Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E4WJM4 (E4WJM4_RHOE1) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:REQ_31280 EMBL CBH49132.1
OrganismRhodococcus equi (strain 103S) (Corynebacterium equi) [Complete proteome] [HAMAP] EMBL CBH49132.1
Taxonomic identifier685727 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain151 – 358208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding179 – 23456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3131Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3251Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3251Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3271Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E4WJM4 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: ACB66F757358C103

FASTA36838,962
        10         20         30         40         50         60 
MNNPRTRVAV VFGGRSNEHS VSCVSAGSVL RNLDPDRYEV VPIGITTDGA WVLGSTDPAD 

        70         80         90        100        110        120 
LAIRDRSMPT VDAGGKALTL TADPTRAGAV VALDDKQAGQ ALASVDVVFP VLHGAYGEDG 

       130        140        150        160        170        180 
TIQGLLELAG IPYVGPGVLA SAAGMDKEFT KKLLAAEGLP VGFQVVLRPG TSTVTDEQKQ 

       190        200        210        220        230        240 
QLGLPVFVKP ARGGSSIGIT RVADWSQFDA AVAVARSHDP KVIVESAIVG REVECGVLEF 

       250        260        270        280        290        300 
PDGDVRASVV AEIRMPDTDG DESAFYDFDT KYLDDVCEFD VPAKLDDDVS EQIRALAVRA 

       310        320        330        340        350        360 
FRALDCQGLA RVDFFVTADG PVINEINTMP GFTSISMYPR MWQAAGVEYE TLVSTLVDTA 


VARGTGLR 

« Hide

References

[1]"The genome of a pathogenic rhodococcus: cooptive virulence underpinned by key gene acquisitions."
Letek M., Gonzalez P., Macarthur I., Rodriguez H., Freeman T.C., Valero-Rello A., Blanco M., Buckley T., Cherevach I., Fahey R., Hapeshi A., Holdstock J., Leadon D., Navas J., Ocampo A., Quail M.A., Sanders M., Scortti M.M. expand/collapse author list , Prescott J.F., Fogarty U., Meijer W.G., Parkhill J., Bentley S.D., Vazquez-Boland J.A.
PLoS Genet. 6:E1001145-E1001145(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 103S.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN563149 Genomic DNA. Translation: CBH49132.1.
RefSeqYP_004007812.1. NC_014659.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBH49132; CBH49132; REQ_31280.
GeneID9965554.
KEGGreq:REQ_31280.
PATRIC42663713. VBIRhoEqu141084_3125.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.

Enzyme and pathway databases

BioCycREQU685727:GHKP-3020-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE4WJM4_RHOE1
AccessionPrimary (citable) accession number: E4WJM4
Entry history
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)