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E5AMF4 (E5AMF4_BURRH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162
Ordered Locus Names:RBRH_02204 EMBL CBW76186.1
OrganismBurkholderia rhizoxinica (strain DSM 19002 / CIP 109453 / HKI 454) [Complete proteome] [HAMAP]
Taxonomic identifier882378 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia ›

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain127 – 312186ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding153 – 20957ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2831Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2851Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
E5AMF4 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: 3A7603FECBA69B9E

FASTA31634,791
        10         20         30         40         50         60 
MDLLFIADPL PQFKIYKDTT YAMMAEAARR GHVLYACEPR HLAYAGGGVE ATVRRFSIVG 

        70         80         90        100        110        120 
DHTNPGQWYD AELAENRGLG TFGAVLMRKD PPFDMEYVTS TWLLEIAERH GARIFNKPRA 

       130        140        150        160        170        180 
IRDHSEKMAI GEFARFTAPT LVTRDAARLR AFHGEHRDVI FKPLDGMGGM GVFRIREDGM 

       190        200        210        220        230        240 
NLGSTIEMLS DNGARTVMAQ RYIPQIKDGD KRILLIGGEP VPYSLARIPQ GTEIRGNLAA 

       250        260        270        280        290        300 
GGVGRAQSLA PRDREIATAL GPVLAERGLL LVGLDTIGDW LTEVNVTSPT CFREITEQTG 

       310 
FNVAAMFVDA LERAVG

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References

[1]"Complete genome sequence of Burkholderia rhizoxinica, an Endosymbiont of Rhizopus microsporus."
Lackner G., Moebius N., Partida-Martinez L., Hertweck C.
J. Bacteriol. 193:783-784(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 19002 / CIP 109453 / HKI 454.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FR687359 Genomic DNA. Translation: CBW76186.1.
RefSeqYP_004030330.1. NC_014722.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBW76186; CBW76186; RBRH_02204.
GeneID9986656.
KEGGbrh:RBRH_02204.
PATRIC45187081. VBIBurRhi170666_3300.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000265022.
KOK01920.

Enzyme and pathway databases

BioCycBRHI882378:GJIB-2724-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE5AMF4_BURRH
AccessionPrimary (citable) accession number: E5AMF4
Entry history
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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