Isocitrate dehydrogenase [NADP] - Clostridium thermocellum (strain DSM 1313 / LMG 6656 / LQ8)

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E6ULW2 (E6ULW2_CLOTL) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 19. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Ordered Locus Names:

Clo1313_1944

Organism

Clostridium thermocellum (strain DSM 1313 / LMG 6656 / LQ8) [Complete proteome] [HAMAP] EMBL ADU74991.1

Taxonomic identifier

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 PIRSR PIRSR000108-3 Manganese PIRNR PIRNR000108 PIRSR PIRSR000108-3 Metal-binding PIRNR PIRNR000108 PIRSR PIRSR000108-3 NADP PIRNR PIRNR000108
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444
Technical term	3D-structure PDB 4AOY Complete proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

7

Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding

1

Magnesium or manganese By similarity PIRSR PIRSR000108-3

Metal binding

1

Magnesium or manganese By similarity PIRSR PIRSR000108-3

Binding site

1

Substrate By similarity PIRSR PIRSR000108-2

Binding site

1

Substrate By similarity PIRSR PIRSR000108-2

Binding site

1

Substrate By similarity PIRSR PIRSR000108-2

Site

1

Critical for catalysis By similarity PIRSR PIRSR000108-1

Site

1

Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence

Length

Mass (Da)

Tools

E6ULW2 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 5A55781A7AC5B42E
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402

45,782

        10         20         30         40         50         60 
MSKIKMKVPL VEMDGDEMTR IIWRLIKENL LEPYIELNTE YYDLGLENRD KTEDQVTIDA 

        70         80         90        100        110        120 
ARAIQKYGVG VKCATITPNA QRVEEYNLKK MWKSPNGTIR AILDGTVFRA PIVVNSIKPF 

       130        140        150        160        170        180 
VKGWKKPISI ARHAYGDVYK NVEYYVPSAG KAELVFTSEN GEVSRQTIHE FDGPGVIMGM 

       190        200        210        220        230        240 
HNTDKSIRSF ARACFNYALD MNQDLWFSTK DTISKTYDHR FKDIFQEIYE NEYKEKFEAK 

       250        260        270        280        290        300 
NLQYFYTLID DAVARIIRSE GGMVWACKNY DGDVMSDMVA SAFGSLAMMT SVLVSPDGKY 

       310        320        330        340        350        360 
EFEAAHGTVT RHYYKHLKGE ETSTNSMATI FAWTGALKKR GELDGIKELV DFATKLEQAS 

       370        380        390        400 
VQTIENGVMT KDLASLSEVP EKKIVNTEDF LKEIRKTFEG MA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the cellulolytic thermophile Clostridium thermocellum DSM1313." Feinberg L., Foden J., Barrett T., Davenport K.W., Bruce D., Detter C., Tapia R., Han C., Lapidus A., Lucas S., Cheng J.F., Pitluck S., Woyke T., Ivanova N., Mikhailova N., Land M., Hauser L., Argyros D.A. , Goodwin L., Hogsett D., Caiazza N. J. Bacteriol. 193:2906-2907(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 1313 / LMG 6656 / LQ8.
[2]	"The Complex Structures of Isocitrate Dehydrogenase from Clostridium Thermocellum and Desulfotalea Psychrophila, Support a New Active Site Locking Mechanism." Leiros H.-K.S., Fedoy A.-E., Leiros I., Steen I.H. Submitted (MAR-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP002416 Genomic DNA. Translation: ADU74991.1.

RefSeq

YP_005688442.1. NC_017304.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AOY	X-ray	2.35	A/B/C/D	1-402	[»]

ProteinModelPortal

SMR

E6ULW2. Positions 3-401.

ModBase

Protocols and materials databases

StructuralBiologyKnowledgebase

Genome annotation databases

EnsemblBacteria

ADU74991; ADU74991; Clo1313_1944.

GeneID

KEGG

ctx:Clo1313_1944.

PATRIC

45478813. VBICloThe140364_2116.

Organism-specific databases

CMR

Phylogenomic databases

KO

OMA

Family and domain databases

Gene3D

3.40.718.10. 1 hit.

InterPro

IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]

PANTHER

PTHR11822. PTHR11822. 1 hit.

Pfam

PF00180. Iso_dh. 1 hit.
[Graphical view]

PIRSF

PIRSF000108. IDH_NADP. 1 hit.

TIGRFAMs

TIGR00127. nadp_idh_euk. 1 hit.

PROSITE

PS00470. IDH_IMDH. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

E6ULW2_CLOTL

Accession

Primary (citable) accession number: E6ULW2

Entry history

Integrated into UniProtKB/TrEMBL:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info