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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Bacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactori

FADUniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

FADUniRule annotationSAAS annotation, Flavoprotein

Enzyme and pathway databases

BioCyciBCEL649639:GHTT-2592-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotationSAAS annotation (EC:2.1.1.74UniRule annotationSAAS annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Bcell_2511Imported
OrganismiBacillus cellulosilyticus (strain ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4)Imported
Taxonomic identifieri649639 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001401 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE6TSX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E6TSX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQHVNVIGA GLAGSEAAWQ IAKRGIPVHL YEMRPKKQTP AHHTDKFAEL
60 70 80 90 100
VCSNSLRANT LTNAVGVLKE EMRILDSVII QSADDCSVPA GGALAVDRHE
110 120 130 140 150
FAALVTERVK NHELVTVFED EVTEIPEGPT IICTGPLTSE SLSHRLKELT
160 170 180 190 200
GEEYLYFYDA AAPIIDADSI DRNKVYLKSR YDKGEAAYLN CPMTEEEFNT
210 220 230 240 250
FYDALIEAET VPVKEFEKEM FFEGCMPVEV MAKRGRKTLL FGPMKPVGLE
260 270 280 290 300
DPKTNKRPFA VVQLRQDNSS GTLFNIVGFQ THMKWGPQKE VIQLIPGLEN
310 320 330 340 350
AEIVRFGVMH RNTFINSPNL LKPTYQYRDR DDLFFAGQMT GVEGYVESAA
360 370 380 390 400
AGLVAGINAS RLIQEKETVT FPNETVIGSM AQYITTANHK NFQPMNANFG
410 420 430
LLPPFEQRIK NKQERNEKHA NRALETIQNF VKKM
Length:434
Mass (Da):48,678
Last modified:March 8, 2011 - v1
Checksum:i7D1F584A608BDA37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002394 Genomic DNA. Translation: ADU30768.1.
RefSeqiWP_013489102.1. NC_014829.1.
YP_004095499.1. NC_014829.1.

Genome annotation databases

EnsemblBacteriaiADU30768; ADU30768; Bcell_2511.
KEGGibco:Bcell_2511.
PATRICi45161012. VBIBacCel7049_2580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002394 Genomic DNA. Translation: ADU30768.1.
RefSeqiWP_013489102.1. NC_014829.1.
YP_004095499.1. NC_014829.1.

3D structure databases

ProteinModelPortaliE6TSX6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADU30768; ADU30768; Bcell_2511.
KEGGibco:Bcell_2511.
PATRICi45161012. VBIBacCel7049_2580.

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Enzyme and pathway databases

BioCyciBCEL649639:GHTT-2592-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Bacillus cellulosilyticus DSM 2522."
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Brumm P., Mead D., Woyke T.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 21833 / DSM 2522 / FERM P-1141 / JCM 9156 / N-4Imported.

Entry informationi

Entry nameiE6TSX6_BACCJ
AccessioniPrimary (citable) accession number: E6TSX6
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: April 29, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.