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E6WCW7 (E6WCW7_PANSA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase HAMAP-Rule MF_01630

EC=1.7.99.4 HAMAP-Rule MF_01630
Gene names
Name:napA HAMAP-Rule MF_01630
Ordered Locus Names:Pat9b_2863 EMBL ADU70163.1
OrganismPantoea sp. (strain At-9b) [Complete proteome] [HAMAP] EMBL ADU70163.1
Taxonomic identifier592316 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630 SAAS SAAS027467

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630 SAAS SAAS027467

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630 SAAS SAAS027467

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS027467

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630 SAAS SAAS027467

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630 SAAS SAAS027467.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven By similarity. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. HAMAP-Rule MF_01630

Contains 1 4Fe-4S Mo/W bis-MGD-type domain. HAMAP-Rule MF_01630

Contains 4Fe-4S Mo/W bis-MGD-type domain. SAAS SAAS027467

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential EMBL ADU70163.1
Signal peptide1 – 3131Tat-type signal By similarity HAMAP-Rule MF_01630
Chain33 – 828796 Potential EMBL ADU70163.1
PRO_5000676968

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type By similarity HAMAP-Rule MF_01630

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630
Metal binding491Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630
Metal binding531Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630
Metal binding811Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Sequences

Sequence LengthMass (Da)Tools
E6WCW7 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 468978A70182105C

FASTA82892,316
        10         20         30         40         50         60 
MQLSRRDFMK ANAAAAAAAA AGLVIPSVTK AATSLAAPIR WEKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
EGRVVASQGD PQAEVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMTDGRFD KNGEFTPVSW 

       130        140        150        160        170        180 
DQAFDIMEEK FKAALKANGP ESIGMFGSGQ WTLWEGYAAS KLMKAGFRSN NLDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGSNMAEMH PILWSRVTAR RLSNENVKVA 

       250        260        270        280        290        300 
VLSTYRHRSF ELADNGMVFV PQTDLVILNF IANYIIQNGK VDKTFLANHV TLRQGATDIG 

       310        320        330        340        350        360 
YGLRPSDPRQ ARAKNPDSDA STPINFDEYA KFVAEYTLEK ASAMSGVEQD QLVKLAELYA 

       370        380        390        400        410        420 
DPKINVVSYW TMGFNQHTRG VWANNLVYNL HLLTGKISRP GTGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFSHRLPAD MVVTNDKHRA LAEKLWKLPS GTIPAKPGYH AVAQDRALKD GKINVYWSMC 

       490        500        510        520        530        540 
NNNMQAGPNL TQERMPGWRD ERNFVVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWHQQVK APGEAKSDLW QLVNFSKRFR VADVWPEALL AQEPALRDKT LYDVLFANGQ 

       610        620        630        640        650        660 
VNQFPLSEVA DDQLNDDARA FGFYLQKGLF EEYASFGRGH GHDLAPFDAY HQARGLRWPV 

       670        680        690        700        710        720 
VDGKETRWRY REGHDSYVKA GEGVRFYGKP DGKATIFALP YEPPAESPDA EYDLWFCTGR 

       730        740        750        760        770        780 
VLEHWHTGTM TRRVPELHRA VPQALLYIHP LDAKARGLRR GEKVRVVSRR GEAMATVETR 

       790        800        810        820 
GRNQPPQGLV FTPFFDAGVL VNNITLDATD PLSKQTDYKK CAVKLVKI 

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References

[1]"Complete sequence chromosome of Pantoea sp. At-9b."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pinto A., Currie C., Woyke T.
Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: At-9b EMBL ADU70163.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002433 Genomic DNA. Translation: ADU70163.1.
RefSeqYP_004116719.1. NC_014837.1.

3D structure databases

ProteinModelPortalE6WCW7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADU70163; ADU70163; Pat9b_2863.
GeneID10070311.
KEGGpao:Pat9b_2863.
PATRIC45320128. VBIPanSp129740_2862.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000031441.
KOK02567.
OMAHPKTRVA.

Enzyme and pathway databases

BioCycPSP592316:GI0L-2932-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6WCW7_PANSA
AccessionPrimary (citable) accession number: E6WCW7
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: June 11, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)