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Protein

Methionine aminopeptidase 2-2

Gene

MGYG_01859

Organism
Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei199SubstrateUniRule annotation1
Metal bindingi219Divalent metal cation 1UniRule annotation1
Metal bindingi230Divalent metal cation 1UniRule annotation1
Metal bindingi230Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi299Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei307SubstrateUniRule annotation1
Metal bindingi335Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi430Divalent metal cation 1UniRule annotation1
Metal bindingi430Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2-2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2-2UniRule annotation
Short name:
MetAP 2-2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
ORF Names:MGYG_01859
OrganismiArthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum)
Taxonomic identifieri535722 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesArthrodermataceaeNannizzia
Proteomesi
  • UP000002669 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004075961 – 449Methionine aminopeptidase 2-2Add BLAST449

Interactioni

Protein-protein interaction databases

STRINGi535722.XP_003177796.1.

Structurei

3D structure databases

ProteinModelPortaliE5R3Z8.
SMRiE5R3Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 76Lys-richAdd BLAST16

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2775. Eukaryota.
COG0024. LUCA.
InParanoidiE5R3Z8.
OrthoDBiEOG092C3NQP.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiView protein in InterPro
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00557. Peptidase_M24. 1 hit.
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiView protein in PROSITE
PS01202. MAP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

E5R3Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQAAPELA KLDLNKNTGS AEASTVPASG SDKDDAENEG DSDDDRDDEQ
60 70 80 90 100
TGGSAEVNAE KKKKKKRPKK KKKTAKVQSV PPRIPLTTLF PNNSFPEGEI
110 120 130 140 150
VEYLNENSYR TTNEEKRHLD RMNNDFLAEY RQAAEIHRQV RQYAQKELIK
160 170 180 190 200
PGATLTDIAE GIEDGVRHLT GHLGLEEGDS LVAGMGFPTG LNINHCAAHY
210 220 230 240 250
SPNAGNKVVL QHGDVMKVDF GVHVNGRIVD SAFTVAFDPV FDPLLTAVKE
260 270 280 290 300
ATNTGIKEAG IDVRMSDIGA AIQETMESYE LELNGTSYPI KAIRNLNGHT
310 320 330 340 350
IGQYEIHGGV NGKSVPIVKG GDQTKMEEGE TYAIETFGST GKGYVRDDME
360 370 380 390 400
TSHYAKVPSA PSVPLRLTSA KNLYSLINKN FGTLPFCRRY LDRLGQEKYL
410 420 430 440
LGLNNLVSSG LVDAYPPLCD VKGSYTAQFE HTILLRPNVK EVISRGDDY
Length:449
Mass (Da):49,131
Last modified:February 8, 2011 - v1
Checksum:iA846835A274F41C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS989822 Genomic DNA. Translation: EFQ98844.1.
RefSeqiXP_003177796.1. XM_003177748.1.

Genome annotation databases

EnsemblFungiiEFQ98844; EFQ98844; MGYG_01859.
GeneIDi10033131.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMAP22_ARTGP
AccessioniPrimary (citable) accession number: E5R3Z8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: February 8, 2011
Last modified: May 10, 2017
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families