ID   E4TDW4_RIEAD            Unreviewed;       279 AA.
AC   E4TDW4;
DT   08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT   08-FEB-2011, sequence version 1.
DT   24-JAN-2024, entry version 56.
DE   RecName: Full=Type-2 restriction enzyme {ECO:0000256|PIRNR:PIRNR016080};
DE            EC=3.1.21.4 {ECO:0000256|PIRNR:PIRNR016080};
GN   ORFNames=RA0C_0017 {ECO:0000313|EMBL:AFD55042.1};
OS   Riemerella anatipestifer (strain ATCC 11845 / DSM 15868 / JCM 9532 /
OS   NCTC 11014).
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC   Riemerella.
OX   NCBI_TaxID=693978 {ECO:0000313|EMBL:AFD55042.1, ECO:0000313|Proteomes:UP000010093};
RN   [1] {ECO:0000313|EMBL:AFD55042.1, ECO:0000313|Proteomes:UP000010093}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15868 {ECO:0000313|Proteomes:UP000010093};
RX   PubMed=22628503; DOI=10.1128/JB.00366-12;
RA   Wang X., Zhu D., Wang M., Cheng A., Jia R., Zhou Y., Chen Z., Luo Q.,
RA   Liu F., Wang Y., Chen X.Y.;
RT   "Complete genome sequence of Riemerella anatipestifer reference strain.";
RL   J. Bacteriol. 194:3270-3271(2012).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded unmethylated sequence 5'-GATC-3'.
CC       {ECO:0000256|PIRNR:PIRNR016080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000256|PIRNR:PIRNR016080};
CC   -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC       family. {ECO:0000256|PIRNR:PIRNR016080}.
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DR   EMBL; CP003388; AFD55042.1; -; Genomic_DNA.
DR   RefSeq; WP_004920743.1; NC_017045.1.
DR   AlphaFoldDB; E4TDW4; -.
DR   REBASE; 46596; Ran15868CORF16P.
DR   REBASE; 46862; Ran15868ORF1817P.
DR   GeneID; 66849472; -.
DR   KEGG; rai:RA0C_0017; -.
DR   KEGG; ran:Riean_1818; -.
DR   PATRIC; fig|693978.17.peg.18; -.
DR   HOGENOM; CLU_089327_0_0_10; -.
DR   Proteomes; UP000010093; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-UniRule.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR   InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR   Pfam; PF04556; DpnII; 1.
DR   PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
DR   SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE   3: Inferred from homology;
KW   Endonuclease {ECO:0000256|PIRNR:PIRNR016080};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR016080};
KW   Nuclease {ECO:0000256|PIRNR:PIRNR016080};
KW   Restriction system {ECO:0000256|PIRNR:PIRNR016080}.
FT   DOMAIN          4..269
FT                   /note="Restriction endonuclease type II DpnII-like"
FT                   /evidence="ECO:0000259|Pfam:PF04556"
SQ   SEQUENCE   279 AA;  32137 MW;  D1D54513F1E774BE CRC64;
     MSEQFKIFLS QLSETNATLD YFADFKKIRN NVNKIAIKLN QLNYLIGKEN LKEAVIELYE
     ENSKVFEVLD ILIAIRKNKN AKTFNNKGEI VLLDTYFTSP ELILEYIEET GLAEVFRNKD
     VTNLVDYVFG IEVGLDTNAR KNRGGDNMSK AVSLIFDKAG VFYKKEVNNT IFPEIISLGA
     DVKRFDFVIK TKKKTYLIET NYYNSGGSKL NETARSYSDV APKINLYEGY EFVWITDGQG
     WFSAKNKLEE AYNIIPSLYN LITLEDFIKK IQEETVIEF
//