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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Pseudoalteromonas sp. (strain SM9913)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi454 – 4541MagnesiumUniRule annotation
Metal bindingi460 – 4601Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi463 – 4631MagnesiumUniRule annotation
Metal bindingi464 – 4641MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPSP234831:GH93-1166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotationSAAS annotation (EC:6.1.1.20UniRule annotationSAAS annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Gene namesi
Name:pheTUniRule annotationImported
Ordered Locus Names:PSM_A1142Imported
OrganismiPseudoalteromonas sp. (strain SM9913)Imported
Taxonomic identifieri234831 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000007933: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 148110tRNA-bindingUniRule annotationAdd
BLAST
Domaini401 – 47676B5UniRule annotationAdd
BLAST
Domaini701 – 79494FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000292085.
KOiK01890.
OMAiRQYGLHT.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

E6RGE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSEKWLRE WVNPAIDTQA LSEQLSMAGL EVDGVEPAAA KFNGVVVGEV
60 70 80 90 100
VECGQHPDAD KLRVTKINVG GDELLDIVCG APNCRQGIKV AVATVGAVLP
110 120 130 140 150
GDFKIKKAKL RGQPSHGMLC AFDELGISEE GDGIMELPLD APIGADLREY
160 170 180 190 200
LALDDNIIDV DLTPNRGDCL GIKGLAREVG VLNSIDVNTL SIPAVTPTIE
210 220 230 240 250
DKVSIELVND DACPRYLGRV IKGINLDAET PLWMVEKLRR SGVRSIDPVV
260 270 280 290 300
DVTNFVLLEL GHPMHAFDLN AIEGGIKVRS ATPGEELVLL DGNTAKLNES
310 320 330 340 350
TLVIADHHKA LAIAGIFGGE QSGVTEKTAD ILLESAFFNP VAIAGQARSY
360 370 380 390 400
GLHTDASHRY ERGVDFALQH DAMERATALL LEIVGGNAGP IVEAVAADKL
410 420 430 440 450
PKVTEVRLRR SRLDRVIGHH IDDAKVTDIL TRLGLDVKFD NDSWSADVPS
460 470 480 490 500
YRFDIRIEED LIEEVARVYG YNSIPNVAPT AKLKMTTHDE STVALSRFRN
510 520 530 540 550
ALVTRGYQEA ITYSFVDPKA QAILHPECNA LVLPHPISIE MSAMRVSLMP
560 570 580 590 600
GLLASVAYNQ NRQQPRIRLF EHGLKFISDE SAENGVYQVP VIGGVITGLV
610 620 630 640 650
HGEHWVEEKR NVDFYDLKGD VEALLAITND VSRFEIKAEQ SDGLHPGQSA
660 670 680 690 700
VIYVDGKKVG FFGALHPQAQ KSLDINNATF VFEIEMSALE KRNLPLAVGV
710 720 730 740 750
SKFPSNRRDI AILVQDSVKI GDILNVIGKV GGNQLVDLNL FDVYKGKGIE
760 770 780 790
PNYKSLAIAL TLQAVDRTLE EKDINQVVDN VVAALAEQFN ASLRD
Length:795
Mass (Da):86,535
Last modified:March 8, 2011 - v1
Checksum:iE69207F9EF20DBB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001796 Genomic DNA. Translation: ADT68084.1.
RefSeqiYP_004068235.1. NC_014803.1.

Genome annotation databases

EnsemblBacteriaiADT68084; ADT68084; PSM_A1142.
GeneIDi10038028.
KEGGipsm:PSM_A1142.
PATRICi45329714. VBIPseSp136886_1752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001796 Genomic DNA. Translation: ADT68084.1.
RefSeqiYP_004068235.1. NC_014803.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADT68084; ADT68084; PSM_A1142.
GeneIDi10038028.
KEGGipsm:PSM_A1142.
PATRICi45329714. VBIPseSp136886_1752.

Phylogenomic databases

HOGENOMiHOG000292085.
KOiK01890.
OMAiRQYGLHT.

Enzyme and pathway databases

BioCyciPSP234831:GH93-1166-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomics reveals a deep-sea sediment-adapted life style of Pseudoalteromonas sp. SM9913."
    Qin Q.L., Li Y., Zhang Y.J., Zhou Z.M., Zhang W.X., Chen X.L., Zhang X.Y., Zhou B.C., Wang L., Zhang Y.Z.
    ISME J. 5:274-284(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SM9913Imported.

Entry informationi

Entry nameiE6RGE2_PSEU9
AccessioniPrimary (citable) accession number: E6RGE2
Entry historyi
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: March 4, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.