Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

E4WKH8

- E4WKH8_RHOE1

UniProt

E4WKH8 - E4WKH8_RHOE1

Protein

Isocitrate dehydrogenase [NADP]

Gene

REQ_34420

Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (08 Feb 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateUniRule annotation
    Binding sitei82 – 821NADPUniRule annotation
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Sitei139 – 1391Critical for catalysisUniRule annotation
    Sitei212 – 2121Critical for catalysisUniRule annotation
    Metal bindingi252 – 2521Magnesium or manganese
    Binding sitei260 – 2601NADPUniRule annotation
    Metal bindingi275 – 2751Magnesium or manganese
    Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADPUniRule annotation
    Nucleotide bindingi310 – 3156NADPUniRule annotation

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Enzyme and pathway databases

    BioCyciREQU685727:GHKP-3329-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    Ordered Locus Names:REQ_34420Imported
    OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
    Taxonomic identifieri685727 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000006892: Chromosome

    Structurei

    3D structure databases

    ProteinModelPortaliE4WKH8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000019858.
    KOiK00031.
    OMAiFKDEFQR.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E4WKH8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIKVEGKV VELDGDEMTR IIWQFIKDKL IHPYLDVDLE YYDLGIEHRD    50
    ATDDQVTIDA AEAIKKHGVG VKCATITPDE ARVEEFGLKK MWRSPNGTIR 100
    NILGGTIFRA PILISNVPRL VPGWTKPVIV GRHAFGDQYR ATDFKAPGAG 150
    TVTITYTPDD GSEPIQHEVC RLPEDGGVVM GMYNYKKSIQ DFARASLSYG 200
    LQQNYPVYLS TKNTILKAYD GMFKDEFQRI YEDEFKAEFD AAGLHYEHRL 250
    IDDMVASSLK WEGGYVWACK NYDGDVQSDT VAQGYGSLGL MTSVLMTPDG 300
    RTVEAEAAHG TVTRHYRQHQ QGKPTSTNPI ASIFAWTRGL EHRGKLDNTP 350
    ELIEFAQQLE DVVIKTVENG QMTKDLALLV GPDQAWQTTE EFLATLDENL 400
    QKARA 405
    Length:405
    Mass (Da):45,431
    Last modified:February 8, 2011 - v1
    Checksum:i8D7179D70CD0CF22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN563149 Genomic DNA. Translation: CBH49437.1.
    RefSeqiWP_005518554.1. NC_014659.1.
    YP_004008116.1. NC_014659.1.

    Genome annotation databases

    EnsemblBacteriaiCBH49437; CBH49437; REQ_34420.
    GeneIDi9965784.
    KEGGireq:REQ_34420.
    PATRICi42664361. VBIRhoEqu141084_3444.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN563149 Genomic DNA. Translation: CBH49437.1 .
    RefSeqi WP_005518554.1. NC_014659.1.
    YP_004008116.1. NC_014659.1.

    3D structure databases

    ProteinModelPortali E4WKH8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBH49437 ; CBH49437 ; REQ_34420 .
    GeneIDi 9965784.
    KEGGi req:REQ_34420.
    PATRICi 42664361. VBIRhoEqu141084_3444.

    Phylogenomic databases

    HOGENOMi HOG000019858.
    KOi K00031.
    OMAi FKDEFQR.

    Enzyme and pathway databases

    BioCyci REQU685727:GHKP-3329-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 103SImported.

    Entry informationi

    Entry nameiE4WKH8_RHOE1
    AccessioniPrimary (citable) accession number: E4WKH8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 8, 2011
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3