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E4WH16

- E4WH16_RHOE1

UniProt

E4WH16 - E4WH16_RHOE1

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Protein

Carbamoyl-phosphate synthase large chain

Gene
carB, REQ_21680
Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotations

Cofactori

Binds 4 magnesium or manganese ions per subunit By similarity.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi290 – 2901Magnesium or manganese 1 By similarityUniRule annotation
Metal bindingi304 – 3041Magnesium or manganese 1 By similarityUniRule annotation
Metal bindingi304 – 3041Magnesium or manganese 2 By similarityUniRule annotation
Metal bindingi306 – 3061Magnesium or manganese 2 By similarityUniRule annotation
Metal bindingi844 – 8441Magnesium or manganese 3 By similarityUniRule annotation
Metal bindingi856 – 8561Magnesium or manganese 3 By similarityUniRule annotation
Metal bindingi856 – 8561Magnesium or manganese 4 By similarityUniRule annotation
Metal bindingi858 – 8581Magnesium or manganese 4 By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 22158ATP By similarityUniRule annotationAdd
BLAST
Nucleotide bindingi720 – 77758ATP By similarityUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisUniRule annotationSAAS annotations, Pyrimidine biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotations, ManganeseUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciREQU685727:GHKP-2094-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Gene namesi
Name:carBUniRule annotationImported
Ordered Locus Names:REQ_21680Imported
OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
Taxonomic identifieri685727 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000006892: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliE4WH16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 333196ATP-grasp 1 By similarityUniRule annotationAdd
BLAST
Domaini694 – 885192ATP-grasp 2 By similarityUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 407407Carboxyphosphate synthetic domain By similarityUniRule annotationAdd
BLAST
Regioni408 – 564157Oligomerization domain By similarityUniRule annotationAdd
BLAST
Regioni565 – 966402Carbamoyl phosphate synthetic domain By similarityUniRule annotationAdd
BLAST
Regioni967 – 1115149Allosteric domain By similarityUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CarB family.UniRule annotation
Contains 2 ATP-grasp domains.UniRule annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiTFIDKWF.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E4WH16-1 [UniParc]FASTAAdd to Basket

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MPRRTDLEHI LVIGSGPIVI GQACEFDYSG TQACRVLREE GLRVSLVNSN     50
PATIMTDPEF ADSTYVEPIT AEFVEKVIAR EAANGNKIDA VLATLGGQTA 100
LNTAVALHEQ GILEKYDVEL IGADFDAIQR GEDRQKFKDI VEKCGGESAR 150
SRVCYTMEEV KATVAELGYP VVVRPSFTMG GLGSGMAYDE VDLERIAGGG 200
LAASPTANVL IEESILGWKE YELELMRDGR DNVVIVCSIE NVDPVGVHTG 250
DSVTVAPAMT LTDREYQAMR DLSIAILREV GVDTGGCNIQ FAMDPKDGRL 300
VVIEMNPRVS RSSALASKAT GYPIAKMAAK LAIGYTLDEI VNDITKETPA 350
CFEPTLDYVV VKAPRFAFEK FPGADGTLTT TMKSVGEAMS IGRNFTEAFG 400
KVLRSLETKR AGYWTGPEVE GTLDELLEAI KVPTDGRMYQ IAQAFELGAT 450
LEQLFDATAI DPWFLDQMQQ IVELGAEVRS AETFGATELR FAKHHGFSDR 500
QIAALRPQQF GEGQAGEDAV RALRDELNVH PVYKTVDTCA AEFEAKTPYH 550
YSTYELDPEA ETEVAPQTER PKVLILGSGP NRIGQGIEFD YSCVHAAQTL 600
SEAGYETVMV NCNPETVSTD YDTADRLYFE PLTFEDVLEV YRAETLSGTV 650
AGVIVQLGGQ TPLGLAKRLK AAGVPIVGTS PEAIDLAEDR GEFGRVLTEA 700
GLPAPKFGTA TTFEGAREIA SGIGYPVLVR PSYVLGGRGM EIVYDEKALE 750
SYISRATEIS DDRPVLVDRF LEDAVEIDVD ALCDGTEVYL GGVMEHIEEA 800
GIHSGDSACA LPPITLGRAD LENVRRSTEA LAKGIGVKGL LNVQYALKDD 850
ILYVLEANPR ASRTVPFVSK ATAVQLAKAC ARVMLGESIA DLRAGGILPA 900
EGDGGHAPID APVAVKEAVL PFNRFRRADG TGIDTLLSPE MKSTGEVMGI 950
DADFGTAFAK SQTAAYGSLP TSGSVFVSVA NKDKRSLIFP VKRLADLGFR 1000
ILATEGTADV LRRNGITCEE VHKQSGENLP EGARTIVDQI KDGEVDMVIN 1050
TPYGNSGPRV DGYEIRSAAV SKSVPCITTV QGASAAVQGI EAAIRGDIGV 1100
QSLQELHTRL RDQQQ 1115
Length:1,115
Mass (Da):119,924
Last modified:February 8, 2011 - v1
Checksum:i85E7D80ECA0C1880
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN563149 Genomic DNA. Translation: CBH48221.1.
RefSeqiWP_005513444.1. NC_014659.1.
YP_004006905.1. NC_014659.1.

Genome annotation databases

EnsemblBacteriaiCBH48221; CBH48221; REQ_21680.
GeneIDi9966933.
KEGGireq:REQ_21680.
PATRICi42661759. VBIRhoEqu141084_2164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN563149 Genomic DNA. Translation: CBH48221.1 .
RefSeqi WP_005513444.1. NC_014659.1.
YP_004006905.1. NC_014659.1.

3D structure databases

ProteinModelPortali E4WH16.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBH48221 ; CBH48221 ; REQ_21680 .
GeneIDi 9966933.
KEGGi req:REQ_21680.
PATRICi 42661759. VBIRhoEqu141084_2164.

Phylogenomic databases

HOGENOMi HOG000234582.
KOi K01955.
OMAi TFIDKWF.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00171 .
UPA00070 ; UER00115 .
BioCyci REQU685727:GHKP-2094-MONOMER.

Family and domain databases

Gene3Di 1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPi MF_01210_B. CPSase_L_chain_B.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PRINTSi PR00098. CPSASE.
SMARTi SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
PROSITEi PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 103S.

Entry informationi

Entry nameiE4WH16_RHOE1
AccessioniPrimary (citable) accession number: E4WH16
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: September 3, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi