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E4TIZ2

- E4TIZ2_CALNY

UniProt

E4TIZ2 - E4TIZ2_CALNY

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, Calni_1216
Organism
Calditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi103 – 1031Magnesium; catalytic By similarityUniRule annotation
Active sitei128 – 1281Proton acceptor By similarityUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei223 – 2231Substrate By similarityUniRule annotation
Binding sitei244 – 2441Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarityUniRule annotation
Nucleotide bindingi102 – 1054ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciCNIT768670:GHD1-1240-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Calni_1216Imported
OrganismiCalditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1)Imported
Taxonomic identifieri768670 [NCBI]
Taxonomic lineageiBacteriaDeferribacteresDeferribacteralesDeferribacteraceae
ProteomesiUP000007039: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 PredictedImportedAdd
BLAST
Chaini30 – 320291 PredictedPRO_5000662451Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni126 – 1283Substrate binding By similarityUniRule annotation
Regioni170 – 1723Substrate binding By similarityUniRule annotation
Regioni186 – 1883Allosteric activator ADP binding By similarityUniRule annotation
Regioni214 – 2163Allosteric activator ADP binding By similarityUniRule annotation
Regioni250 – 2534Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Keywords - Domaini

SignalImported

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiMELREGH.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E4TIZ2-1 [UniParc]FASTAAdd to Basket

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MKRIAVMTSG GDCPGMNAAI RSVVRTAIAH NLEPFGIEQG YKGLIEGKII    50
PMSNKSVANI IQRGGTILKS ARSMEFKTDE GQRKAVNNLA ELGIEGLIII 100
GGDGSLTGAK ILSERYGIKT IGIPGSIDND LCCTDMSIGV DTALNTIVRA 150
VDSINDTASS HDRTFLIEVM GRNCGYLALV SAIATGAEAV LIPEVQYDIE 200
KIIAKIKKRY EEGKSRSIII IAEGVGSAMD FGKVFGMIGG FDTRVTVLGH 250
LQRGGSPTVF DRILATRMGT AAVEALLDGV TASMTALQKT KIELVPFKDI 300
IGLKKPLDHK LIEIAEVLSR 320
Length:320
Mass (Da):34,104
Last modified:February 8, 2011 - v1
Checksum:i12474B17187A3047
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002347 Genomic DNA. Translation: ADR19124.1.
RefSeqiYP_004051287.1. NC_014758.1.

Genome annotation databases

EnsemblBacteriaiADR19124; ADR19124; Calni_1216.
GeneIDi10009238.
KEGGicni:Calni_1216.
PATRICi45190187. VBICalNit163602_1297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002347 Genomic DNA. Translation: ADR19124.1 .
RefSeqi YP_004051287.1. NC_014758.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADR19124 ; ADR19124 ; Calni_1216 .
GeneIDi 10009238.
KEGGi cni:Calni_1216.
PATRICi 45190187. VBICalNit163602_1297.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi MELREGH.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci CNIT768670:GHD1-1240-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 19672.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 19672 / NBRC 101217 / Yu37-1.

Entry informationi

Entry nameiE4TIZ2_CALNY
AccessioniPrimary (citable) accession number: E4TIZ2
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: July 9, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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