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E6WCW7

- E6WCW7_PANSA

UniProt

E6WCW7 - E6WCW7_PANSA

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Protein

Periplasmic nitrate reductase

Gene
napA, Pat9b_2863
Organism
Pantoea sp. (strain At-9b)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotationSAAS annotations

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotationSAAS annotations
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S) By similarityUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotations

Keywords - Biological processi

Electron transportUniRule annotationSAAS annotations, Nitrate assimilationUniRule annotationSAAS annotations, Transport

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotations, Iron, Iron-sulfur, Metal-binding, MolybdenumUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciPSP592316:GI0L-2932-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Pat9b_2863Imported
OrganismiPantoea sp. (strain At-9b)Imported
Taxonomic identifieri592316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea
ProteomesiUP000001624: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

PeriplasmUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 PredictedImportedAdd
BLAST
Signal peptidei1 – 3131Tat-type signal By similarityUniRule annotationAdd
BLAST
Chaini33 – 828796 PredictedImportedPRO_5000676968Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven By similarity.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliE6WCW7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-type By similarityUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4Fe-4S Mo/W bis-MGD-type domain.SAAS annotations

Keywords - Domaini

SignalUniRule annotationImported

Phylogenomic databases

HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E6WCW7-1 [UniParc]FASTAAdd to Basket

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MQLSRRDFMK ANAAAAAAAA AGLVIPSVTK AATSLAAPIR WEKAPCRFCG    50
TGCGVLVGTQ EGRVVASQGD PQAEVNRGLN CIKGYFLPKI MYGKDRLTQP 100
LLRMTDGRFD KNGEFTPVSW DQAFDIMEEK FKAALKANGP ESIGMFGSGQ 150
WTLWEGYAAS KLMKAGFRSN NLDPNARHCM ASAVVGFMRT FGMDEPMGCY 200
DDIEQADAFV LWGSNMAEMH PILWSRVTAR RLSNENVKVA VLSTYRHRSF 250
ELADNGMVFV PQTDLVILNF IANYIIQNGK VDKTFLANHV TLRQGATDIG 300
YGLRPSDPRQ ARAKNPDSDA STPINFDEYA KFVAEYTLEK ASAMSGVEQD 350
QLVKLAELYA DPKINVVSYW TMGFNQHTRG VWANNLVYNL HLLTGKISRP 400
GTGPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNDKHRA LAEKLWKLPS 450
GTIPAKPGYH AVAQDRALKD GKINVYWSMC NNNMQAGPNL TQERMPGWRD 500
ERNFVVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWHQQVK 550
APGEAKSDLW QLVNFSKRFR VADVWPEALL AQEPALRDKT LYDVLFANGQ 600
VNQFPLSEVA DDQLNDDARA FGFYLQKGLF EEYASFGRGH GHDLAPFDAY 650
HQARGLRWPV VDGKETRWRY REGHDSYVKA GEGVRFYGKP DGKATIFALP 700
YEPPAESPDA EYDLWFCTGR VLEHWHTGTM TRRVPELHRA VPQALLYIHP 750
LDAKARGLRR GEKVRVVSRR GEAMATVETR GRNQPPQGLV FTPFFDAGVL 800
VNNITLDATD PLSKQTDYKK CAVKLVKI 828
Length:828
Mass (Da):92,316
Last modified:March 8, 2011 - v1
Checksum:i468978A70182105C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002433 Genomic DNA. Translation: ADU70163.1.
RefSeqiWP_013510016.1. NC_014837.1.
YP_004116719.1. NC_014837.1.

Genome annotation databases

EnsemblBacteriaiADU70163; ADU70163; Pat9b_2863.
GeneIDi10070311.
KEGGipao:Pat9b_2863.
PATRICi45320128. VBIPanSp129740_2862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002433 Genomic DNA. Translation: ADU70163.1 .
RefSeqi WP_013510016.1. NC_014837.1.
YP_004116719.1. NC_014837.1.

3D structure databases

ProteinModelPortali E6WCW7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADU70163 ; ADU70163 ; Pat9b_2863 .
GeneIDi 10070311.
KEGGi pao:Pat9b_2863.
PATRICi 45320128. VBIPanSp129740_2862.

Phylogenomic databases

HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.

Enzyme and pathway databases

BioCyci PSP592316:GI0L-2932-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence chromosome of Pantoea sp. At-9b."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: At-9bImported.

Entry informationi

Entry nameiE6WCW7_PANSA
AccessioniPrimary (citable) accession number: E6WCW7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: September 3, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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