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Protein

Endonuclease III-like protein 1

Gene

NTHL1

Organism
Homo sapiens (Human)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei220Nucleophile; for N-glycosylase activityUniRule annotation1
Sitei239Important for catalytic activityUniRule annotation1
Metal bindingi290Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi297Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi300Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi306Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III-like protein 1UniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
Alternative name(s):
Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Short name:
DNA glycosylase/AP lyaseUniRule annotation
Gene namesi
Name:NTHL1UniRule annotationImported
ORF Names:hCG_1989994Imported
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Organism-specific databases

EuPathDBiHostDB:ENSG00000065057.7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation, NucleusUniRule annotation

Expressioni

Gene expression databases

BgeeiENSG00000065057.

Interactioni

Subunit structurei

Interacts with YBX1.UniRule annotation

Structurei

3D structure databases

SMRiE5KTI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 288ENDO3cInterPro annotationAdd BLAST151

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1921. Eukaryota.
COG0177. LUCA.
KOiK10773.
OMAiWRNKVKY.
OrthoDBiEOG091G0IVB.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
HAMAPiMF_03183. Endonuclease_III_Nth. 1 hit.
InterProiView protein in InterPro
IPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endonuclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR023170. HTH_base_excis_C.
IPR030841. NTH1.
PfamiView protein in Pfam
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
SMARTiView protein in SMART
SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiView protein in PROSITE
PS01155. ENDONUCLEASE_III_2. 1 hit.

Sequencei

Sequence statusi: Complete.

E5KTI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK
60 70 80 90 100
SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR
110 120 130 140 150
AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA
160 170 180 190 200
MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY
210 220 230 240 250
GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR
260 270 280 290 300
WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC
310
LNQALCPAAQ GL
Length:312
Mass (Da):34,390
Last modified:February 8, 2011 - v1
Checksum:i379816A1E0B45050
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ206266 Genomic DNA. Translation: ADP92214.1.
HQ206267 Genomic DNA. Translation: ADP92215.1.
HQ206268 Genomic DNA. Translation: ADP92216.1.
HQ206269 Genomic DNA. Translation: ADP92217.1.
HQ206270 Genomic DNA. Translation: ADP92218.1.
HQ206271 Genomic DNA. Translation: ADP92219.1.
HQ206272 Genomic DNA. Translation: ADP92220.1.
HQ206273 Genomic DNA. Translation: ADP92221.1.
HQ206274 Genomic DNA. Translation: ADP92222.1.
HQ206275 Genomic DNA. Translation: ADP92223.1.
HQ206276 Genomic DNA. Translation: ADP92224.1.
HQ206277 Genomic DNA. Translation: ADP92225.1.
HQ206278 Genomic DNA. Translation: ADP92226.1.
HQ206279 Genomic DNA. Translation: ADP92227.1.
HQ206280 Genomic DNA. Translation: ADP92228.1.
HQ206281 Genomic DNA. Translation: ADP92229.1.
HQ206282 Genomic DNA. Translation: ADP92230.1.
HQ206283 Genomic DNA. Translation: ADP92231.1.
HQ206284 Genomic DNA. Translation: ADP92232.1.
HQ206285 Genomic DNA. Translation: ADP92233.1.
HQ206286 Genomic DNA. Translation: ADP92234.1.
HQ206287 Genomic DNA. Translation: ADP92235.1.
HQ206288 Genomic DNA. Translation: ADP92236.1.
HQ206289 Genomic DNA. Translation: ADP92237.1.
HQ206290 Genomic DNA. Translation: ADP92238.1.
HQ206291 Genomic DNA. Translation: ADP92239.1.
HQ206292 Genomic DNA. Translation: ADP92240.1.
HQ206293 Genomic DNA. Translation: ADP92241.1.
HQ206294 Genomic DNA. Translation: ADP92242.1.
HQ206295 Genomic DNA. Translation: ADP92243.1.
HQ206296 Genomic DNA. Translation: ADP92244.1.
HQ206297 Genomic DNA. Translation: ADP92245.1.
HQ206298 Genomic DNA. Translation: ADP92246.1.
HQ206299 Genomic DNA. Translation: ADP92247.1.
HQ206300 Genomic DNA. Translation: ADP92248.1.
HQ206301 Genomic DNA. Translation: ADP92249.1.
HQ206302 Genomic DNA. Translation: ADP92250.1.
HQ206303 Genomic DNA. Translation: ADP92251.1.
HQ206304 Genomic DNA. Translation: ADP92252.1.
HQ206305 Genomic DNA. Translation: ADP92253.1.
CH471112 Genomic DNA. Translation: EAW85562.1.
RefSeqiNP_002519.1. NM_002528.6.
UniGeneiHs.66196.

Genome annotation databases

GeneIDi4913.
KEGGihsa:4913.

Similar proteinsi

Entry informationi

Entry nameiE5KTI5_HUMAN
AccessioniPrimary (citable) accession number: E5KTI5
Entry historyiIntegrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: November 22, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.