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E6WCW7

- E6WCW7_PANSA

UniProt

E6WCW7 - E6WCW7_PANSA

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pantoea sp. (strain At-9b)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (08 Mar 2011)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotationSAAS annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotationSAAS annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Electron transportUniRule annotationSAAS annotation, Nitrate assimilationUniRule annotationSAAS annotation, Transport

    Keywords - Ligandi

    4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, MolybdenumUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciPSP592316:GI0L-2932-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Pat9b_2863Imported
    OrganismiPantoea sp. (strain At-9b)Imported
    Taxonomic identifieri592316 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea
    ProteomesiUP000001624: Chromosome

    Subcellular locationi

    Periplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    PeriplasmUniRule annotation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232 PotentialImportedAdd
    BLAST
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 828796 PotentialImportedPRO_5000676968Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliE6WCW7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation
    Contains 4Fe-4S Mo/W bis-MGD-type domain.SAAS annotation

    Keywords - Domaini

    SignalUniRule annotationImported

    Phylogenomic databases

    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    E6WCW7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLSRRDFMK ANAAAAAAAA AGLVIPSVTK AATSLAAPIR WEKAPCRFCG    50
    TGCGVLVGTQ EGRVVASQGD PQAEVNRGLN CIKGYFLPKI MYGKDRLTQP 100
    LLRMTDGRFD KNGEFTPVSW DQAFDIMEEK FKAALKANGP ESIGMFGSGQ 150
    WTLWEGYAAS KLMKAGFRSN NLDPNARHCM ASAVVGFMRT FGMDEPMGCY 200
    DDIEQADAFV LWGSNMAEMH PILWSRVTAR RLSNENVKVA VLSTYRHRSF 250
    ELADNGMVFV PQTDLVILNF IANYIIQNGK VDKTFLANHV TLRQGATDIG 300
    YGLRPSDPRQ ARAKNPDSDA STPINFDEYA KFVAEYTLEK ASAMSGVEQD 350
    QLVKLAELYA DPKINVVSYW TMGFNQHTRG VWANNLVYNL HLLTGKISRP 400
    GTGPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNDKHRA LAEKLWKLPS 450
    GTIPAKPGYH AVAQDRALKD GKINVYWSMC NNNMQAGPNL TQERMPGWRD 500
    ERNFVVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWHQQVK 550
    APGEAKSDLW QLVNFSKRFR VADVWPEALL AQEPALRDKT LYDVLFANGQ 600
    VNQFPLSEVA DDQLNDDARA FGFYLQKGLF EEYASFGRGH GHDLAPFDAY 650
    HQARGLRWPV VDGKETRWRY REGHDSYVKA GEGVRFYGKP DGKATIFALP 700
    YEPPAESPDA EYDLWFCTGR VLEHWHTGTM TRRVPELHRA VPQALLYIHP 750
    LDAKARGLRR GEKVRVVSRR GEAMATVETR GRNQPPQGLV FTPFFDAGVL 800
    VNNITLDATD PLSKQTDYKK CAVKLVKI 828
    Length:828
    Mass (Da):92,316
    Last modified:March 8, 2011 - v1
    Checksum:i468978A70182105C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002433 Genomic DNA. Translation: ADU70163.1.
    RefSeqiWP_013510016.1. NC_014837.1.
    YP_004116719.1. NC_014837.1.

    Genome annotation databases

    EnsemblBacteriaiADU70163; ADU70163; Pat9b_2863.
    GeneIDi10070311.
    KEGGipao:Pat9b_2863.
    PATRICi45320128. VBIPanSp129740_2862.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002433 Genomic DNA. Translation: ADU70163.1 .
    RefSeqi WP_013510016.1. NC_014837.1.
    YP_004116719.1. NC_014837.1.

    3D structure databases

    ProteinModelPortali E6WCW7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADU70163 ; ADU70163 ; Pat9b_2863 .
    GeneIDi 10070311.
    KEGGi pao:Pat9b_2863.
    PATRICi 45320128. VBIPanSp129740_2862.

    Phylogenomic databases

    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.

    Enzyme and pathway databases

    BioCyci PSP592316:GI0L-2932-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence chromosome of Pantoea sp. At-9b."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.
      Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: At-9bImported.

    Entry informationi

    Entry nameiE6WCW7_PANSA
    AccessioniPrimary (citable) accession number: E6WCW7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 8, 2011
    Last sequence update: March 8, 2011
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3