ID   PDSA_ARTGP              Reviewed;         735 AA.
AC   E4V6I8;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   24-JAN-2024, entry version 53.
DE   RecName: Full=Protostadienol synthase A;
DE            EC=5.4.99.32;
GN   Name=PDSA; ORFNames=MGYG_08628;
OS   Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum
OS   gypseum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Arthrodermataceae; Nannizzia.
OX   NCBI_TaxID=535722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4604 / CBS 118893;
RX   PubMed=22951933; DOI=10.1128/mbio.00259-12;
RA   Martinez D.A., Oliver B.G., Graeser Y., Goldberg J.M., Li W.,
RA   Martinez-Rossi N.M., Monod M., Shelest E., Barton R.C., Birch E.,
RA   Brakhage A.A., Chen Z., Gurr S.J., Heiman D., Heitman J., Kosti I.,
RA   Rossi A., Saif S., Samalova M., Saunders C.W., Shea T., Summerbell R.C.,
RA   Xu J., Young S., Zeng Q., Birren B.W., Cuomo C.A., White T.C.;
RT   "Comparative genome analysis of Trichophyton rubrum and related
RT   dermatophytes reveals candidate genes involved in infection.";
RL   MBio 3:E259-E259(2012).
CC   -!- FUNCTION: Protostadienol synthase which cyclizes (3S)-oxidosqualene to
CC       (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the
CC       biosynthetic precursor of helvolic acid, a secondary metabolite which
CC       promotes virulence. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = (17Z)-protosta-17(20),24-dien-3beta-
CC         ol; Xref=Rhea:RHEA:30987, ChEBI:CHEBI:15441, ChEBI:CHEBI:62457;
CC         EC=5.4.99.32;
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; DS989831; EFQ96704.1; -; Genomic_DNA.
DR   RefSeq; XP_003169081.1; XM_003169033.1.
DR   AlphaFoldDB; E4V6I8; -.
DR   SMR; E4V6I8; -.
DR   STRING; 535722.E4V6I8; -.
DR   GeneID; 10024264; -.
DR   VEuPathDB; FungiDB:MGYG_08628; -.
DR   eggNOG; KOG0497; Eukaryota.
DR   HOGENOM; CLU_009074_2_1_1; -.
DR   InParanoid; E4V6I8; -.
DR   OMA; QMEIKRY; -.
DR   OrthoDB; 2712461at2759; -.
DR   Proteomes; UP000002669; Unassembled WGS sequence.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   Gene3D; 1.50.10.20; -; 2.
DR   Gene3D; 6.20.120.20; -; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   NCBIfam; TIGR01787; squalene_cyclas; 1.
DR   PANTHER; PTHR11764:SF20; LANOSTEROL SYNTHASE; 1.
DR   PANTHER; PTHR11764; TERPENE CYCLASE/MUTASE FAMILY MEMBER; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2.
PE   3: Inferred from homology;
KW   Isomerase; Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW   Repeat; Steroid biosynthesis; Virulence.
FT   CHAIN           1..735
FT                   /note="Protostadienol synthase A"
FT                   /id="PRO_0000415498"
FT   REPEAT          129..170
FT                   /note="PFTB 1"
FT   REPEAT          487..528
FT                   /note="PFTB 2"
FT   REPEAT          564..604
FT                   /note="PFTB 3"
FT   REPEAT          613..660
FT                   /note="PFTB 4"
FT   ACT_SITE        460
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   735 AA;  82905 MW;  263F8DEB2E3AA290 CRC64;
     MPVADIEMVT GSAGHVDSKA ASEYGTDLRR WRLKVHEGRH MWEYVDEDVA QTQQQTFAEN
     YWLGQEYELP KMSTPIFAQD ALDNGWAFFK RLQTQDGHWG CHDDGPLFVT SGIVISSYIC
     GITLPDAMKN EMIRYLLNFV NEDGGWGLWI NSPSTVFGTT MNYTMLRILG VPSTHPALLD
     ARDTLLKMGS ARALPTWGKF WMCALGAYEW DGMIPLAPEP LLAPGFLPLN PGNWWVHTRN
     VFVSMSYLFG HRFSAPMTPL IQELREELYD MPYHKIDWFA QQTNISDYDR LHPPTMLQKG
     LANALCYYEY VKVPYLRRKA LDEALFQVEM EVHNTSYLCI APVSFASNML VMFHAHGANS
     HWVKGMADRI IDPMWMCREG MAASGTNGTS VWDTALTVQA ALDGGLAQRP ENHNTMLEAL
     KFIEVSQITE NPLGVSQGYR QPTKGAWPFS TRDQAYAVSD TTAVTVRAVI QLQALKSMPK
     LVSDERLAEA VDLIIGMENK CDGYSAFEPL RGPKALELLN ITELYDNVMT ESLYPECTSS
     VLLCLDTFTK AYPHHRPVEI QSIMARCARY LIKAQFPCGG WLASWGVCFT YATMFALQGL
     ETVGLRESNS ETCRNACSFL LQYQNDDGGW GEDLISIREK RYIQDPAGSQ VTCTAYALMG
     LISAHCSNRD ALRRGIRWLM RAQQATGEWL PGSLEGIFAC PGGMRYPNYK FHFTLSAIGR
     YIERYGDEAL YLKEQ
//