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E4V6I8 (PDSA_ARTGP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protostadienol synthase A

EC=5.4.99.32
Gene names
Name:PDSA
ORF Names:MGYG_08628
OrganismArthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum) [Complete proteome]
Taxonomic identifier535722 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesArthrodermataceaeArthroderma

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence By similarity.

Catalytic activity

(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 4 PFTB repeats.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
Steroid biosynthesis
Virulence
   DomainRepeat
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

steroid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionintramolecular transferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Protostadienol synthase A
PRO_0000415498

Regions

Repeat129 – 17042PFTB 1
Repeat487 – 52842PFTB 2
Repeat564 – 60441PFTB 3
Repeat613 – 66048PFTB 4

Sites

Active site2371Proton acceptor By similarity
Active site4601Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
E4V6I8 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: 263F8DEB2E3AA290

FASTA73582,905
        10         20         30         40         50         60 
MPVADIEMVT GSAGHVDSKA ASEYGTDLRR WRLKVHEGRH MWEYVDEDVA QTQQQTFAEN 

        70         80         90        100        110        120 
YWLGQEYELP KMSTPIFAQD ALDNGWAFFK RLQTQDGHWG CHDDGPLFVT SGIVISSYIC 

       130        140        150        160        170        180 
GITLPDAMKN EMIRYLLNFV NEDGGWGLWI NSPSTVFGTT MNYTMLRILG VPSTHPALLD 

       190        200        210        220        230        240 
ARDTLLKMGS ARALPTWGKF WMCALGAYEW DGMIPLAPEP LLAPGFLPLN PGNWWVHTRN 

       250        260        270        280        290        300 
VFVSMSYLFG HRFSAPMTPL IQELREELYD MPYHKIDWFA QQTNISDYDR LHPPTMLQKG 

       310        320        330        340        350        360 
LANALCYYEY VKVPYLRRKA LDEALFQVEM EVHNTSYLCI APVSFASNML VMFHAHGANS 

       370        380        390        400        410        420 
HWVKGMADRI IDPMWMCREG MAASGTNGTS VWDTALTVQA ALDGGLAQRP ENHNTMLEAL 

       430        440        450        460        470        480 
KFIEVSQITE NPLGVSQGYR QPTKGAWPFS TRDQAYAVSD TTAVTVRAVI QLQALKSMPK 

       490        500        510        520        530        540 
LVSDERLAEA VDLIIGMENK CDGYSAFEPL RGPKALELLN ITELYDNVMT ESLYPECTSS 

       550        560        570        580        590        600 
VLLCLDTFTK AYPHHRPVEI QSIMARCARY LIKAQFPCGG WLASWGVCFT YATMFALQGL 

       610        620        630        640        650        660 
ETVGLRESNS ETCRNACSFL LQYQNDDGGW GEDLISIREK RYIQDPAGSQ VTCTAYALMG 

       670        680        690        700        710        720 
LISAHCSNRD ALRRGIRWLM RAQQATGEWL PGSLEGIFAC PGGMRYPNYK FHFTLSAIGR 

       730 
YIERYGDEAL YLKEQ 

« Hide

References

[1]"Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection."
Martinez D.A., Oliver B.G., Graeser Y., Goldberg J.M., Li W., Martinez-Rossi N.M., Monod M., Shelest E., Barton R.C., Birch E., Brakhage A.A., Chen Z., Gurr S.J., Heiman D., Heitman J., Kosti I., Rossi A., Saif S. expand/collapse author list , Samalova M., Saunders C.W., Shea T., Summerbell R.C., Xu J., Young S., Zeng Q., Birren B.W., Cuomo C.A., White T.C.
MBio 3:E259-E259(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC MYA-4604 / CBS 118893.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS989831 Genomic DNA. Translation: EFQ96704.1.
RefSeqXP_003169081.1. XM_003169033.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID10024264.

Phylogenomic databases

OrthoDBEOG70610B.

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01787. squalene_cyclas. 1 hit.
ProtoNetSearch...

Entry information

Entry namePDSA_ARTGP
AccessionPrimary (citable) accession number: E4V6I8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families