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E6SRG2

- BIOAB_BACT6

UniProt

E6SRG2 - BIOAB_BACT6

Protein

Biotin biosynthesis bifunctional protein BioAB

Gene

bioB

Organism
Bacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (08 Mar 2011)
      Previous versions | rss
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    Functioni

    Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism, and the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA).By similarity

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.
    S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.By similarity
    Pyridoxal phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi106 – 1061Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi268 – 2681Iron-sulfur 2 (2Fe-2S)By similarity
    Sitei333 – 3331Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity
    Binding sitei368 – 36817-keto-8-aminopelargonic acidBy similarity
    Binding sitei461 – 46117-keto-8-aminopelargonic acidBy similarity
    Binding sitei562 – 5621Pyridoxal phosphateBy similarity
    Binding sitei591 – 59117-keto-8-aminopelargonic acidBy similarity
    Binding sitei624 – 62417-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity
    Binding sitei710 – 71017-keto-8-aminopelargonic acidBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: UniProtKB-EC
    4. biotin synthase activity Source: UniProtKB-EC
    5. metal ion binding Source: UniProtKB-KW
    6. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBHEL693979:GHID-2149-MONOMER.
    UniPathwayiUPA00078; UER00160.
    UPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin biosynthesis bifunctional protein BioAB
    Including the following 2 domains:
    Biotin synthase BioB (EC:2.8.1.6)
    Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA (EC:2.6.1.62)
    Alternative name(s):
    7,8-diamino-pelargonic acid aminotransferase
    Short name:
    DAPA AT
    Short name:
    DAPA aminotransferase
    7,8-diaminononanoate synthase
    Short name:
    DANS
    Diaminopelargonic acid synthase
    Gene namesi
    Name:bioB
    Ordered Locus Names:Bache_2094
    OrganismiBacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108)
    Taxonomic identifieri693979 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000008630: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744Biotin biosynthesis bifunctional protein BioABPRO_0000411134Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei591 – 5911N6-(pyridoxal phosphate)lysineBy similarity

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliE6SRG2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni428 – 4292Pyridoxal phosphate bindingBy similarity
    Regioni625 – 6262Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the radical SAM superfamily. Biotin synthase family.Curated
    In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

    Phylogenomic databases

    HOGENOMiHOG000138865.
    KOiK00833.
    OMAiKWCAQSS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPiMF_00834. BioA.
    MF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR005814. Aminotrans_3.
    IPR010722. BATS_dom.
    IPR005815. BioA.
    IPR002684. Biotin_synth/BioAB.
    IPR006638. Elp3/MiaB/NifB.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00508. bioA. 1 hit.
    TIGR00433. bioB. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E6SRG2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVNELKEQV LEGNFISREE AEWLAAQSDK EALYEAAHEI TRTLASEEFD    50
    MCSIINAKSG RCPENCKWCA QSSHYKTKAD VYDLVDKEEC LRHAKYNEEQ 100
    GVARFSLVTS GRKPSGKNME KLCEAARHMR RHSSIQLCAS LGLLNEEEML 150
    ALHDAGITRY HCNLETAPSY FSNLCSTHTQ AEKIRTLKAA RNAGMDICSG 200
    GIIGMGESME QRIEFAFTLK DMEVQSIPIN LLSPIPGTPL ERQEPLNEEE 250
    ILTTIALFRF INPRAFLRFA GGRSQLSTEA VRKALHIGIN SAIVGDLLTT 300
    IGSKVSEDKT LIEEAGYRFS DSQFDREHLW HPYTSTTDPL PVYKVEQAEG 350
    ATITLESGQT LIEGMSSWWC AIHGYNNPVL NHAATEQIGK MSHVMFGGLT 400
    HEPAIELGKL LLPLVPPSMQ KIFYADSGSV AVEVALKMAV QYWYGKGKEK 450
    KNNFVTIRSG YHGDTWNAMS VCDPVTGMHS LFGSSLPIRY FAPQPRSRYD 500
    GDWDAGDSME LQNIIEQHHE ELAALILEPI VQGAGGMWFY HPQYLREAAR 550
    LCKQYGLLLI FDEIATGFGR TGKLFAWEHA GTEPDIMCIG KALTGGYMTL 600
    SAVLTTNEVA DAISNHSPGV FMHGPTFMGN PLACAVACAS VRLLTSPVYD 650
    WQGKVNRISM QLREELAPAR QLPQVKDVRI LGAIGVIEVT ENVDMAWMQR 700
    RFVEEGIWVR PFGKLVYLMP PFIIEPEQLT KLTSGLIKII KEML 744
    Length:744
    Mass (Da):82,983
    Last modified:March 8, 2011 - v1
    Checksum:i6D94D64153D41FC0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002352 Genomic DNA. Translation: ADV44065.1.
    RefSeqiWP_013547658.1. NC_014933.1.
    YP_004161651.1. NC_014933.1.

    Genome annotation databases

    EnsemblBacteriaiADV44065; ADV44065; Bache_2094.
    GeneIDi10140872.
    KEGGibhl:Bache_2094.
    PATRICi45174812. VBIBacHel147569_2204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002352 Genomic DNA. Translation: ADV44065.1 .
    RefSeqi WP_013547658.1. NC_014933.1.
    YP_004161651.1. NC_014933.1.

    3D structure databases

    ProteinModelPortali E6SRG2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADV44065 ; ADV44065 ; Bache_2094 .
    GeneIDi 10140872.
    KEGGi bhl:Bache_2094.
    PATRICi 45174812. VBIBacHel147569_2204.

    Phylogenomic databases

    HOGENOMi HOG000138865.
    KOi K00833.
    OMAi KWCAQSS.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00160 .
    UPA00078 ; UER00162 .
    BioCyci BHEL693979:GHID-2149-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPi MF_00834. BioA.
    MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR005814. Aminotrans_3.
    IPR010722. BATS_dom.
    IPR005815. BioA.
    IPR002684. Biotin_synth/BioAB.
    IPR006638. Elp3/MiaB/NifB.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00508. bioA. 1 hit.
    TIGR00433. bioB. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108.

    Entry informationi

    Entry nameiBIOAB_BACT6
    AccessioniPrimary (citable) accession number: E6SRG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: March 8, 2011
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3