E4V6I8 (PDSA_ARTGP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protostadienol synthase A
EC=5.4.99.32

Gene names

Name:	PDSA
ORF Names:	MGYG_08628

Organism

Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum) [Complete proteome]

Taxonomic identifier

535722 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › Arthrodermataceae › Arthroderma › Arthroderma gypseum

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence By similarity.
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 4 PFTB repeats.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Virulence
Domain	Repeat
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	intramolecular transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Protostadienol synthase A		PRO_0000415498
Regions
	Repeat	129 – 170	42	PFTB 1
	Repeat	487 – 528	42	PFTB 2
	Repeat	564 – 604	41	PFTB 3
	Repeat	613 – 660	48	PFTB 4
Sites
	Active site	237	1	Proton acceptor By similarity
	Active site	460	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	E4V6I8 [UniParc]. Last modified February 8, 2011. Version 1. Checksum: 263F8DEB2E3AA290	FASTA	735	82,905
	10 20 30 40 50 60 MPVADIEMVT GSAGHVDSKA ASEYGTDLRR WRLKVHEGRH MWEYVDEDVA QTQQQTFAEN 70 80 90 100 110 120 YWLGQEYELP KMSTPIFAQD ALDNGWAFFK RLQTQDGHWG CHDDGPLFVT SGIVISSYIC 130 140 150 160 170 180 GITLPDAMKN EMIRYLLNFV NEDGGWGLWI NSPSTVFGTT MNYTMLRILG VPSTHPALLD 190 200 210 220 230 240 ARDTLLKMGS ARALPTWGKF WMCALGAYEW DGMIPLAPEP LLAPGFLPLN PGNWWVHTRN 250 260 270 280 290 300 VFVSMSYLFG HRFSAPMTPL IQELREELYD MPYHKIDWFA QQTNISDYDR LHPPTMLQKG 310 320 330 340 350 360 LANALCYYEY VKVPYLRRKA LDEALFQVEM EVHNTSYLCI APVSFASNML VMFHAHGANS 370 380 390 400 410 420 HWVKGMADRI IDPMWMCREG MAASGTNGTS VWDTALTVQA ALDGGLAQRP ENHNTMLEAL 430 440 450 460 470 480 KFIEVSQITE NPLGVSQGYR QPTKGAWPFS TRDQAYAVSD TTAVTVRAVI QLQALKSMPK 490 500 510 520 530 540 LVSDERLAEA VDLIIGMENK CDGYSAFEPL RGPKALELLN ITELYDNVMT ESLYPECTSS 550 560 570 580 590 600 VLLCLDTFTK AYPHHRPVEI QSIMARCARY LIKAQFPCGG WLASWGVCFT YATMFALQGL 610 620 630 640 650 660 ETVGLRESNS ETCRNACSFL LQYQNDDGGW GEDLISIREK RYIQDPAGSQ VTCTAYALMG 670 680 690 700 710 720 LISAHCSNRD ALRRGIRWLM RAQQATGEWL PGSLEGIFAC PGGMRYPNYK FHFTLSAIGR 730 YIERYGDEAL YLKEQ

References

[1]

"Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection." Martinez D.A., Oliver B.G., Graeser Y., Goldberg J.M., Li W., Martinez-Rossi N.M., Monod M., Shelest E., Barton R.C., Birch E., Brakhage A.A., Chen Z., Gurr S.J., Heiman D., Heitman J., Kosti I., Rossi A., Saif S. , Samalova M., Saunders C.W., Shea T., Summerbell R.C., Xu J., Young S., Zeng Q., Birren B.W., Cuomo C.A., White T.C. MBio 3:E259-E259(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4604 / CBS 118893.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS989831 Genomic DNA. Translation: EFQ96704.1.
RefSeq	XP_003169081.1. XM_003169033.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10024264.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDSA_ARTGP

Accession

Primary (citable) accession number: E4V6I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	February 8, 2011
Last modified:	March 6, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program