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E4W8B1

- E4W8B1_RHOE1

UniProt

E4W8B1 - E4W8B1_RHOE1

Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (08 Feb 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotationSAAS annotation

    Catalytic activityi

    Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 thiamine pyrophosphate per subunit.UniRule annotationSAAS annotation
    Magnesium or manganese.UniRule annotationSAAS annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. menaquinone biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    TransferaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Menaquinone biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Thiamine pyrophosphateUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciREQU685727:GHKP-3581-MONOMER.
    UniPathwayiUPA00079; UER00164.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
    Short name:
    SEPHCHC synthaseUniRule annotation
    Alternative name(s):
    Menaquinone biosynthesis protein MenDUniRule annotation
    Gene namesi
    Name:menDUniRule annotationImported
    Ordered Locus Names:REQ_36990Imported
    OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
    Taxonomic identifieri685727 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000006892: Chromosome

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000218359.
    KOiK02551.
    OMAiWRSAVCR.

    Family and domain databases

    Gene3Di3.40.50.970. 2 hits.
    HAMAPiMF_01659. MenD.
    InterProiIPR004433. MenaQ_synth_MenD.
    IPR029061. THDP-binding.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    [Graphical view]
    PfamiPF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004983. MenD. 1 hit.
    SUPFAMiSSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00173. menD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    E4W8B1-1 [UniParc]FASTAAdd to Basket

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    MNPSTAQATA VVDELIRGGV REVVLCPGSR NAPLAFALQA ADAAGRLRLH    50
    LRIDERTAGF LAIGLAVASG QPVPVVMTSG TAVANLGPAV LEANYARIPL 100
    IVLSANRPYE MLGTGANQTV EQFGLFGSQV RAAISLGLAE EAGDESQRGI 150
    RFDEQNSQWR SAVCRVLAAA RGTRSGNAGP VHFDIPLREP LVPDVRPRGL 200
    VPEGRPGGAA WTTTVHATLD VPMDLDITAD TVVVSGHGSA YRPELAGLPT 250
    VAEPTAPLHG IPLHPLALPQ LKPRQAIITG RPTLHRPVSK LLADPSVAVY 300
    ALTTGPRWPD VSGNVLATGT RAVVTGEPDP AWIARCRHLS EHADKAVRSQ 350
    LDAHPTATGL HVAAAVMEAL SDGDQLLLGA SNPVRDAALV SYPRPGVKVL 400
    SNRGVAGIDG TVSTAVGAAL AYESEGRTVA LLGDLTFLHD ASGLLIGTGE 450
    PRPTDLTIVV ANDDGGGIFE LLEQGDPQYA GVFERVFGTP HGMDLAALCA 500
    AYRVPHRRVD LAELTAVLTA PGDGIRVLEV TTERSGLREL HAAVRAQL 548
    Length:548
    Mass (Da):57,229
    Last modified:February 8, 2011 - v1
    Checksum:i864CFE51D4C22D8B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN563149 Genomic DNA. Translation: CBH49686.1.
    RefSeqiYP_004008365.1. NC_014659.1.

    Genome annotation databases

    EnsemblBacteriaiCBH49686; CBH49686; REQ_36990.
    GeneIDi9965964.
    KEGGireq:REQ_36990.
    PATRICi42664885. VBIRhoEqu141084_3702.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN563149 Genomic DNA. Translation: CBH49686.1 .
    RefSeqi YP_004008365.1. NC_014659.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBH49686 ; CBH49686 ; REQ_36990 .
    GeneIDi 9965964.
    KEGGi req:REQ_36990.
    PATRICi 42664885. VBIRhoEqu141084_3702.

    Phylogenomic databases

    HOGENOMi HOG000218359.
    KOi K02551.
    OMAi WRSAVCR.

    Enzyme and pathway databases

    UniPathwayi UPA00079 ; UER00164 .
    BioCyci REQU685727:GHKP-3581-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.970. 2 hits.
    HAMAPi MF_01659. MenD.
    InterProi IPR004433. MenaQ_synth_MenD.
    IPR029061. THDP-binding.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    [Graphical view ]
    Pfami PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004983. MenD. 1 hit.
    SUPFAMi SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00173. menD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 103SImported.

    Entry informationi

    Entry nameiE4W8B1_RHOE1
    AccessioniPrimary (citable) accession number: E4W8B1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 8, 2011
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3