Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E6RWN3 (E6RWN3_CAMJS) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL ADT72581.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:CJS3_0846 EMBL ADT72581.1
OrganismCampylobacter jejuni subsp. jejuni (strain S3) [Complete proteome] [HAMAP] EMBL ADT72581.1
Taxonomic identifier718271 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain133 – 327195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding159 – 21153ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2841Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2961Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2961Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2981Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E6RWN3 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: CDFAF5AB2726749A

FASTA34639,800
        10         20         30         40         50         60 
MKFAILFGGN SYEHEISIVS AVVLKKVINQ NLEFIFCDEE RRFYHIPSEK MNSKTFSTKA 

        70         80         90        100        110        120 
YKKEKELFIK QGGFFSKGFL KENKLECECV INLIHGRDGE DGKIAALFEF YSIKFIGPRL 

       130        140        150        160        170        180 
EASVLSFNKE LTKLYAKSVG VKTLDYTMVR KGQNSKEKLS FPCIIKPARL GSSIGISIVK 

       190        200        210        220        230        240 
DEKDLEYAKD VGFEFDNDLV VEEFKNNIKE YNLAGCMIND EFVFSIIEEP KKKEFLDFEQ 

       250        260        270        280        290        300 
KYLSFSGHNE LIEANLSEEL KEKLKDSFKK IYNPLFKGAL IRCDFFILDN EIYLNEINPN 

       310        320        330        340 
PGSLANYLFK DFSTTLNALA DQISLEKMIK ISYNFLHSIN GQKGKL 

« Hide

References

[1]"Complete genome sequence of Campylobacter jejuni strain S3."
Cooper K.K., Cooper M.A., Zuccolo A., Law B., Joens L.A.
J. Bacteriol. 193:1491-1492(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S3 EMBL ADT72581.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001960 Genomic DNA. Translation: ADT72581.1.
RefSeqYP_005659417.1. NC_017281.1.

3D structure databases

ProteinModelPortalE6RWN3.
SMRE6RWN3. Positions 1-323.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADT72581; ADT72581; CJS3_0846.
GeneID12245965.
KEGGcjs:CJS3_0846.
PATRIC45418562. VBICamJej157961_0843.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycCJEJ718271:GLAE-848-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6RWN3_CAMJS
AccessionPrimary (citable) accession number: E6RWN3
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: February 19, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)