E4UPP6 (LCPS_ARTGP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

1. Lycopene beta-cyclase
   EC=5.5.1.19
   Alternative name(s):
   Lycopene cyclase
2. Phytoene synthase
   EC=2.5.1.32

Gene names

ORF Names:

MGYG_02096

Organism

Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum) [Complete proteome]

Taxonomic identifier

535722 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › Arthrodermataceae › Arthroderma › Arthroderma gypseum

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase) By similarity.
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC intramolecular lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000409231
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	130 – 150	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Transmembrane	227 – 247	21	Helical; Potential
	Region	1 – 249	249	Lycopene beta-cyclase
	Region	256 – 594	339	Phytoene synthase

Sequences

	Sequence		Length	Mass (Da)
	E4UPP6 [UniParc]. Last modified February 8, 2011. Version 1. Checksum: 481889862E516E29	FASTA	594	67,784
	10 20 30 40 50 60 MGLDYLMVHV KYNLPPALLL TILYKPFFTR LEVHKIVLLC TIAVVWTIPW DSYLIRTRIW 70 80 90 100 110 120 SYPADSVLGQ TLFQIPLEEV FFFIIQTYNT SLLYIIFNKR LVLPSYLSGP TKPLAQGLFG 130 140 150 160 170 180 PITHRTQRDL GTLFFTGILI LGISFIYIGG EYMYLGLILS WVSPILVMQW VLMYRFLLAL 190 200 210 220 230 240 PPASVWVPIA LPTLYLWVVD TLALRRGTWV IESGTKVDIQ LWEGLEIEEA LFFLVTNVMV 250 260 270 280 290 300 VFGIAAMHNA AALFEYKAFI STTAMGDTPS IYQLITLFLT SSRLYDTNVL QEMSQAVTLL 310 320 330 340 350 360 KQKSQSMYLG SAMFEGQLRL DLIALYSFCR KADDLIDDAP DRETAKYWIE QCEKALELRF 370 380 390 400 410 420 KLKETALDDT EAYQLLTKSI PQPLHAAAHL LPASRLPKEP LSCLLQGFEI DLKFDFEKGS 430 440 450 460 470 480 FPIATEHDLE VYAYHVAGTV ASLLLELVFR HHPVSISEAE RLRVISAGEV MGRALQYTNI 490 500 510 520 530 540 ARDITRDAEI GRVYIPSSWL AEEGLTPSMV ISHPRNSKLI PLRRRILEKA DKCYCETQEA 550 560 570 580 590 ISKLPSNVQG PVRATVTAYM EIGQVIRENE TKIWNGKLKV SRWRRFKRAW LAML

References

[1]

"Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection." Martinez D.A., Oliver B.G., Graeser Y., Goldberg J.M., Li W., Martinez-Rossi N.M., Monod M., Shelest E., Barton R.C., Birch E., Brakhage A.A., Chen Z., Gurr S.J., Heiman D., Heitman J., Kosti I., Rossi A., Saif S. , Samalova M., Saunders C.W., Shea T., Summerbell R.C., Xu J., Young S., Zeng Q., Birren B.W., Cuomo C.A., White T.C. MBio 3:E259-E259(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4604 / CBS 118893.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS989823 Genomic DNA. Translation: EFQ99083.1.
RefSeq	XP_003174566.1. XM_003174518.1.
3D structure databases
ProteinModelPortal	E4UPP6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10029862.
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_ARTGP

Accession

Primary (citable) accession number: E4UPP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	February 8, 2011
Last modified:	March 6, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program