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E6S985

- E6S985_INTC7

UniProt

E6S985 - E6S985_INTC7

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Protein
Acetyl-coenzyme A synthetase
Gene
acsA, Intca_0516
Organism
Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA By similarity.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei313 – 3131Coenzyme A By similarityUniRule annotation
Binding sitei389 – 3891Substrate; via nitrogen amide By similarityUniRule annotation
Binding sitei502 – 5021Substrate By similarityUniRule annotation
Binding sitei517 – 5171Substrate By similarityUniRule annotation
Active sitei519 – 5191 By similarityUniRule annotation
Binding sitei525 – 5251Coenzyme A By similarityUniRule annotation
Binding sitei528 – 5281Substrate By similarityUniRule annotation
Metal bindingi539 – 5391Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi541 – 5411Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi544 – 5441Magnesium; via carbonyl oxygen By similarityUniRule annotation
Binding sitei593 – 5931Coenzyme A By similarityUniRule annotation

GO - Molecular functioni

  1. AMP binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciICAL710696:GH9U-529-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Intca_0516Imported
OrganismiIntrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)Imported
Taxonomic identifieri710696 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeIntrasporangiaceaeIntrasporangium
ProteomesiUP000008914: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei618 – 6181N6-acetyllysine By similarityUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliE6S985.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 4186Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000229981.
KOiK01895.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E6S985-1 [UniParc]FASTAAdd to Basket

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MSETLENLLQ ETRTFPPPPE FASRANGTAE LYEKADADHE GFWAEQARTY    50
VSWSKDFTQT LQWDTPFAKW FPDGELNACY NAVDRHVEAG NGDRVAIHWI 100
GEPEGDTREI TYSDLHSSVQ RAANALQDIG VRKGDTVAIY LPMIPEAAVA 150
MLACARIGAP HSVVFGGFSA EALHSRIDDA GAKVVITSDG GYRRGSASPL 200
KPAVDAALDH GDTSVEKVLV VKRTGQETPW TEGRDLWWDE VLEQAAPTHE 250
APAHESEHPL FILYTSGTTG KPKGIFHTTG GYLTQCAYTN AVVHDVHPES 300
DVYWCTADIG WVTGHSYIVY GPLTLGATQV MYEGTPDTPH QGRFWEIVEQ 350
KKVTILYTAP TAIRTFMKWG DDIPGKFDLS SLRLLGSVGE PINPEAWMWY 400
RRVIGGDRCP IVDTWWQTET GAIMVSPLPG VTATKPGSAQ RAIPGIGAEV 450
VDDMAQPVPN GHGGYLVLTK PWPAMLRGIW GDPERYKETY WSRFEGLYFA 500
GDGAKKDDDG DIWLLGRVDD VMNVSGHRLS TTEIESALVS HPKVAEAAVV 550
GATDETTGQA VVAFVILRSE AVAEADEPGE GADIVAELRN HVAKEIGPIA 600
KPRSIMIVPE LPKTRSGKIM RRLLRDVAEN RQVGDVTTLA DSSVMELIKT 650
GLNKGDGD 658
Length:658
Mass (Da):71,897
Last modified:March 8, 2011 - v1
Checksum:i678660EA24A7E85D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002343 Genomic DNA. Translation: ADU47061.1.
RefSeqiYP_004097788.1. NC_014830.1.

Genome annotation databases

EnsemblBacteriaiADU47061; ADU47061; Intca_0516.
GeneIDi10083310.
KEGGiica:Intca_0516.
PATRICi45242428. VBIIntCal153415_0517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002343 Genomic DNA. Translation: ADU47061.1 .
RefSeqi YP_004097788.1. NC_014830.1.

3D structure databases

ProteinModelPortali E6S985.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADU47061 ; ADU47061 ; Intca_0516 .
GeneIDi 10083310.
KEGGi ica:Intca_0516.
PATRICi 45242428. VBIIntCal153415_0517.

Phylogenomic databases

HOGENOMi HOG000229981.
KOi K01895.

Enzyme and pathway databases

BioCyci ICAL710696:GH9U-529-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP.

Entry informationi

Entry nameiE6S985_INTC7
AccessioniPrimary (citable) accession number: E6S985
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: June 11, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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