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Protein

Biotin biosynthesis bifunctional protein BioAB

Gene

bioB

Organism
Bacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism, and the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA).By similarity

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity
  • pyridoxal 5'-phosphateBy similarity

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin biosynthesis bifunctional protein BioAB (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Pathwayi: biotin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).
Proteins known to be involved in this subpathway in this organism are:
  1. Biotin biosynthesis bifunctional protein BioAB (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi66Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi69Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi106Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi138Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi198Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi268Iron-sulfur 2 (2Fe-2S)By similarity1
Sitei333Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity1
Binding sitei3687-keto-8-aminopelargonic acidBy similarity1
Binding sitei4617-keto-8-aminopelargonic acidBy similarity1
Binding sitei562Pyridoxal phosphateBy similarity1
Binding sitei5917-keto-8-aminopelargonic acidBy similarity1
Binding sitei6247-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity1
Binding sitei7107-keto-8-aminopelargonic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Multifunctional enzyme, Transferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBHEL693979:G1GQY-2119-MONOMER
UniPathwayiUPA00078; UER00160
UPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin biosynthesis bifunctional protein BioAB
Including the following 2 domains:
Biotin synthase BioB (EC:2.8.1.6)
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
7,8-diaminononanoate synthase
Short name:
DANS
Diaminopelargonic acid synthase
Gene namesi
Name:bioB
Ordered Locus Names:Bache_2094
OrganismiBacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108)
Taxonomic identifieri693979 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000008630 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004111341 – 744Biotin biosynthesis bifunctional protein BioABAdd BLAST744

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei591N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiE6SRG2

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi693979.Bache_2094

Structurei

3D structure databases

ProteinModelPortaliE6SRG2
SMRiE6SRG2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni428 – 429Pyridoxal phosphate bindingBy similarity2
Regioni625 – 626Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

In the N-terminal section; belongs to the radical SAM superfamily. Biotin synthase family.Curated
In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C8Y Bacteria
COG0161 LUCA
COG0502 LUCA
HOGENOMiHOG000138865
KOiK00833
OMAiKWCAQSS
OrthoDBiPOG091H01IB

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.20.20.70, 1 hit
3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00834 BioA, 1 hit
MF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005814 Aminotrans_3
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR005815 BioA
IPR002684 Biotin_synth/BioAB
IPR006638 Elp3/MiaB/NifB
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR007197 rSAM
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00508 bioA, 1 hit
TIGR00433 bioB, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

E6SRG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVNELKEQV LEGNFISREE AEWLAAQSDK EALYEAAHEI TRTLASEEFD
60 70 80 90 100
MCSIINAKSG RCPENCKWCA QSSHYKTKAD VYDLVDKEEC LRHAKYNEEQ
110 120 130 140 150
GVARFSLVTS GRKPSGKNME KLCEAARHMR RHSSIQLCAS LGLLNEEEML
160 170 180 190 200
ALHDAGITRY HCNLETAPSY FSNLCSTHTQ AEKIRTLKAA RNAGMDICSG
210 220 230 240 250
GIIGMGESME QRIEFAFTLK DMEVQSIPIN LLSPIPGTPL ERQEPLNEEE
260 270 280 290 300
ILTTIALFRF INPRAFLRFA GGRSQLSTEA VRKALHIGIN SAIVGDLLTT
310 320 330 340 350
IGSKVSEDKT LIEEAGYRFS DSQFDREHLW HPYTSTTDPL PVYKVEQAEG
360 370 380 390 400
ATITLESGQT LIEGMSSWWC AIHGYNNPVL NHAATEQIGK MSHVMFGGLT
410 420 430 440 450
HEPAIELGKL LLPLVPPSMQ KIFYADSGSV AVEVALKMAV QYWYGKGKEK
460 470 480 490 500
KNNFVTIRSG YHGDTWNAMS VCDPVTGMHS LFGSSLPIRY FAPQPRSRYD
510 520 530 540 550
GDWDAGDSME LQNIIEQHHE ELAALILEPI VQGAGGMWFY HPQYLREAAR
560 570 580 590 600
LCKQYGLLLI FDEIATGFGR TGKLFAWEHA GTEPDIMCIG KALTGGYMTL
610 620 630 640 650
SAVLTTNEVA DAISNHSPGV FMHGPTFMGN PLACAVACAS VRLLTSPVYD
660 670 680 690 700
WQGKVNRISM QLREELAPAR QLPQVKDVRI LGAIGVIEVT ENVDMAWMQR
710 720 730 740
RFVEEGIWVR PFGKLVYLMP PFIIEPEQLT KLTSGLIKII KEML
Length:744
Mass (Da):82,983
Last modified:March 8, 2011 - v1
Checksum:i6D94D64153D41FC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002352 Genomic DNA Translation: ADV44065.1
RefSeqiWP_013547658.1, NC_014933.1

Genome annotation databases

EnsemblBacteriaiADV44065; ADV44065; Bache_2094
KEGGibhl:Bache_2094
PATRICifig|693979.3.peg.2204

Similar proteinsi

Entry informationi

Entry nameiBIOAB_BACT6
AccessioniPrimary (citable) accession number: E6SRG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: March 8, 2011
Last modified: May 23, 2018
This is version 42 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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