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E6NPL0 (E6NPL0_HELPL) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL BAJ58286.1
Ordered Locus Names:HPF32_0704 EMBL BAJ58286.1
OrganismHelicobacter pylori (strain F32) [Complete proteome] [HAMAP] EMBL BAJ58286.1
Taxonomic identifier102608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 332199ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding161 – 21656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2881Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3001Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E6NPL0 [UniParc].

Last modified March 8, 2011. Version 1.
Checksum: B88AC6648CCB5947

FASTA34739,616
        10         20         30         40         50         60 
MEFCVLFGGA SFEHEISIVS AIALKETLKD RIKYFIFLDE NHHFYLVEES NMHSKYFAQI 

        70         80         90        100        110        120 
KEKKLPPLIL THNGLLKNSF LGTKIIELPL VINLVHGGDG EDGKLASLLE FYRIAFIGPR 

       130        140        150        160        170        180 
IEASVLSYSK YLTKLYAKDL GIKTLDCVLV NEKNRANALD LIGFNLPFII KPSNAGSSLG 

       190        200        210        220        230        240 
VSVVKEEKEL IYALDGAFEY SKEVLIEPFI QGVKEYNLAG CKIKKDFCFS YVEEPNKQEF 

       250        260        270        280        290        300 
LDFKQKYLDF SRNKAPKANL SNTLEEQLKE NFKKLYNDLF DGALIRCDFF VIENEVYLNE 

       310        320        330        340 
INPIPGSLAN YLFDDFKTTL ENLAQSLPKT PKIPIKNSYL LQIQKNK 

« Hide

References

[1]"Evolution in an oncogenic bacterial species with extreme genome plasticity: Helicobacter pylori East Asian genomes."
Kawai M., Furuta Y., Yahara K., Tsuru T., Oshima K., Handa N., Takahashi N., Yoshida M., Azuma T., Hattori M., Uchiyama I., Kobayashi I.
BMC Microbiol. 11:104-104(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F32 EMBL BAJ58286.1.
[2]"Birth and death of genes linked to chromosomal inversion."
Furuta Y., Kawai M., Yahara K., Takahashi N., Handa N., Tsuru T., Oshima K., Yoshida M., Azuma T., Hattori M., Uchiyama I., Kobayashi I.
Proc. Natl. Acad. Sci. U.S.A. 108:1501-1506(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F32 EMBL BAJ58286.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP011943 Genomic DNA. Translation: BAJ58286.1.
RefSeqYP_005775928.1. NC_017366.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAJ58286; BAJ58286; HPF32_0704.
GeneID12355527.
KEGGheq:HPF32_0704.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycHPYL102608:GLEE-723-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE6NPL0_HELPL
AccessionPrimary (citable) accession number: E6NPL0
Entry history
Integrated into UniProtKB/TrEMBL: March 8, 2011
Last sequence update: March 8, 2011
Last modified: July 9, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)