E5KIY2 (E5KIY2_ECOLX) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Beta-lactamase NDM-1 EMBL AEH43774.1

Submitted name:
Metallo beta lactamase NDM-1 EMBL AGB57374.1
Submitted name:
Metallo-beta-lactamase EMBL ADP20459.1
Submitted name:
NDM-1 EMBL AFI72857.1
Submitted name:
NDM-1 metallo-beta-lactamase EMBL AEN71485.1
Submitted name:
New Delhi metallo-beta-lactamase EMBL ADY00041.1
Submitted name:
New Delhi metallo-beta-lactamase-1 EMBL AFN73221.1

Gene names

Name:	bla NDM-1 EMBL AFN73221.1
Synonyms:	blaNDM-1 EMBL ADY00041.1
ORF Names:	NDM1Dok01_N0175 EMBL BAK69668.1, pNDM102337_147 EMBL AEP32605.1, pNDM10505_149 EMBL AEN71485.1

Encoded on

Plasmid pABC85 Ref.7 Ref.12 EMBL AGB57374.1
Plasmid pNDM-1_Dok01 Ref.6 Ref.7 EMBL BAK69668.1
Plasmid unnamed Ref.7 Ref.10 EMBL AFN73221.1
Plasmid pGUE-NDM Ref.7 Ref.9 EMBL AFI72857.1
Plasmid pBJ01 Ref.7 Ref.11 EMBL AFU75168.1
Plasmid p271A Ref.3 Ref.7 EMBL AEK64842.1
Plasmid pNDM-HK Ref.5 Ref.7 EMBL ADY00041.1
Plasmid pNDM10505 Ref.7 Ref.8 EMBL AEN71485.1
Plasmid pNDM102337 Ref.7 Ref.13 EMBL AEP32605.1

Organism

Escherichia coli EMBL ADP20459.1

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Metal-binding PDB 3S0Z Zinc PDB 3S0Z
Technical term	3D-structure PDB 3S0Z Plasmid EMBL ADY00041.1
Gene Ontology (GO)
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	120	1	Zinc; via tele nitrogen PDB 3S0Z
	Metal binding	122	1	Zinc PDB 3S0Z
	Metal binding	189	1	Zinc; via tele nitrogen PDB 3S0Z
	Metal binding	208	1	Zinc PDB 3S0Z

Sequences

	Sequence		Length	Mass (Da)
	E5KIY2 [UniParc]. Last modified February 8, 2011. Version 1. Checksum: 1B6009120B945F14	FASTA	270	28,499
	10 20 30 40 50 60 MELPNIMHPV AKLSTALAAA LMLSGCMPGE IRPTIGQQME TGDQRFGDLV FRQLAPNVWQ 70 80 90 100 110 120 HTSYLDMPGF GAVASNGLIV RDGGRVLVVD TAWTDDQTAQ ILNWIKQEIN LPVALAVVTH 130 140 150 160 170 180 AHQDKMGGMD ALHAAGIATY ANALSNQLAP QEGMVAAQHS LTFAANGWVE PATAPNFGPL 190 200 210 220 230 240 KVFYPGPGHT SDNITVGIDG TDIAFGGCLI KDSKAKSLGN LGDADTEHYA ASARAFGAAF 250 260 270 PKASMIVMSH SAPDSRAAIT HTARMADKLR

