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E4W8B1

- E4W8B1_RHOE1

UniProt

E4W8B1 - E4W8B1_RHOE1

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Protein
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Gene
menD, REQ_36990
Organism
Rhodococcus equi (strain 103S) (Corynebacterium equi)
Status
Unreviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotationSAAS annotations

Cofactori

Binds 1 thiamine pyrophosphate per subunit By similarity.UniRule annotationSAAS annotations
Magnesium or manganese By similarity.UniRule annotationSAAS annotations

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotations

Keywords - Biological processi

Menaquinone biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotations, ManganeseUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Thiamine pyrophosphateUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciREQU685727:GHKP-3581-MONOMER.
UniPathwayiUPA00079; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene namesi
Name:menDUniRule annotationImported
Ordered Locus Names:REQ_36990Imported
OrganismiRhodococcus equi (strain 103S) (Corynebacterium equi)Imported
Taxonomic identifieri685727 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000006892: Chromosome

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotationSAAS annotations

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218359.
KOiK02551.
OMAiWRSAVCR.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

E4W8B1-1 [UniParc]FASTAAdd to Basket

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MNPSTAQATA VVDELIRGGV REVVLCPGSR NAPLAFALQA ADAAGRLRLH    50
LRIDERTAGF LAIGLAVASG QPVPVVMTSG TAVANLGPAV LEANYARIPL 100
IVLSANRPYE MLGTGANQTV EQFGLFGSQV RAAISLGLAE EAGDESQRGI 150
RFDEQNSQWR SAVCRVLAAA RGTRSGNAGP VHFDIPLREP LVPDVRPRGL 200
VPEGRPGGAA WTTTVHATLD VPMDLDITAD TVVVSGHGSA YRPELAGLPT 250
VAEPTAPLHG IPLHPLALPQ LKPRQAIITG RPTLHRPVSK LLADPSVAVY 300
ALTTGPRWPD VSGNVLATGT RAVVTGEPDP AWIARCRHLS EHADKAVRSQ 350
LDAHPTATGL HVAAAVMEAL SDGDQLLLGA SNPVRDAALV SYPRPGVKVL 400
SNRGVAGIDG TVSTAVGAAL AYESEGRTVA LLGDLTFLHD ASGLLIGTGE 450
PRPTDLTIVV ANDDGGGIFE LLEQGDPQYA GVFERVFGTP HGMDLAALCA 500
AYRVPHRRVD LAELTAVLTA PGDGIRVLEV TTERSGLREL HAAVRAQL 548
Length:548
Mass (Da):57,229
Last modified:February 8, 2011 - v1
Checksum:i864CFE51D4C22D8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN563149 Genomic DNA. Translation: CBH49686.1.
RefSeqiYP_004008365.1. NC_014659.1.

Genome annotation databases

EnsemblBacteriaiCBH49686; CBH49686; REQ_36990.
GeneIDi9965964.
KEGGireq:REQ_36990.
PATRICi42664885. VBIRhoEqu141084_3702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN563149 Genomic DNA. Translation: CBH49686.1 .
RefSeqi YP_004008365.1. NC_014659.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBH49686 ; CBH49686 ; REQ_36990 .
GeneIDi 9965964.
KEGGi req:REQ_36990.
PATRICi 42664885. VBIRhoEqu141084_3702.

Phylogenomic databases

HOGENOMi HOG000218359.
KOi K02551.
OMAi WRSAVCR.

Enzyme and pathway databases

UniPathwayi UPA00079 ; UER00164 .
BioCyci REQU685727:GHKP-3581-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 103S.

Entry informationi

Entry nameiE4W8B1_RHOE1
AccessioniPrimary (citable) accession number: E4W8B1
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: June 11, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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