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E6U1T2 (E6U1T2_BACCJ) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Bcell_3337 EMBL ADU31579.1
OrganismBacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4) [Complete proteome] [HAMAP] EMBL ADU31579.1
Taxonomic identifier649639 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain154 – 370217ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding187 – 24256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3271Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3411Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3411Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3431Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E6U1T2 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: 6470F1D94D846A52

FASTA40444,691
        10         20         30         40         50         60 
MNKEGVRMRT RVAVIFGGMS VEHEVSVISA LQAIQNMDES RYEIIPVYIS KEGAWYTGDT 

        70         80         90        100        110        120 
LLDIEEYKNL KGLLAKSQRI QLLREGDNRV IINKEPKPKF GKAYINEIDV AFPIVHGTFA 

       130        140        150        160        170        180 
EDGVLQGYLE LLGLPYVGCD VVSSSTGMDK VIMKNVYRDS GLPILDYVWF YSTKWNEDQD 

       190        200        210        220        230        240 
ELVKKVEGKL QYPVIVKPAN LGSSVGISKA ANVDELEEAV ELASQFANKI VIEKMVQNMT 

       250        260        270        280        290        300 
EINCSVLGDY EAAEASVCEQ VLGNDEILSY EDKYAGGGSK GGGASKGMES TNRIIPADIS 

       310        320        330        340        350        360 
EEKTKEIQEL AVAAFQVLGC SGVSRIDFLI DNDTNNVFIN EINTIPGSLS FYLWEPTGKP 

       370        380        390        400 
YKELTNDLIQ LALKRTRERE QLMFSIDSNL FSLGKLSGGK GSKN 

« Hide

References

[1]"Complete sequence of Bacillus cellulosilyticus DSM 2522."
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Brumm P., Mead D., Woyke T.
Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002394 Genomic DNA. Translation: ADU31579.1.
RefSeqYP_004096310.1. NC_014829.1.

3D structure databases

ProteinModelPortalE6U1T2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADU31579; ADU31579; Bcell_3337.
GeneID10089917.
KEGGbco:Bcell_3337.
PATRIC45162738. VBIBacCel7049_3433.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.

Enzyme and pathway databases

BioCycBCEL649639:GHTT-3425-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6U1T2_BACCJ
AccessionPrimary (citable) accession number: E6U1T2
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: February 19, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)