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E4WH16

- E4WH16_RHOE1

UniProt

E4WH16 - E4WH16_RHOE1

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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Note: Binds 4 magnesium or manganese ions per subunit.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi290 – 2901Magnesium or manganese 1UniRule annotation
Metal bindingi304 – 3041Magnesium or manganese 1UniRule annotation
Metal bindingi304 – 3041Magnesium or manganese 2UniRule annotation
Metal bindingi306 – 3061Magnesium or manganese 2UniRule annotation
Metal bindingi844 – 8441Magnesium or manganese 3UniRule annotation
Metal bindingi856 – 8561Magnesium or manganese 3UniRule annotation
Metal bindingi856 – 8561Magnesium or manganese 4UniRule annotation
Metal bindingi858 – 8581Magnesium or manganese 4UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 22158ATPUniRule annotationAdd
BLAST
Nucleotide bindingi720 – 77758ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisUniRule annotationSAAS annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciREQU685727:GHKP-2094-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
Ordered Locus Names:REQ_21680Imported
OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
Taxonomic identifieri685727 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000006892: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE4WH16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 333196ATP-grasp 1UniRule annotationAdd
BLAST
Domaini694 – 885192ATP-grasp 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 407407Carboxyphosphate synthetic domainUniRule annotationAdd
BLAST
Regioni408 – 564157Oligomerization domainUniRule annotationAdd
BLAST
Regioni565 – 966402Carbamoyl phosphate synthetic domainUniRule annotationAdd
BLAST
Regioni967 – 1115149Allosteric domainUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CarB family.UniRule annotation
Contains 2 ATP-grasp domains.UniRule annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiTFIDKWF.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E4WH16-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRRTDLEHI LVIGSGPIVI GQACEFDYSG TQACRVLREE GLRVSLVNSN
60 70 80 90 100
PATIMTDPEF ADSTYVEPIT AEFVEKVIAR EAANGNKIDA VLATLGGQTA
110 120 130 140 150
LNTAVALHEQ GILEKYDVEL IGADFDAIQR GEDRQKFKDI VEKCGGESAR
160 170 180 190 200
SRVCYTMEEV KATVAELGYP VVVRPSFTMG GLGSGMAYDE VDLERIAGGG
210 220 230 240 250
LAASPTANVL IEESILGWKE YELELMRDGR DNVVIVCSIE NVDPVGVHTG
260 270 280 290 300
DSVTVAPAMT LTDREYQAMR DLSIAILREV GVDTGGCNIQ FAMDPKDGRL
310 320 330 340 350
VVIEMNPRVS RSSALASKAT GYPIAKMAAK LAIGYTLDEI VNDITKETPA
360 370 380 390 400
CFEPTLDYVV VKAPRFAFEK FPGADGTLTT TMKSVGEAMS IGRNFTEAFG
410 420 430 440 450
KVLRSLETKR AGYWTGPEVE GTLDELLEAI KVPTDGRMYQ IAQAFELGAT
460 470 480 490 500
LEQLFDATAI DPWFLDQMQQ IVELGAEVRS AETFGATELR FAKHHGFSDR
510 520 530 540 550
QIAALRPQQF GEGQAGEDAV RALRDELNVH PVYKTVDTCA AEFEAKTPYH
560 570 580 590 600
YSTYELDPEA ETEVAPQTER PKVLILGSGP NRIGQGIEFD YSCVHAAQTL
610 620 630 640 650
SEAGYETVMV NCNPETVSTD YDTADRLYFE PLTFEDVLEV YRAETLSGTV
660 670 680 690 700
AGVIVQLGGQ TPLGLAKRLK AAGVPIVGTS PEAIDLAEDR GEFGRVLTEA
710 720 730 740 750
GLPAPKFGTA TTFEGAREIA SGIGYPVLVR PSYVLGGRGM EIVYDEKALE
760 770 780 790 800
SYISRATEIS DDRPVLVDRF LEDAVEIDVD ALCDGTEVYL GGVMEHIEEA
810 820 830 840 850
GIHSGDSACA LPPITLGRAD LENVRRSTEA LAKGIGVKGL LNVQYALKDD
860 870 880 890 900
ILYVLEANPR ASRTVPFVSK ATAVQLAKAC ARVMLGESIA DLRAGGILPA
910 920 930 940 950
EGDGGHAPID APVAVKEAVL PFNRFRRADG TGIDTLLSPE MKSTGEVMGI
960 970 980 990 1000
DADFGTAFAK SQTAAYGSLP TSGSVFVSVA NKDKRSLIFP VKRLADLGFR
1010 1020 1030 1040 1050
ILATEGTADV LRRNGITCEE VHKQSGENLP EGARTIVDQI KDGEVDMVIN
1060 1070 1080 1090 1100
TPYGNSGPRV DGYEIRSAAV SKSVPCITTV QGASAAVQGI EAAIRGDIGV
1110
QSLQELHTRL RDQQQ
Length:1,115
Mass (Da):119,924
Last modified:February 8, 2011 - v1
Checksum:i85E7D80ECA0C1880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN563149 Genomic DNA. Translation: CBH48221.1.
RefSeqiWP_005513444.1. NC_014659.1.
YP_004006905.1. NC_014659.1.

Genome annotation databases

EnsemblBacteriaiCBH48221; CBH48221; REQ_21680.
GeneIDi9966933.
KEGGireq:REQ_21680.
PATRICi42661759. VBIRhoEqu141084_2164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN563149 Genomic DNA. Translation: CBH48221.1 .
RefSeqi WP_005513444.1. NC_014659.1.
YP_004006905.1. NC_014659.1.

3D structure databases

ProteinModelPortali E4WH16.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBH48221 ; CBH48221 ; REQ_21680 .
GeneIDi 9966933.
KEGGi req:REQ_21680.
PATRICi 42661759. VBIRhoEqu141084_2164.

Phylogenomic databases

HOGENOMi HOG000234582.
KOi K01955.
OMAi TFIDKWF.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00171 .
UPA00070 ; UER00115 .
BioCyci REQU685727:GHKP-2094-MONOMER.

Family and domain databases

Gene3Di 1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPi MF_01210_B. CPSase_L_chain_B.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PRINTSi PR00098. CPSASE.
SMARTi SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
PROSITEi PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 103SImported.

Entry informationi

Entry nameiE4WH16_RHOE1
AccessioniPrimary (citable) accession number: E4WH16
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: November 26, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3