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E6S985

- E6S985_INTC7

UniProt

E6S985 - E6S985_INTC7

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (08 Mar 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei313 – 3131Coenzyme AUniRule annotation
    Binding sitei389 – 3891Substrate; via nitrogen amideUniRule annotation
    Binding sitei502 – 5021SubstrateUniRule annotation
    Binding sitei517 – 5171SubstrateUniRule annotation
    Active sitei519 – 5191UniRule annotation
    Binding sitei525 – 5251Coenzyme AUniRule annotation
    Binding sitei528 – 5281SubstrateUniRule annotation
    Metal bindingi539 – 5391Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi541 – 5411Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi544 – 5441Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei593 – 5931Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciICAL710696:GH9U-529-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:Intca_0516Imported
    OrganismiIntrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)Imported
    Taxonomic identifieri710696 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeIntrasporangiaceaeIntrasporangium
    ProteomesiUP000008914: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei618 – 6181N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliE6S985.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni413 – 4186Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000229981.
    KOiK01895.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E6S985-1 [UniParc]FASTAAdd to Basket

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    MSETLENLLQ ETRTFPPPPE FASRANGTAE LYEKADADHE GFWAEQARTY    50
    VSWSKDFTQT LQWDTPFAKW FPDGELNACY NAVDRHVEAG NGDRVAIHWI 100
    GEPEGDTREI TYSDLHSSVQ RAANALQDIG VRKGDTVAIY LPMIPEAAVA 150
    MLACARIGAP HSVVFGGFSA EALHSRIDDA GAKVVITSDG GYRRGSASPL 200
    KPAVDAALDH GDTSVEKVLV VKRTGQETPW TEGRDLWWDE VLEQAAPTHE 250
    APAHESEHPL FILYTSGTTG KPKGIFHTTG GYLTQCAYTN AVVHDVHPES 300
    DVYWCTADIG WVTGHSYIVY GPLTLGATQV MYEGTPDTPH QGRFWEIVEQ 350
    KKVTILYTAP TAIRTFMKWG DDIPGKFDLS SLRLLGSVGE PINPEAWMWY 400
    RRVIGGDRCP IVDTWWQTET GAIMVSPLPG VTATKPGSAQ RAIPGIGAEV 450
    VDDMAQPVPN GHGGYLVLTK PWPAMLRGIW GDPERYKETY WSRFEGLYFA 500
    GDGAKKDDDG DIWLLGRVDD VMNVSGHRLS TTEIESALVS HPKVAEAAVV 550
    GATDETTGQA VVAFVILRSE AVAEADEPGE GADIVAELRN HVAKEIGPIA 600
    KPRSIMIVPE LPKTRSGKIM RRLLRDVAEN RQVGDVTTLA DSSVMELIKT 650
    GLNKGDGD 658
    Length:658
    Mass (Da):71,897
    Last modified:March 8, 2011 - v1
    Checksum:i678660EA24A7E85D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002343 Genomic DNA. Translation: ADU47061.1.
    RefSeqiYP_004097788.1. NC_014830.1.

    Genome annotation databases

    EnsemblBacteriaiADU47061; ADU47061; Intca_0516.
    GeneIDi10083310.
    KEGGiica:Intca_0516.
    PATRICi45242428. VBIIntCal153415_0517.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002343 Genomic DNA. Translation: ADU47061.1 .
    RefSeqi YP_004097788.1. NC_014830.1.

    3D structure databases

    ProteinModelPortali E6S985.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADU47061 ; ADU47061 ; Intca_0516 .
    GeneIDi 10083310.
    KEGGi ica:Intca_0516.
    PATRICi 45242428. VBIIntCal153415_0517.

    Phylogenomic databases

    HOGENOMi HOG000229981.
    KOi K01895.

    Enzyme and pathway databases

    BioCyci ICAL710696:GH9U-529-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIPImported.

    Entry informationi

    Entry nameiE6S985_INTC7
    AccessioniPrimary (citable) accession number: E6S985
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 8, 2011
    Last sequence update: March 8, 2011
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3