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E4SPH7

- E4SPH7_STRTN

UniProt

E4SPH7 - E4SPH7_STRTN

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Streptococcus thermophilus (strain ND03)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPUniRule annotation
Nucleotide bindingi102 – 1054ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE767463:GLLS-1189-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:STND_1133Imported
OrganismiStreptococcus thermophilus (strain ND03)Imported
Taxonomic identifieri767463 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000008716: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliE4SPH7.
SMRiE4SPH7. Positions 1-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1273Substrate bindingUniRule annotation
Regioni169 – 1713Substrate bindingUniRule annotation
Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
Regioni251 – 2544Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

E4SPH7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRIAVLTSG GDAPGMNAAV RAVVLKAISE GIEVFGINRG YAGMVEGDIF
60 70 80 90 100
KLDAKRVENI LSRGGTFLQS ARYPEFAQLE GQLKGIEQLK KYGIEGVVVI
110 120 130 140 150
GGDGSYHGAM RLTEHGFPAV GLPGTIDNDI VGTDYTIGFD TAVATATEAL
160 170 180 190 200
DKIQDTAFSH GRTFVVEVMG RNAGDIALWA GIASGADQII VPEEEYDINE
210 220 230 240 250
VVRKVKEGYE SGEKSHHIIV LAEGVMGAEE FAAKMKEAGD TSDLRATNLG
260 270 280 290 300
HVIRGGSPTA RDRVLASWMG AHAVDLLKEG IGGVAVGIHN EQLVESPILG
310 320 330
TAEEGALFSL TEDGKIIVNN PHKARLDFAE LNRSLANLK
Length:339
Mass (Da):35,982
Last modified:February 8, 2011 - v1
Checksum:i492DF7076EC443DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002340 Genomic DNA. Translation: ADQ63175.1.
RefSeqiYP_006002679.1. NC_017563.1.

Genome annotation databases

EnsemblBacteriaiADQ63175; ADQ63175; STND_1133.
GeneIDi12591289.
KEGGistn:STND_1133.
PATRICi43255790. VBIStrThe160185_1211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002340 Genomic DNA. Translation: ADQ63175.1 .
RefSeqi YP_006002679.1. NC_017563.1.

3D structure databases

ProteinModelPortali E4SPH7.
SMRi E4SPH7. Positions 1-304.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADQ63175 ; ADQ63175 ; STND_1133 .
GeneIDi 12591289.
KEGGi stn:STND_1133.
PATRICi 43255790. VBIStrThe160185_1211.

Phylogenomic databases

KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci STHE767463:GLLS-1189-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Streptococcus thermophilus strain ND03."
    Sun Z., Chen X., Wang J., Zhao W., Shao Y., Wu L., Zhou Z., Sun T., Wang L., Meng H., Zhang H., Chen W.
    J. Bacteriol. 193:793-794(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ND03Imported.

Entry informationi

Entry nameiE4SPH7_STRTN
AccessioniPrimary (citable) accession number: E4SPH7
Entry historyi
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: October 29, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3