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Protein

Pheromone-processing carboxypeptidase KEX1

Gene

KEX1

Organism
Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death (By similarity).By similarity

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei182 – 1821PROSITE-ProRule annotation
Active sitei382 – 3821PROSITE-ProRule annotation
Active sitei444 – 4441PROSITE-ProRule annotation

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Apoptosis

Protein family/group databases

MEROPSiS10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)
Alternative name(s):
Carboxypeptidase D
Gene namesi
Name:KEX1
ORF Names:Lema_P047160
OrganismiLeptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam)
Taxonomic identifieri985895 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaeLeptosphaeriaceaeLeptosphaeriaLeptosphaeria maculans complex
ProteomesiUP000002668: Whole genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 515480LumenalSequence AnalysisAdd
BLAST
Transmembranei516 – 53621HelicalSequence AnalysisAdd
BLAST
Topological domaini537 – 641105CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Chaini36 – 641606Pheromone-processing carboxypeptidase KEX1PRO_0000411923Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi522 – 5276Poly-Val

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiE5R540.
OMAiYIARAIV.
OrthoDBiEOG7TJ3SJ.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E5R540-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASTYSTPRW RTALLGGFLT TLPWLSSGMA GKTQADYFIK SLPGAPEPLL
60 70 80 90 100
KMHAGHIEVD AEHNGNLFFW HYENRHIADR QRTVLWLNGG PGCSSMDGAL
110 120 130 140 150
MEVGPYRVQA DGNLHYNNGS WDEFANLLFV DQPVGTGFSY VNTDSYLTEL
160 170 180 190 200
DQMANHMVIF LEKWFGLFPE YEHDDLYIAG ESYAGQHIPY IARAIVKRNK
210 220 230 240 250
EQGKTPWALK GLLIGNGWIS PVDQYLSYIP YAYQNGLMKA DSDMAKRVEN
260 270 280 290 300
QQRICIKKLE DGGMDAVDTN DCEQIMVNIL EETKDRKADR MNQCVNMYDI
310 320 330 340 350
RLRDDASCGM NWPPDLASVT PYLRRPDVIQ ALHINPDKKT GWQECNGAVS
360 370 380 390 400
GHFRAKKSEP SVRFLPELIP EVPTLLFSGD KDFICNHIGT EEMIKNMQWS
410 420 430 440 450
GGKGFEVTPG VWAPKQDWTF EGEAAGSWQE ARNLTYVVFY NSSHMVPFDY
460 470 480 490 500
PRRTRDMLDR FMGVDIEQIG GVPSDSRIDG EKGPLTSVGD HPNSTRAEED
510 520 530 540 550
KATDVKQAEW KAYYRSGQVA LVVVVIVAAL WGIFLWRSRR RHTKTAYQGD
560 570 580 590 600
DGDEGRESLL TGMGLDNFRR KERRSDLEAA DFDERELDDL DGASKKPGNG
610 620 630 640
YASLGSEKER QSHNDSTFSL GGDSDDEAGS SDGPKRKGGS S
Length:641
Mass (Da):72,004
Last modified:February 8, 2011 - v1
Checksum:iEBC87398725E1F59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP929083 Genomic DNA. Translation: CBX92010.1.
RefSeqiXP_003835375.1. XM_003835327.1.

Genome annotation databases

EnsemblFungiiCBX92010; CBX92010; LEMA_P047160.1.
GeneIDi13284615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP929083 Genomic DNA. Translation: CBX92010.1.
RefSeqiXP_003835375.1. XM_003835327.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS10.007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCBX92010; CBX92010; LEMA_P047160.1.
GeneIDi13284615.

Phylogenomic databases

InParanoidiE5R540.
OMAiYIARAIV.
OrthoDBiEOG7TJ3SJ.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8.

Entry informationi

Entry nameiKEX1_LEPMJ
AccessioniPrimary (citable) accession number: E5R540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: February 8, 2011
Last modified: January 7, 2015
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.