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E4TIZ2

- E4TIZ2_CALNY

UniProt

E4TIZ2 - E4TIZ2_CALNY

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Calditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (08 Feb 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei223 – 2231SubstrateUniRule annotation
    Binding sitei244 – 2441Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi102 – 1054ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCNIT768670:GHD1-1240-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Calni_1216Imported
    OrganismiCalditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1)Imported
    Taxonomic identifieri768670 [NCBI]
    Taxonomic lineageiBacteriaDeferribacteresDeferribacteralesDeferribacteraceae
    ProteomesiUP000007039: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929 PotentialImportedAdd
    BLAST
    Chaini30 – 320291 PotentialPRO_5000662451Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
    Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
    Regioni250 – 2534Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Keywords - Domaini

    SignalImported

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiMELREGH.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    E4TIZ2-1 [UniParc]FASTAAdd to Basket

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    MKRIAVMTSG GDCPGMNAAI RSVVRTAIAH NLEPFGIEQG YKGLIEGKII    50
    PMSNKSVANI IQRGGTILKS ARSMEFKTDE GQRKAVNNLA ELGIEGLIII 100
    GGDGSLTGAK ILSERYGIKT IGIPGSIDND LCCTDMSIGV DTALNTIVRA 150
    VDSINDTASS HDRTFLIEVM GRNCGYLALV SAIATGAEAV LIPEVQYDIE 200
    KIIAKIKKRY EEGKSRSIII IAEGVGSAMD FGKVFGMIGG FDTRVTVLGH 250
    LQRGGSPTVF DRILATRMGT AAVEALLDGV TASMTALQKT KIELVPFKDI 300
    IGLKKPLDHK LIEIAEVLSR 320
    Length:320
    Mass (Da):34,104
    Last modified:February 8, 2011 - v1
    Checksum:i12474B17187A3047
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002347 Genomic DNA. Translation: ADR19124.1.
    RefSeqiYP_004051287.1. NC_014758.1.

    Genome annotation databases

    EnsemblBacteriaiADR19124; ADR19124; Calni_1216.
    GeneIDi10009238.
    KEGGicni:Calni_1216.
    PATRICi45190187. VBICalNit163602_1297.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002347 Genomic DNA. Translation: ADR19124.1 .
    RefSeqi YP_004051287.1. NC_014758.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADR19124 ; ADR19124 ; Calni_1216 .
    GeneIDi 10009238.
    KEGGi cni:Calni_1216.
    PATRICi 45190187. VBICalNit163602_1297.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi MELREGH.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci CNIT768670:GHD1-1240-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 19672.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 19672 / NBRC 101217 / Yu37-1Imported.

    Entry informationi

    Entry nameiE4TIZ2_CALNY
    AccessioniPrimary (citable) accession number: E4TIZ2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 8, 2011
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3