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E6S985

- E6S985_INTC7

UniProt

E6S985 - E6S985_INTC7

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Note: Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei313 – 3131Coenzyme AUniRule annotation
Binding sitei502 – 5021ATPUniRule annotation
Binding sitei517 – 5171ATPUniRule annotation
Binding sitei525 – 5251Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei528 – 5281ATPUniRule annotation
Metal bindingi539 – 5391Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi541 – 5411Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi544 – 5441Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi389 – 3913ATPUniRule annotation
Nucleotide bindingi413 – 4186ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciICAL710696:GH9U-529-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Intca_0516Imported
OrganismiIntrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)Imported
Taxonomic identifieri710696 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeIntrasporangiaceaeIntrasporangium
ProteomesiUP000008914: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei618 – 6181N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliE6S985.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 1964Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229981.
KOiK01895.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E6S985-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSETLENLLQ ETRTFPPPPE FASRANGTAE LYEKADADHE GFWAEQARTY
60 70 80 90 100
VSWSKDFTQT LQWDTPFAKW FPDGELNACY NAVDRHVEAG NGDRVAIHWI
110 120 130 140 150
GEPEGDTREI TYSDLHSSVQ RAANALQDIG VRKGDTVAIY LPMIPEAAVA
160 170 180 190 200
MLACARIGAP HSVVFGGFSA EALHSRIDDA GAKVVITSDG GYRRGSASPL
210 220 230 240 250
KPAVDAALDH GDTSVEKVLV VKRTGQETPW TEGRDLWWDE VLEQAAPTHE
260 270 280 290 300
APAHESEHPL FILYTSGTTG KPKGIFHTTG GYLTQCAYTN AVVHDVHPES
310 320 330 340 350
DVYWCTADIG WVTGHSYIVY GPLTLGATQV MYEGTPDTPH QGRFWEIVEQ
360 370 380 390 400
KKVTILYTAP TAIRTFMKWG DDIPGKFDLS SLRLLGSVGE PINPEAWMWY
410 420 430 440 450
RRVIGGDRCP IVDTWWQTET GAIMVSPLPG VTATKPGSAQ RAIPGIGAEV
460 470 480 490 500
VDDMAQPVPN GHGGYLVLTK PWPAMLRGIW GDPERYKETY WSRFEGLYFA
510 520 530 540 550
GDGAKKDDDG DIWLLGRVDD VMNVSGHRLS TTEIESALVS HPKVAEAAVV
560 570 580 590 600
GATDETTGQA VVAFVILRSE AVAEADEPGE GADIVAELRN HVAKEIGPIA
610 620 630 640 650
KPRSIMIVPE LPKTRSGKIM RRLLRDVAEN RQVGDVTTLA DSSVMELIKT

GLNKGDGD
Length:658
Mass (Da):71,897
Last modified:March 8, 2011 - v1
Checksum:i678660EA24A7E85D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002343 Genomic DNA. Translation: ADU47061.1.
RefSeqiWP_013491382.1. NC_014830.1.
YP_004097788.1. NC_014830.1.

Genome annotation databases

EnsemblBacteriaiADU47061; ADU47061; Intca_0516.
GeneIDi10083310.
KEGGiica:Intca_0516.
PATRICi45242428. VBIIntCal153415_0517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002343 Genomic DNA. Translation: ADU47061.1 .
RefSeqi WP_013491382.1. NC_014830.1.
YP_004097788.1. NC_014830.1.

3D structure databases

ProteinModelPortali E6S985.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADU47061 ; ADU47061 ; Intca_0516 .
GeneIDi 10083310.
KEGGi ica:Intca_0516.
PATRICi 45242428. VBIIntCal153415_0517.

Phylogenomic databases

HOGENOMi HOG000229981.
KOi K01895.

Enzyme and pathway databases

BioCyci ICAL710696:GH9U-529-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIPImported.

Entry informationi

Entry nameiE6S985_INTC7
AccessioniPrimary (citable) accession number: E6S985
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: November 26, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3