E4ZHV7 (AMPP3_LEPMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase PEPP
EC=3.4.11.9

Alternative name(s):
Aminoacylproline aminopeptidase
Prolidase

Gene names

Name:	PEPP
ORF Names:	Lema_P059740

Organism

Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam) [Complete proteome]

Taxonomic identifier

985895 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › dothideomyceta › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Leptosphaeriaceae › Leptosphaeria › Leptosphaeria maculans complex › Leptosphaeria maculans › Leptosphaeria maculans 'brassicae' group

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides By similarity.
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Probable Xaa-Pro aminopeptidase PEPP		PRO_0000411873
Sites
	Metal binding	358	1	Manganese 2 By similarity
	Metal binding	369	1	Manganese 1 By similarity
	Metal binding	369	1	Manganese 2 By similarity
	Metal binding	492	1	Manganese 1 By similarity
	Metal binding	532	1	Manganese 1 By similarity
	Metal binding	532	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	E4ZHV7 [UniParc]. Last modified February 8, 2011. Version 1. Checksum: 5C576ABEED6145EC	FASTA	562	63,896
	10 20 30 40 50 60 MTRTMVVTVR FCTFLINVDD EWDANPWLPE QVSLDPRPPL KHRPKLNFDM SAFSSRGMRL 70 80 90 100 110 120 ARTLQRRYTR RPLPTYQQPT PFTFRILPAE DREWCSISTM AIAENYEDVL KGKYPAKEHA 130 140 150 160 170 180 RKVAKWIIEK GGDKNGTIYL EAQKQKLNED NDGEAPFRQR RYFFYLSGCE LPDSYLTYEI 190 200 210 220 230 240 PNDRLTLFIP PVEPEEVIWS GLPMSVDEAK AKYDIDDCKT TRDINAHLTS TSESAQSTIY 250 260 270 280 290 300 AIPEQVSDNI TFLSYKDKEF KQLKPAIEYC RVTKTDYEIA LIRKANEIST AAHIAVMKAA 310 320 330 340 350 360 SKAKNECELE AVFLKSCVER NAKNQAYHSI VAAGENGATL HYVNNAAPIS EQNLLLLDAG 370 380 390 400 410 420 CEVDCYASDI TRTFPIKGHF NEESLAIYKI VLDMQHQCIN ALKAGVLWDS IHELAHKIAI 430 440 450 460 470 480 KGLLDLGILK GDADAIFKAR ASVAFFPHGL GHYLGMDTHD TGGNANYADK DVMFRYLRVR 490 500 510 520 530 540 GTLPERSVIT VEPGIYFCRF IIEPYLKDEE KKQFFDEKVL EKYWSVGGVR IEDNILITKE 550 560 GIENLTPTPK EVDEITALVQ SA

References

[1]

"Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations." Rouxel T., Grandaubert J., Hane J.K., Hoede C., van de Wouw A.P., Couloux A., Dominguez V., Anthouard V., Bally P., Bourras S., Cozijnsen A.J., Ciuffetti L.M., Degrave A., Dilmaghani A., Duret L., Fudal I., Goodwin S.B., Gout L. , Glaser N., Linglin J., Kema G.H.J., Lapalu N., Lawrence C.B., May K., Meyer M., Ollivier B., Poulain J., Schoch C.L., Simon A., Spatafora J.W., Stachowiak A., Turgeon B.G., Tyler B.M., Vincent D., Weissenbach J., Amselem J., Quesneville H., Oliver R.P., Wincker P., Balesdent M.-H., Howlett B.J. Nat. Commun. 2:202-202(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FP929065 Genomic DNA. Translation: CBX90940.1.
RefSeq	XP_003834305.1. XM_003834257.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CBX90940; CBX90940; LEMA_P059740.1.
GeneID	13284487.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPP3_LEPMJ

Accession

Primary (citable) accession number: E4ZHV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	February 8, 2011
Last modified:	March 6, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program