E6U338 (E6U338_ETHHY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
EC=2.4.1.18 HAMAP-Rule MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP-Rule MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP-Rule MF_00685
Glycogen branching enzyme HAMAP-Rule MF_00685

Gene names

Name:	glgB HAMAP-Rule MF_00685
Ordered Locus Names:	Ethha_1992

Organism

Ethanoligenens harbinense (strain DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3) [Complete proteome] [HAMAP] EMBL ADU27510.1

Taxonomic identifier

663278 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ethanoligenens › Ethanoligenens harbinense

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685 SAAS SAAS006048
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP-Rule MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP-Rule MF_00685 Glycogen metabolism HAMAP-Rule MF_00685
Molecular function	Glycosyltransferase HAMAP-Rule MF_00685 SAAS SAAS006048 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	313	1	Nucleophile By similarity HAMAP-Rule MF_00685
	Active site	366	1	Proton donor By similarity HAMAP-Rule MF_00685

Sequences

	Sequence		Length	Mass (Da)
	E6U338 [UniParc]. Last modified March 8, 2011. Version 1. Checksum: B7417F916BBDD377	FASTA	634	73,621
	10 20 30 40 50 60 MYTNAPKKTG REQELQCFHE SVYPLSFEYF GGHSSHRSKK SGVVFRVWAP KAVSVSIVGD 70 80 90 100 110 120 FNNWDASKNP MISVDDQGVW EGFVPGIKSF DIYKYQIETA DGKTLLKSDP YATHMETRPD 130 140 150 160 170 180 TASRYYNLSG YHWKDQEWQK KKEKKSAYDV PMNIYELHAG SWKKYPDGNP FEYRKLAEEL 190 200 210 220 230 240 IPYLQKMGYT HVELMPIMEY PLDNSWGYQV TGYFAPTSRY GSPRDFMAFV DYCHQADIGV 250 260 270 280 290 300 IMDWVPAHFP KDAHGLYRFD GDCCYEYTDP LKQEHKSWGT CVFDYSKSEV RSFLVSNAIF 310 320 330 340 350 360 WLQKYHIDGL RVDAVASMLY LDYDRKNGEW RPNQYGGHEN LEAVSFLQEL NREVFSRFPN 370 380 390 400 410 420 ALMIAEESTA WPLVTKPVDV GGLGFNFKWN MGWMNDMLDY VSTDPYFRKD HHKDITFSFH 430 440 450 460 470 480 YAFSENYILP ISHDEVVHGK RSLLEKMPGD KAEQFAGVRV FLAYMIAHPG KKLLFMGSEF 490 500 510 520 530 540 GQVHEWDCHD ALQWQLLGEK GHQNLQDFTA ALNHFYLDQS PLWQNDFSWE GFQWISYDDN 550 560 570 580 590 600 LQNIIAFRRI DRKGEELVCL FNFAPVKRDD YRIGIPAAGY YQEAFNTDAE QFGGTGESNK 610 620 630 EPVPTEKIPL HGYQQSIALT VPPMAALFLK HQPK

References

[1]

"Complete sequence of Ethanoligenens harbinense YUAN-3." Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Misra M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N. , Wang A., Mouttaki H., He Z., Zhou J., Hemme C.L., Woyke T. Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002400 Genomic DNA. Translation: ADU27510.1.
RefSeq	YP_004092241.1. NC_014828.1.
3D structure databases
ProteinModelPortal	E6U338.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADU27510; ADU27510; Ethha_1992.
GeneID	10092972.
KEGG	eha:Ethha_1992.
PATRIC	45211264. VBIEthHar145175_2168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000283037.
KO	K00700.
Enzyme and pathway databases
BioCyc	EHAR663278:GIWT-2033-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E6U338_ETHHY

Accession

Primary (citable) accession number: E6U338

Entry history

Integrated into UniProtKB/TrEMBL:	March 8, 2011
Last sequence update:	March 8, 2011
Last modified:	May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)