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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pantoea sp. (strain At-9b)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotationSAAS annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mo-bis(molybdopterin guanine dinucleotide)Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationSAAS annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotationSAAS annotation
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster.
  • [4Fe-4S] clusterUniRule annotationSAAS annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Biological processi

Electron transportUniRule annotationSAAS annotation, Nitrate assimilationUniRule annotationSAAS annotation, Transport

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, MolybdenumUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPSP592316:GI0L-2932-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Pat9b_2863Imported
OrganismiPantoea sp. (strain At-9b)Imported
Taxonomic identifieri592316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea
ProteomesiUP000001624 Componenti: Chromosome

Subcellular locationi

Periplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

PeriplasmUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 PotentialImportedAdd
BLAST
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 828796 PotentialImportedPRO_5000676968Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE6WCW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

SignalUniRule annotationImported

Phylogenomic databases

HOGENOMiHOG000031441.
KOiK02567.
OMAiREYTPKM.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E6WCW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLSRRDFMK ANAAAAAAAA AGLVIPSVTK AATSLAAPIR WEKAPCRFCG
60 70 80 90 100
TGCGVLVGTQ EGRVVASQGD PQAEVNRGLN CIKGYFLPKI MYGKDRLTQP
110 120 130 140 150
LLRMTDGRFD KNGEFTPVSW DQAFDIMEEK FKAALKANGP ESIGMFGSGQ
160 170 180 190 200
WTLWEGYAAS KLMKAGFRSN NLDPNARHCM ASAVVGFMRT FGMDEPMGCY
210 220 230 240 250
DDIEQADAFV LWGSNMAEMH PILWSRVTAR RLSNENVKVA VLSTYRHRSF
260 270 280 290 300
ELADNGMVFV PQTDLVILNF IANYIIQNGK VDKTFLANHV TLRQGATDIG
310 320 330 340 350
YGLRPSDPRQ ARAKNPDSDA STPINFDEYA KFVAEYTLEK ASAMSGVEQD
360 370 380 390 400
QLVKLAELYA DPKINVVSYW TMGFNQHTRG VWANNLVYNL HLLTGKISRP
410 420 430 440 450
GTGPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNDKHRA LAEKLWKLPS
460 470 480 490 500
GTIPAKPGYH AVAQDRALKD GKINVYWSMC NNNMQAGPNL TQERMPGWRD
510 520 530 540 550
ERNFVVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWHQQVK
560 570 580 590 600
APGEAKSDLW QLVNFSKRFR VADVWPEALL AQEPALRDKT LYDVLFANGQ
610 620 630 640 650
VNQFPLSEVA DDQLNDDARA FGFYLQKGLF EEYASFGRGH GHDLAPFDAY
660 670 680 690 700
HQARGLRWPV VDGKETRWRY REGHDSYVKA GEGVRFYGKP DGKATIFALP
710 720 730 740 750
YEPPAESPDA EYDLWFCTGR VLEHWHTGTM TRRVPELHRA VPQALLYIHP
760 770 780 790 800
LDAKARGLRR GEKVRVVSRR GEAMATVETR GRNQPPQGLV FTPFFDAGVL
810 820
VNNITLDATD PLSKQTDYKK CAVKLVKI
Length:828
Mass (Da):92,316
Last modified:March 8, 2011 - v1
Checksum:i468978A70182105C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002433 Genomic DNA. Translation: ADU70163.1.
RefSeqiWP_013510016.1. NC_014837.1.
YP_004116719.1. NC_014837.1.

Genome annotation databases

EnsemblBacteriaiADU70163; ADU70163; Pat9b_2863.
KEGGipao:Pat9b_2863.
PATRICi45320128. VBIPanSp129740_2862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002433 Genomic DNA. Translation: ADU70163.1.
RefSeqiWP_013510016.1. NC_014837.1.
YP_004116719.1. NC_014837.1.

3D structure databases

ProteinModelPortaliE6WCW7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADU70163; ADU70163; Pat9b_2863.
KEGGipao:Pat9b_2863.
PATRICi45320128. VBIPanSp129740_2862.

Phylogenomic databases

HOGENOMiHOG000031441.
KOiK02567.
OMAiREYTPKM.

Enzyme and pathway databases

BioCyciPSP592316:GI0L-2932-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence chromosome of Pantoea sp. At-9b."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.
    Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: At-9bImported.

Entry informationi

Entry nameiE6WCW7_PANSA
AccessioniPrimary (citable) accession number: E6WCW7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: April 1, 2015
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.