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E4WKH8 (E4WKH8_RHOE1) Unreviewed, UniProtKB/TrEMBL

Last modified October 16, 2013. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108

EC=1.1.1.42 PIRNR PIRNR000108
Gene names
Ordered Locus Names:REQ_34420 EMBL CBH49437.1
OrganismRhodococcus equi (strain 103S) (Corynebacterium equi) [Complete proteome] [HAMAP] EMBL CBH49437.1
Taxonomic identifier685727 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding75 – 773NADP By similarity
Nucleotide binding310 – 3156NADP By similarity
Region94 – 1007Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding2521Magnesium or manganese By similarity PIRSR PIRSR000108-3
Metal binding2751Magnesium or manganese By similarity PIRSR PIRSR000108-3
Binding site771Substrate By similarity PIRSR PIRSR000108-2
Binding site821NADP By similarity
Binding site1091Substrate By similarity PIRSR PIRSR000108-2
Binding site1321Substrate By similarity PIRSR PIRSR000108-2
Binding site2601NADP By similarity
Binding site3281NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1391Critical for catalysis By similarity PIRSR PIRSR000108-1
Site2121Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence LengthMass (Da)Tools
E4WKH8 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: 8D7179D70CD0CF22

FASTA40545,431
        10         20         30         40         50         60 
MSKIKVEGKV VELDGDEMTR IIWQFIKDKL IHPYLDVDLE YYDLGIEHRD ATDDQVTIDA 

        70         80         90        100        110        120 
AEAIKKHGVG VKCATITPDE ARVEEFGLKK MWRSPNGTIR NILGGTIFRA PILISNVPRL 

       130        140        150        160        170        180 
VPGWTKPVIV GRHAFGDQYR ATDFKAPGAG TVTITYTPDD GSEPIQHEVC RLPEDGGVVM 

       190        200        210        220        230        240 
GMYNYKKSIQ DFARASLSYG LQQNYPVYLS TKNTILKAYD GMFKDEFQRI YEDEFKAEFD 

       250        260        270        280        290        300 
AAGLHYEHRL IDDMVASSLK WEGGYVWACK NYDGDVQSDT VAQGYGSLGL MTSVLMTPDG 

       310        320        330        340        350        360 
RTVEAEAAHG TVTRHYRQHQ QGKPTSTNPI ASIFAWTRGL EHRGKLDNTP ELIEFAQQLE 

       370        380        390        400 
DVVIKTVENG QMTKDLALLV GPDQAWQTTE EFLATLDENL QKARA 

« Hide

References

[1]"The genome of a pathogenic rhodococcus: cooptive virulence underpinned by key gene acquisitions."
Letek M., Gonzalez P., Macarthur I., Rodriguez H., Freeman T.C., Valero-Rello A., Blanco M., Buckley T., Cherevach I., Fahey R., Hapeshi A., Holdstock J., Leadon D., Navas J., Ocampo A., Quail M.A., Sanders M., Scortti M.M. expand/collapse author list , Prescott J.F., Fogarty U., Meijer W.G., Parkhill J., Bentley S.D., Vazquez-Boland J.A.
PLoS Genet. 6:E1001145-E1001145(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 103S.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN563149 Genomic DNA. Translation: CBH49437.1.
RefSeqYP_004008116.1. NC_014659.1.

3D structure databases

ProteinModelPortalE4WKH8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBH49437; CBH49437; REQ_34420.
GeneID9965784.
KEGGreq:REQ_34420.
PATRIC42664361. VBIRhoEqu141084_3444.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000019858.
KOK00031.
OMAQHQQGKP.

Enzyme and pathway databases

BioCycREQU685727:GHKP-3329-MONOMER.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE4WKH8_RHOE1
AccessionPrimary (citable) accession number: E4WKH8
Entry history
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: October 16, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)