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E4SRC3 (E4SRC3_STRTN) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:STND_1517 EMBL ADQ63553.1
OrganismStreptococcus thermophilus (strain ND03) [Complete proteome] [HAMAP] EMBL ADQ63553.1
Taxonomic identifier767463 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain132 – 334203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding162 – 21756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2881Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3011Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3011Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3031Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
E4SRC3 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: EE74CE5234C53385

FASTA34838,514
        10         20         30         40         50         60 
MSKQTLILLY GGRSAEREVS VLSAESVMRA VDYNAFEVKT YFITQSGDFI KTQEFIETPG 

        70         80         90        100        110        120 
DDEKLMTNDT VEASQAIKPS DIYEEDAVVF PVLHGPMGED GSIQGFLETL KLPYVGTNVL 

       130        140        150        160        170        180 
SSSVAMDKIM TKRILEVAGV PQVAYTGYIE GEDLEAAVAE TLEKLTFPVF VKPANMGSSV 

       190        200        210        220        230        240 
GISKAENESE LRSAIDLALK YDSRILIEQG VVAREIEVGI LGNTTVKTTD PGEVVKDVAF 

       250        260        270        280        290        300 
YDYQAKYIDN KITMDIPARV PVEVMTQMRA YAAKAFRALG GCGLARCDFF LTEDGAIYLN 

       310        320        330        340 
ELNTMPGFTQ WSMYPLLWEN MGLSYSDLIK ELVVLGQEMF DKRESHLI 

« Hide

References

[1]"Complete genome sequence of Streptococcus thermophilus strain ND03."
Sun Z., Chen X., Wang J., Zhao W., Shao Y., Wu L., Zhou Z., Sun T., Wang L., Meng H., Zhang H., Chen W.
J. Bacteriol. 193:793-794(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ND03 EMBL ADQ63553.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002340 Genomic DNA. Translation: ADQ63553.1.
RefSeqYP_006003057.1. NC_017563.1.

3D structure databases

ProteinModelPortalE4SRC3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADQ63553; ADQ63553; STND_1517.
GeneID12591676.
KEGGstn:STND_1517.
PATRIC43256595. VBIStrThe160185_1609.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycSTHE767463:GLLS-1576-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE4SRC3_STRTN
AccessionPrimary (citable) accession number: E4SRC3
Entry history
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: February 19, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)