E6WG76 (E6WG76_PANSA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897

Gene names

Name:	gyrA HAMAP-Rule MF_01897
Ordered Locus Names:	Pat9b_2700 EMBL ADU70000.1

Organism

Pantoea sp. (strain At-9b) [Complete proteome] [HAMAP] EMBL ADU70000.1

Taxonomic identifier

592316 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pantoea

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_01897
Ligand	ATP-binding SAAS SAAS013760 HAMAP-Rule MF_01897 DNA-binding SAAS SAAS005743 HAMAP-Rule MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005743 HAMAP-Rule MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

	Sequence		Length	Mass (Da)
	E6WG76 [UniParc]. Last modified March 8, 2011. Version 1. Checksum: 5575E23529BE2A67	FASTA	879	97,372
	10 20 30 40 50 60 MSDLAREITP VNIEEELKNS YLDYAMSVIV GRALPDVRDG LKPVHRRVLF AMSELGNDWN 70 80 90 100 110 120 KPYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM 130 140 150 160 170 180 RYTEVRMAKI AHDLLADLEK ETVDFVPNYD GTEQIPEVLP TKIPNLLVNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLREV INGCLAYIDD ENISVEGLME HIPGPDFPTA AIINGRRGIE EAYRTGRGKI 250 260 270 280 290 300 YIRARGEVEA DAKSGRETII IHELPYQVNK ARLIEKIAEL VKEKRVEGIS ALRDESDKDG 310 320 330 340 350 360 MRIVIEIKRD AVGEVVLNNL YSLTQLQVSF GINMVALHQG QPKIMALKEI LEAFVRHRRE 370 380 390 400 410 420 VVTRRTIFEL RKARDRAHIL EGLAISLANI DPIIELIRRA PTPAEAKAGL IAQSWDLGNV 430 440 450 460 470 480 AAMLERAGDD AARPEWLEAE FGIRDGRYYL TEQQAQAILD LRLQKLTGLE HEKLLDEYKE 490 500 510 520 530 540 LLEQIAELLH ILASAERLME VIREELEAMR DQFGDDRRTE ITANSADINI EDLINQEDVV 550 560 570 580 590 600 VTLSHQGYVK YQPLSDYEAQ RRGGKGKSAA RIKEEDFIDR LLVANTHDTI LCFSSRGRLY 610 620 630 640 650 660 WMKVYQLPEA SRGARGRPIV NLLPLEANER ITAILPVREY TEGFNIFMAT ADGTVKKTAL 670 680 690 700 710 720 QEFSRPRSAG IIAINLREDD ELIGVALTNG SDETMLFSAA GKVVRFAEAA VRAMGRSASG 730 740 750 760 770 780 VRGIKLAEGD KVVSLIVPRE EGAILTVTQN GYGKRTANSE YPTKSRATQG VISIKVTERN 790 800 810 820 830 840 GPVVGAVQVD DSDQIMMITD AGTLVRTRVS EVSVVGRNTQ GVILIRTAED ENVVGLQRVA 850 860 870 EPVEEEELDS IDGSVAEGDD DIAPEVDSDD DAPDADEEE

References

[1]

"Complete sequence chromosome of Pantoea sp. At-9b." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T. Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: At-9b EMBL ADU70000.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002433 Genomic DNA. Translation: ADU70000.1.
RefSeq	YP_004116556.1. NC_014837.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADU70000; ADU70000; Pat9b_2700.
GeneID	10070141.
KEGG	pao:Pat9b_2700.
PATRIC	45319788. VBIPanSp129740_2700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076278.
KO	K02469.
Enzyme and pathway databases
BioCyc	PSP592316:GI0L-4665-MONOMER.
Family and domain databases
Gene3D	1.10.268.10. 1 hit. 3.30.1360.40. 1 hit. 3.90.199.10. 1 hit.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E6WG76_PANSA

Accession

Primary (citable) accession number: E6WG76

Entry history

Integrated into UniProtKB/TrEMBL:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)