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E4WH16

- E4WH16_RHOE1

UniProt

E4WH16 - E4WH16_RHOE1

Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (08 Feb 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

    Cofactori

    Binds 4 magnesium or manganese ions per subunit.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi290 – 2901Magnesium or manganese 1UniRule annotation
    Metal bindingi304 – 3041Magnesium or manganese 1UniRule annotation
    Metal bindingi304 – 3041Magnesium or manganese 2UniRule annotation
    Metal bindingi306 – 3061Magnesium or manganese 2UniRule annotation
    Metal bindingi844 – 8441Magnesium or manganese 3UniRule annotation
    Metal bindingi856 – 8561Magnesium or manganese 3UniRule annotation
    Metal bindingi856 – 8561Magnesium or manganese 4UniRule annotation
    Metal bindingi858 – 8581Magnesium or manganese 4UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi164 – 22158ATPUniRule annotationAdd
    BLAST
    Nucleotide bindingi720 – 77758ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    2. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotation

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesisUniRule annotationSAAS annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciREQU685727:GHKP-2094-MONOMER.
    UniPathwayiUPA00068; UER00171.
    UPA00070; UER00115.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
    Alternative name(s):
    Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
    Gene namesi
    Name:carBUniRule annotationImported
    Ordered Locus Names:REQ_21680Imported
    OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
    Taxonomic identifieri685727 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000006892: Chromosome

    Interactioni

    Subunit structurei

    Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliE4WH16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 333196ATP-grasp 1UniRule annotationAdd
    BLAST
    Domaini694 – 885192ATP-grasp 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 407407Carboxyphosphate synthetic domainUniRule annotationAdd
    BLAST
    Regioni408 – 564157Oligomerization domainUniRule annotationAdd
    BLAST
    Regioni565 – 966402Carbamoyl phosphate synthetic domainUniRule annotationAdd
    BLAST
    Regioni967 – 1115149Allosteric domainUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CarB family.UniRule annotation
    Contains 2 ATP-grasp domains.UniRule annotation

    Keywords - Domaini

    RepeatUniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000234582.
    KOiK01955.
    OMAiTFIDKWF.

    Family and domain databases

    Gene3Di1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    HAMAPiMF_01210_B. CPSase_L_chain_B.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PRINTSiPR00098. CPSASE.
    SMARTiSM01096. CPSase_L_D3. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48108. SSF48108. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E4WH16-1 [UniParc]FASTAAdd to Basket

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    MPRRTDLEHI LVIGSGPIVI GQACEFDYSG TQACRVLREE GLRVSLVNSN     50
    PATIMTDPEF ADSTYVEPIT AEFVEKVIAR EAANGNKIDA VLATLGGQTA 100
    LNTAVALHEQ GILEKYDVEL IGADFDAIQR GEDRQKFKDI VEKCGGESAR 150
    SRVCYTMEEV KATVAELGYP VVVRPSFTMG GLGSGMAYDE VDLERIAGGG 200
    LAASPTANVL IEESILGWKE YELELMRDGR DNVVIVCSIE NVDPVGVHTG 250
    DSVTVAPAMT LTDREYQAMR DLSIAILREV GVDTGGCNIQ FAMDPKDGRL 300
    VVIEMNPRVS RSSALASKAT GYPIAKMAAK LAIGYTLDEI VNDITKETPA 350
    CFEPTLDYVV VKAPRFAFEK FPGADGTLTT TMKSVGEAMS IGRNFTEAFG 400
    KVLRSLETKR AGYWTGPEVE GTLDELLEAI KVPTDGRMYQ IAQAFELGAT 450
    LEQLFDATAI DPWFLDQMQQ IVELGAEVRS AETFGATELR FAKHHGFSDR 500
    QIAALRPQQF GEGQAGEDAV RALRDELNVH PVYKTVDTCA AEFEAKTPYH 550
    YSTYELDPEA ETEVAPQTER PKVLILGSGP NRIGQGIEFD YSCVHAAQTL 600
    SEAGYETVMV NCNPETVSTD YDTADRLYFE PLTFEDVLEV YRAETLSGTV 650
    AGVIVQLGGQ TPLGLAKRLK AAGVPIVGTS PEAIDLAEDR GEFGRVLTEA 700
    GLPAPKFGTA TTFEGAREIA SGIGYPVLVR PSYVLGGRGM EIVYDEKALE 750
    SYISRATEIS DDRPVLVDRF LEDAVEIDVD ALCDGTEVYL GGVMEHIEEA 800
    GIHSGDSACA LPPITLGRAD LENVRRSTEA LAKGIGVKGL LNVQYALKDD 850
    ILYVLEANPR ASRTVPFVSK ATAVQLAKAC ARVMLGESIA DLRAGGILPA 900
    EGDGGHAPID APVAVKEAVL PFNRFRRADG TGIDTLLSPE MKSTGEVMGI 950
    DADFGTAFAK SQTAAYGSLP TSGSVFVSVA NKDKRSLIFP VKRLADLGFR 1000
    ILATEGTADV LRRNGITCEE VHKQSGENLP EGARTIVDQI KDGEVDMVIN 1050
    TPYGNSGPRV DGYEIRSAAV SKSVPCITTV QGASAAVQGI EAAIRGDIGV 1100
    QSLQELHTRL RDQQQ 1115
    Length:1,115
    Mass (Da):119,924
    Last modified:February 8, 2011 - v1
    Checksum:i85E7D80ECA0C1880
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN563149 Genomic DNA. Translation: CBH48221.1.
    RefSeqiWP_005513444.1. NC_014659.1.
    YP_004006905.1. NC_014659.1.

    Genome annotation databases

    EnsemblBacteriaiCBH48221; CBH48221; REQ_21680.
    GeneIDi9966933.
    KEGGireq:REQ_21680.
    PATRICi42661759. VBIRhoEqu141084_2164.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN563149 Genomic DNA. Translation: CBH48221.1 .
    RefSeqi WP_005513444.1. NC_014659.1.
    YP_004006905.1. NC_014659.1.

    3D structure databases

    ProteinModelPortali E4WH16.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBH48221 ; CBH48221 ; REQ_21680 .
    GeneIDi 9966933.
    KEGGi req:REQ_21680.
    PATRICi 42661759. VBIRhoEqu141084_2164.

    Phylogenomic databases

    HOGENOMi HOG000234582.
    KOi K01955.
    OMAi TFIDKWF.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00171 .
    UPA00070 ; UER00115 .
    BioCyci REQU685727:GHKP-2094-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    HAMAPi MF_01210_B. CPSase_L_chain_B.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00098. CPSASE.
    SMARTi SM01096. CPSase_L_D3. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48108. SSF48108. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 103SImported.

    Entry informationi

    Entry nameiE4WH16_RHOE1
    AccessioniPrimary (citable) accession number: E4WH16
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 8, 2011
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3