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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation
  • Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotationSAAS annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
Ordered Locus Names:REQ_21680Imported
OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
Taxonomic identifieri685727 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000006892 Componenti: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi685727.REQ_21680.

Structurei

3D structure databases

ProteinModelPortaliE4WH16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 333ATP-graspInterPro annotationAdd BLAST196
Domaini694 – 885ATP-graspInterPro annotationAdd BLAST192

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 407Carboxyphosphate synthetic domainUniRule annotationAdd BLAST407
Regioni967 – 1115Allosteric domainUniRule annotationAdd BLAST149

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CU6. Bacteria.
COG0458. LUCA.
HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiPOG091H01IP.

Family and domain databases

CDDicd01424. MGS_CPS_II. 1 hit.
Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 1 hit.
3.40.50.1380. 1 hit.
HAMAPiMF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR033937. MGS_CPS_CarB.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
PRINTSiPR00098. CPSASE.
SMARTiView protein in SMART
SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.

Sequencei

Sequence statusi: Complete.

E4WH16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRTDLEHI LVIGSGPIVI GQACEFDYSG TQACRVLREE GLRVSLVNSN
60 70 80 90 100
PATIMTDPEF ADSTYVEPIT AEFVEKVIAR EAANGNKIDA VLATLGGQTA
110 120 130 140 150
LNTAVALHEQ GILEKYDVEL IGADFDAIQR GEDRQKFKDI VEKCGGESAR
160 170 180 190 200
SRVCYTMEEV KATVAELGYP VVVRPSFTMG GLGSGMAYDE VDLERIAGGG
210 220 230 240 250
LAASPTANVL IEESILGWKE YELELMRDGR DNVVIVCSIE NVDPVGVHTG
260 270 280 290 300
DSVTVAPAMT LTDREYQAMR DLSIAILREV GVDTGGCNIQ FAMDPKDGRL
310 320 330 340 350
VVIEMNPRVS RSSALASKAT GYPIAKMAAK LAIGYTLDEI VNDITKETPA
360 370 380 390 400
CFEPTLDYVV VKAPRFAFEK FPGADGTLTT TMKSVGEAMS IGRNFTEAFG
410 420 430 440 450
KVLRSLETKR AGYWTGPEVE GTLDELLEAI KVPTDGRMYQ IAQAFELGAT
460 470 480 490 500
LEQLFDATAI DPWFLDQMQQ IVELGAEVRS AETFGATELR FAKHHGFSDR
510 520 530 540 550
QIAALRPQQF GEGQAGEDAV RALRDELNVH PVYKTVDTCA AEFEAKTPYH
560 570 580 590 600
YSTYELDPEA ETEVAPQTER PKVLILGSGP NRIGQGIEFD YSCVHAAQTL
610 620 630 640 650
SEAGYETVMV NCNPETVSTD YDTADRLYFE PLTFEDVLEV YRAETLSGTV
660 670 680 690 700
AGVIVQLGGQ TPLGLAKRLK AAGVPIVGTS PEAIDLAEDR GEFGRVLTEA
710 720 730 740 750
GLPAPKFGTA TTFEGAREIA SGIGYPVLVR PSYVLGGRGM EIVYDEKALE
760 770 780 790 800
SYISRATEIS DDRPVLVDRF LEDAVEIDVD ALCDGTEVYL GGVMEHIEEA
810 820 830 840 850
GIHSGDSACA LPPITLGRAD LENVRRSTEA LAKGIGVKGL LNVQYALKDD
860 870 880 890 900
ILYVLEANPR ASRTVPFVSK ATAVQLAKAC ARVMLGESIA DLRAGGILPA
910 920 930 940 950
EGDGGHAPID APVAVKEAVL PFNRFRRADG TGIDTLLSPE MKSTGEVMGI
960 970 980 990 1000
DADFGTAFAK SQTAAYGSLP TSGSVFVSVA NKDKRSLIFP VKRLADLGFR
1010 1020 1030 1040 1050
ILATEGTADV LRRNGITCEE VHKQSGENLP EGARTIVDQI KDGEVDMVIN
1060 1070 1080 1090 1100
TPYGNSGPRV DGYEIRSAAV SKSVPCITTV QGASAAVQGI EAAIRGDIGV
1110
QSLQELHTRL RDQQQ
Length:1,115
Mass (Da):119,924
Last modified:February 8, 2011 - v1
Checksum:i85E7D80ECA0C1880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN563149 Genomic DNA. Translation: CBH48221.1.
RefSeqiWP_005513444.1. NC_014659.1.

Genome annotation databases

EnsemblBacteriaiCBH48221; CBH48221; REQ_21680.
GeneIDi32491691.
KEGGireq:REQ_21680.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiE4WH16_RHOE1
AccessioniPrimary (citable) accession number: E4WH16
Entry historyiIntegrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: July 5, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported