InterPro	IPR002877. rRNA_MeTrfase_FtsJ_dom. IPR011224. rRNA_MeTrfase_M. [Graphical view]
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF028774. UCP028774. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E6WGD1_PANSA

Accession

Primary (citable) accession number: E6WGD1

Entry history

Integrated into UniProtKB/TrEMBL:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	May 1, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)