ID   E6VUT5_PSEA9            Unreviewed;       290 AA.
AC   E6VUT5;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   27-MAR-2024, entry version 71.
DE   RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN   OrderedLocusNames=Daes_1312 {ECO:0000313|EMBL:ADU62326.1};
OS   Pseudodesulfovibrio aespoeensis (strain ATCC 700646 / DSM 10631 / Aspo-2)
OS   (Desulfovibrio aespoeensis).
OC   Bacteria; Thermodesulfobacteriota; Desulfovibrionia; Desulfovibrionales;
OC   Desulfovibrionaceae.
OX   NCBI_TaxID=643562 {ECO:0000313|EMBL:ADU62326.1, ECO:0000313|Proteomes:UP000002191};
RN   [1] {ECO:0000313|Proteomes:UP000002191}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700646 / DSM 10631 / Aspo-2
RC   {ECO:0000313|Proteomes:UP000002191};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Chertkov O., Misra M., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA   Kyrpides N., Ivanova N., Ovchinnikova G., Pedersen K., Jagevall S.,
RA   Hazen T., Woyke T.;
RT   "Complete sequence of Desulfovibrio aespoeensis Aspo-2.";
RL   Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADU62326.1, ECO:0000313|Proteomes:UP000002191}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700646 / DSM 10631 / Aspo-2
RC   {ECO:0000313|Proteomes:UP000002191};
RX   PubMed=24874683;
RA   Pedersen K., Bengtsson A., Edlund J., Rabe L., Hazen T., Chakraborty R.,
RA   Goodwin L., Shapiro N.;
RT   "Complete Genome Sequence of the Subsurface, Mesophilic Sulfate-Reducing
RT   Bacterium Desulfovibrio aespoeensis Aspo-2.";
RL   Genome Announc. 2:e00509-14(2014).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC         methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC         Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC         Evidence={ECO:0000256|ARBA:ARBA00034420};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC         Evidence={ECO:0000256|ARBA:ARBA00034420};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974,
CC         ECO:0000256|RuleBase:RU003862};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC       {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
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DR   EMBL; CP002431; ADU62326.1; -; Genomic_DNA.
DR   RefSeq; WP_013514257.1; NC_014844.1.
DR   AlphaFoldDB; E6VUT5; -.
DR   STRING; 643562.Daes_1312; -.
DR   KEGG; das:Daes_1312; -.
DR   eggNOG; COG0685; Bacteria.
DR   HOGENOM; CLU_025841_0_0_7; -.
DR   OrthoDB; 9812555at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002191; Chromosome.
DR   GO; GO:0106312; F:methylenetetrahydrofolate reductase NADH activity; IEA:RHEA.
DR   GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00537; MTHFR; 1.
DR   Gene3D; 3.20.20.220; -; 1.
DR   InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR   InterPro; IPR003171; Mehydrof_redctse-like.
DR   PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   Pfam; PF02219; MTHFR; 1.
DR   SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW   ECO:0000256|RuleBase:RU003862};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|RuleBase:RU003862}.
SQ   SEQUENCE   290 AA;  31563 MW;  6575F576B92B3DF2 CRC64;
     MHVCDLIEKN TPFISLEFFP PKEKDAWPGF FDVVEKLKDL GPLFASVTYG AGGGTQDNTL
     EIATRMKRDH GIEPIVHLTS VGASAAKLDT FLAGLSAAGL ENVLALRGDP PKGQEDFSFD
     SQEFKHATDV IEFISRRYPN ICVGGAAYPE SHPESPSIQS DLDMVNLKVR MGAKFLVTQL
     FFDNRLYFDY VARLRAMGAD VPVVPGVLPI MNLRSAKFIL GLCGAAIPGK YLSALEKANE
     EGGDEAVYAL GIRYAIRQAQ ELIDGGAPGV HLYTLNRAEA CLEIGKNLKI
//