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E6U0M2

- E6U0M2_BACCJ

UniProt

E6U0M2 - E6U0M2_BACCJ

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Bacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Magnesium.UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Binding sitei154 – 1541Allosteric activator ADPUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
Binding sitei211 – 2111Allosteric activator ADPUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Binding sitei243 – 2431Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPUniRule annotation
Nucleotide bindingi102 – 1054ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEL649639:GHTT-3313-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Bcell_3225Imported
OrganismiBacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)Imported
Taxonomic identifieri649639 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001401: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE6U0M2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni125 – 1273Substrate bindingUniRule annotation
Regioni169 – 1713Substrate bindingUniRule annotation
Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
Regioni213 – 2153Allosteric activator ADP bindingUniRule annotation
Regioni249 – 2524Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E6U0M2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRIGVLTSG GDSPGMNAAI RAVVRKAIFH NLEVYGIYYG YAGLISGDIK
60 70 80 90 100
KLEIGSVGDI IHRGGTMLYT ARCEEFKTLE GQKKGIEQLK KFGIEALVVI
110 120 130 140 150
GGDGSFQGAK KLTEHGFPTI GVPGTIDNDI PGTDFTIGFD TALNTVIDAV
160 170 180 190 200
DKIRDTATSH ERTYVIEVMG RDAGDIALWA GLADGAESIL IPEDDFSMDD
210 220 230 240 250
IIDRLEKGHK RGKKHSIIVV AEGCGSGVEI GKTIEERTKL DTRVTVLGHI
260 270 280 290 300
QRGGSPTASD RVLASRLGAY AVELLLDGEA GKMVGIEKNQ LVSHDIDEAL
310
SRKHTIDLNM YKLSKELSI
Length:319
Mass (Da):34,353
Last modified:March 8, 2011 - v1
Checksum:iA5308D9387DB66E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002394 Genomic DNA. Translation: ADU31467.1.
RefSeqiWP_013489798.1. NC_014829.1.
YP_004096198.1. NC_014829.1.

Genome annotation databases

EnsemblBacteriaiADU31467; ADU31467; Bcell_3225.
GeneIDi10089805.
KEGGibco:Bcell_3225.
PATRICi45162508. VBIBacCel7049_3319.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002394 Genomic DNA. Translation: ADU31467.1 .
RefSeqi WP_013489798.1. NC_014829.1.
YP_004096198.1. NC_014829.1.

3D structure databases

ProteinModelPortali E6U0M2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADU31467 ; ADU31467 ; Bcell_3225 .
GeneIDi 10089805.
KEGGi bco:Bcell_3225.
PATRICi 45162508. VBIBacCel7049_3319.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci BCEL649639:GHTT-3313-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Bacillus cellulosilyticus DSM 2522."
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Brumm P., Mead D., Woyke T.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4Imported.

Entry informationi

Entry nameiE6U0M2_BACCJ
AccessioniPrimary (citable) accession number: E6U0M2
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: October 29, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3