Periplasmic nitrate reductase precursor - Pantoea sp. (strain At-9b)

Contribute

Send feedback

Read comments (?) or add your own

E6WCW7 (E6WCW7_PANSA) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 18. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase HAMAP-Rule MF_01630
EC=1.7.99.4 HAMAP-Rule MF_01630

Gene names

Name:	napA HAMAP-Rule MF_01630
Ordered Locus Names:	Pat9b_2863 EMBL ADU70163.1

Organism

Pantoea sp. (strain At-9b) [Complete proteome] [HAMAP] EMBL ADU70163.1

Taxonomic identifier

592316 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pantoea

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630 SAAS SAAS006656
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 1 molybdenum ion per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subunit structure	Interacts with NapB By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subcellular location	Periplasm By similarity HAMAP-Rule MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven By similarity. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. HAMAP-Rule MF_01630 Contains 1 4Fe-4S Mo/W bis-MGD-type domain. HAMAP-Rule MF_01630

Ontologies

Keywords
Biological process	Electron transport HAMAP-Rule MF_01630 SAAS SAAS006656 Nitrate assimilation HAMAP-Rule MF_01630 SAAS SAAS006656 Transport
Cellular component	Periplasm HAMAP-Rule MF_01630
Domain	Signal HAMAP-Rule MF_01630 EMBL ADU70163.1
Ligand	4Fe-4S HAMAP-Rule MF_01630 SAAS SAAS006656 Iron Iron-sulfur Metal-binding Molybdenum HAMAP-Rule MF_01630 SAAS SAAS006656
Molecular function	Oxidoreductase HAMAP-Rule MF_01630 SAAS SAAS006656
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

Potential EMBL ADU70163.1

Signal peptide

1 – 31

Tat-type signal By similarity HAMAP-Rule MF_01630

Chain

33 – 828

796

Potential

PRO_5000676968

Regions

Domain

39 – 95

4Fe-4S Mo/W bis-MGD-type By similarity HAMAP-Rule MF_01630

Sites

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Sequences

Sequence

Length

Mass (Da)

Tools

E6WCW7 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 468978A70182105C
Show »

FASTA

828

92,316

        10         20         30         40         50         60 
MQLSRRDFMK ANAAAAAAAA AGLVIPSVTK AATSLAAPIR WEKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
EGRVVASQGD PQAEVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMTDGRFD KNGEFTPVSW 

       130        140        150        160        170        180 
DQAFDIMEEK FKAALKANGP ESIGMFGSGQ WTLWEGYAAS KLMKAGFRSN NLDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGSNMAEMH PILWSRVTAR RLSNENVKVA 

       250        260        270        280        290        300 
VLSTYRHRSF ELADNGMVFV PQTDLVILNF IANYIIQNGK VDKTFLANHV TLRQGATDIG 

       310        320        330        340        350        360 
YGLRPSDPRQ ARAKNPDSDA STPINFDEYA KFVAEYTLEK ASAMSGVEQD QLVKLAELYA 

       370        380        390        400        410        420 
DPKINVVSYW TMGFNQHTRG VWANNLVYNL HLLTGKISRP GTGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFSHRLPAD MVVTNDKHRA LAEKLWKLPS GTIPAKPGYH AVAQDRALKD GKINVYWSMC 

       490        500        510        520        530        540 
NNNMQAGPNL TQERMPGWRD ERNFVVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWHQQVK APGEAKSDLW QLVNFSKRFR VADVWPEALL AQEPALRDKT LYDVLFANGQ 

       610        620        630        640        650        660 
VNQFPLSEVA DDQLNDDARA FGFYLQKGLF EEYASFGRGH GHDLAPFDAY HQARGLRWPV 

       670        680        690        700        710        720 
VDGKETRWRY REGHDSYVKA GEGVRFYGKP DGKATIFALP YEPPAESPDA EYDLWFCTGR 

       730        740        750        760        770        780 
VLEHWHTGTM TRRVPELHRA VPQALLYIHP LDAKARGLRR GEKVRVVSRR GEAMATVETR 

       790        800        810        820 
GRNQPPQGLV FTPFFDAGVL VNNITLDATD PLSKQTDYKK CAVKLVKI

« Hide

References

[1]

"Complete sequence chromosome of Pantoea sp. At-9b."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.
Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: At-9b EMBL ADU70163.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002433 Genomic DNA. Translation: ADU70163.1.
RefSeq	YP_004116719.1. NC_014837.1.
3D structure databases
ProteinModelPortal	E6WCW7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADU70163; ADU70163; Pat9b_2863.
GeneID	10070311.
KEGG	pao:Pat9b_2863.
PATRIC	45320128. VBIPanSp129740_2862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000031441.
KO	K02567.
Enzyme and pathway databases
BioCyc	PSP592316:GI0L-2932-MONOMER.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_4Fe-4S_dom. IPR027467. MopterinOxRdtase_cofactor_BS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E6WCW7_PANSA

Accession

Primary (citable) accession number: E6WCW7

Entry history

Integrated into UniProtKB/TrEMBL:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	May 29, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information