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Protein

Protostadienol synthase A

Gene

PDSA

Organism
Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence.By similarity

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei237 – 2371Proton acceptorBy similarity
Active sitei460 – 4601Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Protostadienol synthase A (EC:5.4.99.32)
Gene namesi
Name:PDSA
ORF Names:MGYG_08628
OrganismiArthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum)
Taxonomic identifieri535722 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesArthrodermataceaeMicrosporum
ProteomesiUP000002669 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Protostadienol synthase APRO_0000415498Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi535722.XP_003169081.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati129 – 17042PFTB 1Add
BLAST
Repeati487 – 52842PFTB 2Add
BLAST
Repeati564 – 60441PFTB 3Add
BLAST
Repeati613 – 66048PFTB 4Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiE4V6I8.
OrthoDBiEOG70610B.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.

Sequencei

Sequence statusi: Complete.

E4V6I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVADIEMVT GSAGHVDSKA ASEYGTDLRR WRLKVHEGRH MWEYVDEDVA
60 70 80 90 100
QTQQQTFAEN YWLGQEYELP KMSTPIFAQD ALDNGWAFFK RLQTQDGHWG
110 120 130 140 150
CHDDGPLFVT SGIVISSYIC GITLPDAMKN EMIRYLLNFV NEDGGWGLWI
160 170 180 190 200
NSPSTVFGTT MNYTMLRILG VPSTHPALLD ARDTLLKMGS ARALPTWGKF
210 220 230 240 250
WMCALGAYEW DGMIPLAPEP LLAPGFLPLN PGNWWVHTRN VFVSMSYLFG
260 270 280 290 300
HRFSAPMTPL IQELREELYD MPYHKIDWFA QQTNISDYDR LHPPTMLQKG
310 320 330 340 350
LANALCYYEY VKVPYLRRKA LDEALFQVEM EVHNTSYLCI APVSFASNML
360 370 380 390 400
VMFHAHGANS HWVKGMADRI IDPMWMCREG MAASGTNGTS VWDTALTVQA
410 420 430 440 450
ALDGGLAQRP ENHNTMLEAL KFIEVSQITE NPLGVSQGYR QPTKGAWPFS
460 470 480 490 500
TRDQAYAVSD TTAVTVRAVI QLQALKSMPK LVSDERLAEA VDLIIGMENK
510 520 530 540 550
CDGYSAFEPL RGPKALELLN ITELYDNVMT ESLYPECTSS VLLCLDTFTK
560 570 580 590 600
AYPHHRPVEI QSIMARCARY LIKAQFPCGG WLASWGVCFT YATMFALQGL
610 620 630 640 650
ETVGLRESNS ETCRNACSFL LQYQNDDGGW GEDLISIREK RYIQDPAGSQ
660 670 680 690 700
VTCTAYALMG LISAHCSNRD ALRRGIRWLM RAQQATGEWL PGSLEGIFAC
710 720 730
PGGMRYPNYK FHFTLSAIGR YIERYGDEAL YLKEQ
Length:735
Mass (Da):82,905
Last modified:February 8, 2011 - v1
Checksum:i263F8DEB2E3AA290
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS989831 Genomic DNA. Translation: EFQ96704.1.
RefSeqiXP_003169081.1. XM_003169033.1.

Genome annotation databases

GeneIDi10024264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS989831 Genomic DNA. Translation: EFQ96704.1.
RefSeqiXP_003169081.1. XM_003169033.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi535722.XP_003169081.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi10024264.

Phylogenomic databases

InParanoidiE4V6I8.
OrthoDBiEOG70610B.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4604 / CBS 118893.

Entry informationi

Entry nameiPDSA_ARTGP
AccessioniPrimary (citable) accession number: E4V6I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: February 8, 2011
Last modified: June 24, 2015
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.