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E6RGE2

- E6RGE2_PSEU9

UniProt

E6RGE2 - E6RGE2_PSEU9

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Pseudoalteromonas sp. (strain SM9913)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (08 Mar 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi454 – 4541MagnesiumUniRule annotation
    Metal bindingi460 – 4601Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi463 – 4631MagnesiumUniRule annotation
    Metal bindingi464 – 4641MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciPSP234831:GH93-1166-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Gene namesi
    Name:pheTUniRule annotationImported
    Ordered Locus Names:PSM_A1142Imported
    OrganismiPseudoalteromonas sp. (strain SM9913)Imported
    Taxonomic identifieri234831 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
    ProteomesiUP000007933: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 148110tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini401 – 47676B5UniRule annotationAdd
    BLAST
    Domaini701 – 79494FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation
    Contains B5 domain.SAAS annotation
    Contains Bdomain.SAAS annotation
    Contains FDX-ACB domain.SAAS annotation
    Contains tRNA-binding domain.SAAS annotation

    Phylogenomic databases

    HOGENOMiHOG000292085.
    KOiK01890.
    OMAiAISSTFC.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E6RGE2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFSEKWLRE WVNPAIDTQA LSEQLSMAGL EVDGVEPAAA KFNGVVVGEV    50
    VECGQHPDAD KLRVTKINVG GDELLDIVCG APNCRQGIKV AVATVGAVLP 100
    GDFKIKKAKL RGQPSHGMLC AFDELGISEE GDGIMELPLD APIGADLREY 150
    LALDDNIIDV DLTPNRGDCL GIKGLAREVG VLNSIDVNTL SIPAVTPTIE 200
    DKVSIELVND DACPRYLGRV IKGINLDAET PLWMVEKLRR SGVRSIDPVV 250
    DVTNFVLLEL GHPMHAFDLN AIEGGIKVRS ATPGEELVLL DGNTAKLNES 300
    TLVIADHHKA LAIAGIFGGE QSGVTEKTAD ILLESAFFNP VAIAGQARSY 350
    GLHTDASHRY ERGVDFALQH DAMERATALL LEIVGGNAGP IVEAVAADKL 400
    PKVTEVRLRR SRLDRVIGHH IDDAKVTDIL TRLGLDVKFD NDSWSADVPS 450
    YRFDIRIEED LIEEVARVYG YNSIPNVAPT AKLKMTTHDE STVALSRFRN 500
    ALVTRGYQEA ITYSFVDPKA QAILHPECNA LVLPHPISIE MSAMRVSLMP 550
    GLLASVAYNQ NRQQPRIRLF EHGLKFISDE SAENGVYQVP VIGGVITGLV 600
    HGEHWVEEKR NVDFYDLKGD VEALLAITND VSRFEIKAEQ SDGLHPGQSA 650
    VIYVDGKKVG FFGALHPQAQ KSLDINNATF VFEIEMSALE KRNLPLAVGV 700
    SKFPSNRRDI AILVQDSVKI GDILNVIGKV GGNQLVDLNL FDVYKGKGIE 750
    PNYKSLAIAL TLQAVDRTLE EKDINQVVDN VVAALAEQFN ASLRD 795
    Length:795
    Mass (Da):86,535
    Last modified:March 8, 2011 - v1
    Checksum:iE69207F9EF20DBB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001796 Genomic DNA. Translation: ADT68084.1.
    RefSeqiYP_004068235.1. NC_014803.1.

    Genome annotation databases

    EnsemblBacteriaiADT68084; ADT68084; PSM_A1142.
    GeneIDi10038028.
    KEGGipsm:PSM_A1142.
    PATRICi45329714. VBIPseSp136886_1752.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001796 Genomic DNA. Translation: ADT68084.1 .
    RefSeqi YP_004068235.1. NC_014803.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADT68084 ; ADT68084 ; PSM_A1142 .
    GeneIDi 10038028.
    KEGGi psm:PSM_A1142.
    PATRICi 45329714. VBIPseSp136886_1752.

    Phylogenomic databases

    HOGENOMi HOG000292085.
    KOi K01890.
    OMAi AISSTFC.

    Enzyme and pathway databases

    BioCyci PSP234831:GH93-1166-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomics reveals a deep-sea sediment-adapted life style of Pseudoalteromonas sp. SM9913."
      Qin Q.L., Li Y., Zhang Y.J., Zhou Z.M., Zhang W.X., Chen X.L., Zhang X.Y., Zhou B.C., Wang L., Zhang Y.Z.
      ISME J. 5:274-284(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SM9913Imported.

    Entry informationi

    Entry nameiE6RGE2_PSEU9
    AccessioniPrimary (citable) accession number: E6RGE2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 8, 2011
    Last sequence update: March 8, 2011
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3