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Protein

Isocitrate dehydrogenase [NADP]

Gene

REQ_34420

Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Binding sitei82NADPUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Sitei139Critical for catalysisUniRule annotation1
Sitei212Critical for catalysisUniRule annotation1
Metal bindingi252Magnesium or manganeseUniRule annotation1
Binding sitei260NADPUniRule annotation1
Metal bindingi275Magnesium or manganeseUniRule annotation1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPUniRule annotation3
Nucleotide bindingi310 – 315NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciREQU685727:GHKP-3320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:REQ_34420Imported
OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
Taxonomic identifieri685727 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000006892 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi685727.REQ_34420.

Structurei

3D structure databases

ProteinModelPortaliE4WKH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 396Iso_dhInterPro annotationAdd BLAST388

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D5N. Bacteria.
COG0538. LUCA.
HOGENOMiHOG000019858.
KOiK00031.
OMAiTVQRHYY.
OrthoDBiPOG091H0JP0.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E4WKH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKVEGKV VELDGDEMTR IIWQFIKDKL IHPYLDVDLE YYDLGIEHRD
60 70 80 90 100
ATDDQVTIDA AEAIKKHGVG VKCATITPDE ARVEEFGLKK MWRSPNGTIR
110 120 130 140 150
NILGGTIFRA PILISNVPRL VPGWTKPVIV GRHAFGDQYR ATDFKAPGAG
160 170 180 190 200
TVTITYTPDD GSEPIQHEVC RLPEDGGVVM GMYNYKKSIQ DFARASLSYG
210 220 230 240 250
LQQNYPVYLS TKNTILKAYD GMFKDEFQRI YEDEFKAEFD AAGLHYEHRL
260 270 280 290 300
IDDMVASSLK WEGGYVWACK NYDGDVQSDT VAQGYGSLGL MTSVLMTPDG
310 320 330 340 350
RTVEAEAAHG TVTRHYRQHQ QGKPTSTNPI ASIFAWTRGL EHRGKLDNTP
360 370 380 390 400
ELIEFAQQLE DVVIKTVENG QMTKDLALLV GPDQAWQTTE EFLATLDENL

QKARA
Length:405
Mass (Da):45,431
Last modified:February 8, 2011 - v1
Checksum:i8D7179D70CD0CF22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN563149 Genomic DNA. Translation: CBH49437.1.
RefSeqiWP_005518554.1. NC_014659.1.

Genome annotation databases

EnsemblBacteriaiCBH49437; CBH49437; REQ_34420.
KEGGireq:REQ_34420.
PATRICi42664361. VBIRhoEqu141084_3444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN563149 Genomic DNA. Translation: CBH49437.1.
RefSeqiWP_005518554.1. NC_014659.1.

3D structure databases

ProteinModelPortaliE4WKH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi685727.REQ_34420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBH49437; CBH49437; REQ_34420.
KEGGireq:REQ_34420.
PATRICi42664361. VBIRhoEqu141084_3444.

Phylogenomic databases

eggNOGiENOG4105D5N. Bacteria.
COG0538. LUCA.
HOGENOMiHOG000019858.
KOiK00031.
OMAiTVQRHYY.
OrthoDBiPOG091H0JP0.

Enzyme and pathway databases

BioCyciREQU685727:GHKP-3320-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE4WKH8_RHOE1
AccessioniPrimary (citable) accession number: E4WKH8
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: November 2, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.