References

[1]	"Emergence of metallo-?-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia." Poirel L., Lagrutta E., Taylor P., Pham J., Nordmann P. Antimicrob. Agents Chemother. 54:4914-4916(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 271 EMBL ADP20459.1.
[2]	"The entire NDM-1 gene sequence of NDM-1-positive E. coli isolated in Japan." Yamamoto T. Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: NDM-1 Dok01 EMBL BAK40112.1.
[3]	"Analysis of the Resistome of a Multidrug-Resistant NDM-1-Producing Escherichia coli Strain by High-Throughput Genome Sequencing." Poirel L., Bonnin R.A., Nordmann P. Antimicrob. Agents Chemother. 55:4224-4229(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 271 EMBL AEK64842.1.
[4]	"First Description of an Escherichia coli Strain Producing NDM-1 Carbapenemase in Spain." Sole M., Pitart C., Roca I., Fabrega A., Salvador P., Munoz L., Oliveira I., Gascon J., Marco F., Vila J. Antimicrob. Agents Chemother. 55:4402-4404(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: DVR22 EMBL AEH43774.1.
[5]	"Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong." Ho P.L., Lo W.U., Yeung M.K., Lin C.H., Chow K.H., Ang I., Tong A.H., Bao J.Y., Lok S., Lo J.Y. PLoS ONE 6:E17989-E17989(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: HK-01 EMBL ADY00041.1.
[6]	"Complete Sequencing of the blaNDM-1-Positive IncA/C Plasmid from Escherichia coli ST38 Isolate Suggests a Possible Origin from Plant Pathogens." Sekizuka T., Matsui M., Yamane K., Takeuchi F., Ohnishi M., Hishinuma A., Arakawa Y., Kuroda M. PLoS ONE 6:e25334-e25334(2011) Cited for: NUCLEOTIDE SEQUENCE. Strain: NDM-1 Dok01 EMBL BAK69668.1.
[7]	"A structural view of the antibiotic degradation enzyme NDM-1 from a superbug." Guo Y., Wang J., Niu G., Shui W., Sun Y., Zhou H., Zhang Y., Yang C., Lou Z., Rao Z. Protein Cell 2:384-394(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 47-270 IN COMPLEX WITH ZINC.
[8]	"Inc A/C plasmids harbouring the NDM-1 metallo-beta-lactamase." Boyd D.A., Mataseje L.F., Mulvey M.R. Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: N10-0505 EMBL AEN71485.1.
[9]	"Characterization of an IncFII Plasmid Encoding NDM-1 from Escherichia coli ST131." Bonnin R.A., Poirel L., Carattoli A., Nordmann P. PLoS ONE 7:E34752-E34752(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: GUE EMBL AFI72857.1.
[10]	"First New Delhi Metallo-beta-Lactamase 1-Producing Escherichia coli isolated in China." Liu Z. Submitted (MAY-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BJ01 EMBL AFN73221.1.
[11]	Comotor D., Becsagh P., Rakhely G., Schneider G., Kovacs T. Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BJ01 EMBL AFU75168.1.
[12]	"Emergence and spread of NDM-1 carbapenemase producer Enterobacteriaceae and their plasmids in the United Arab Emirates." Sonnevend A., Al Baloushi A., Ghazawi A., Hashmey R., Girgis S., Hamadeh M.B., Al Haj M., Pal T. Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ABC85 EMBL AGB57374.1.
[13]	"Inc A/C plasmids harbouring the NDM-1 metallo-beta-lactamase." Boyd D.A., Mataseje L.F., Mulvey M.R. Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: N10-2337 EMBL AEP32605.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

HQ162469 Genomic DNA. Translation: ADP20459.1.
HQ451074 Genomic DNA. Translation: ADY00041.1.
JF922606 Genomic DNA. Translation: AEH43774.1.
JF785549 Genomic DNA. Translation: AEK64842.1.
JF503991 Genomic DNA. Translation: AEN71485.1.
JF714412 Genomic DNA. Translation: AEP32605.1.
JQ364967 Genomic DNA. Translation: AFI72857.1.
JX000475 Genomic DNA. Translation: AFN73221.1.
JX296013 Genomic DNA. Translation: AFU75168.1.
KC149527 Genomic DNA. Translation: AGB57374.1.
AB604953 Genomic DNA. Translation: BAK40112.1.
AP012208 Genomic DNA. Translation: BAK69668.1.

RefSeq

YP_004765050.1. NC_015872.1.
YP_006903357.1. NC_019045.1.
YP_006939981.1. NC_018994.1.
YP_006952434.1. NC_019063.1.
YP_006953215.1. NC_019069.1.
YP_006953875.1. NC_019089.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3S0Z	X-ray	2.50	A/B	47-270	[»]

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Genome annotation databases

GeneID

11027496.
13876866.
13903469.
13905367.
13906127.
13906812.

Family and domain databases

InterPro

IPR001279. Beta-lactamas-like.
[Graphical view]

Pfam

PF00753. Lactamase_B. 1 hit.
[Graphical view]

SMART

SM00849. Lactamase_B. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

E5KIY2_ECOLX

Accession

Primary (citable) accession number: E5KIY2

Entry history

Integrated into UniProtKB/TrEMBL:	February 8, 2011
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)