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Protein

Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial

Gene

SUCLA2

Organism
Homo sapiens (Human)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (SUCLG1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98ATPUniRule annotation1
Binding sitei166ATPUniRule annotation1
Metal bindingi258MagnesiumUniRule annotation1
Metal bindingi272MagnesiumUniRule annotation1
Binding sitei323Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 107ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processTricarboxylic acid cycleUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase beta chainUniRule annotation
Short name:
SCS-betaUniRule annotation
Gene namesi
Name:SUCLA2Imported
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

MitochondrionUniRule annotation

Expressioni

Gene expression databases

BgeeiENSG00000136143.
ExpressionAtlasiE5KS60. baseline and differential.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.UniRule annotation

Protein-protein interaction databases

IntActiE5KS60. 4 interactors.
STRINGi9606.ENSP00000367923.

Structurei

3D structure databases

SMRiE5KS60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 288ATP-graspInterPro annotationAdd BLAST228

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 382Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
KOiK01900.
OMAiYIESGCD.
OrthoDBiEOG091G07T9.
PhylomeDBiE5KS60.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E5KS60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASMFYGRL VAVATLRNHR PRTAQRAAAQ VLGSSGLFNN HGLQVQQQQQ
60 70 80 90 100
RNLSLHEYMS MELLQEAGVS VPKGYVAKSP DEAYAIAKKL GSKDVVIKAQ
110 120 130 140 150
VLAGGRGKGT FESGLKGGVK IVFSPEEAKA VSSQMIGKKL FTKQTGEKGR
160 170 180 190 200
ICNQVLVCER KYPRREYYFA ITMERSFQGP VLIGSSHGGV NIEDVAAESP
210 220 230 240 250
EAIIKEPIDI EEGIKKEQAL QLAQKMGFPP NIVESAAENM VKLYSLFLKY
260 270 280 290 300
DATMIEINPM VEDSDGAVLC MDAKINFDSN SAYRQKKIFD LQDWTQEDER
310 320 330 340 350
DKDAAKANLN YIGLDGNIGC LVNGAGLAMA TMDIIKLHGG TPANFLDVGG
360 370 380 390 400
GATVHQVTEA FKLITSDKKV LAILVNIFGG IMRCDVIAQG IVMAVKDLEI
410 420 430 440 450
KIPVVVRLQG TRVDDAKALI ADSGLKILAC DDLDEAARMV VKLSEIVTLA
460
KQAHVDVKFQ LPI
Length:463
Mass (Da):50,317
Last modified:February 8, 2011 - v1
Checksum:i1E1651728AF3B5CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei463Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ205871 Genomic DNA. Translation: ADP91739.1.
HQ205872 Genomic DNA. Translation: ADP91740.1.
HQ205873 Genomic DNA. Translation: ADP91741.1.
HQ205874 Genomic DNA. Translation: ADP91742.1.
HQ205876 Genomic DNA. Translation: ADP91744.1.
HQ205877 Genomic DNA. Translation: ADP91745.1.
HQ205879 Genomic DNA. Translation: ADP91747.1.
HQ205882 Genomic DNA. Translation: ADP91750.1.
HQ205883 Genomic DNA. Translation: ADP91751.1.
HQ205886 Genomic DNA. Translation: ADP91754.1.
HQ205891 Genomic DNA. Translation: ADP91759.1.
HQ205894 Genomic DNA. Translation: ADP91762.1.
HQ205897 Genomic DNA. Translation: ADP91765.1.
KU178442 mRNA. Translation: ALQ33900.1.
RefSeqiNP_003841.1. NM_003850.2.
UniGeneiHs.743361.

Genome annotation databases

GeneIDi8803.
KEGGihsa:8803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ205871 Genomic DNA. Translation: ADP91739.1.
HQ205872 Genomic DNA. Translation: ADP91740.1.
HQ205873 Genomic DNA. Translation: ADP91741.1.
HQ205874 Genomic DNA. Translation: ADP91742.1.
HQ205876 Genomic DNA. Translation: ADP91744.1.
HQ205877 Genomic DNA. Translation: ADP91745.1.
HQ205879 Genomic DNA. Translation: ADP91747.1.
HQ205882 Genomic DNA. Translation: ADP91750.1.
HQ205883 Genomic DNA. Translation: ADP91751.1.
HQ205886 Genomic DNA. Translation: ADP91754.1.
HQ205891 Genomic DNA. Translation: ADP91759.1.
HQ205894 Genomic DNA. Translation: ADP91762.1.
HQ205897 Genomic DNA. Translation: ADP91765.1.
KU178442 mRNA. Translation: ALQ33900.1.
RefSeqiNP_003841.1. NM_003850.2.
UniGeneiHs.743361.

3D structure databases

SMRiE5KS60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiE5KS60. 4 interactors.
STRINGi9606.ENSP00000367923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8803.
KEGGihsa:8803.

Organism-specific databases

CTDi8803.

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
KOiK01900.
OMAiYIESGCD.
OrthoDBiEOG091G07T9.
PhylomeDBiE5KS60.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999.

Miscellaneous databases

GenomeRNAii8803.

Gene expression databases

BgeeiENSG00000136143.
ExpressionAtlasiE5KS60. baseline and differential.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE5KS60_HUMAN
AccessioniPrimary (citable) accession number: E5KS60
Entry historyiIntegrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: January 18, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.