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E4SNN0 (E4SNN0_LACAR) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tRNA sulfurtransferase HAMAP-Rule MF_00021
EC=2.8.1.4 HAMAP-Rule MF_00021
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase HAMAP-Rule MF_00021
Thiamine biosynthesis protein ThiI HAMAP-Rule MF_00021
tRNA 4-thiouridine synthase HAMAP-Rule MF_00021
Gene names
Name:thiI HAMAP-Rule MF_00021
Ordered Locus Names:LA2_04135 EMBL ADQ58800.1
OrganismLactobacillus amylovorus (strain GRL 1112) [Complete proteome] [HAMAP]
Taxonomic identifier695560 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus ›

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021 SAAS SAAS003720

Catalytic activity

-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021 SAAS SAAS003720

[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. SAAS SAAS003720

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021 SAAS SAAS003720

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00021 SAAS SAAS003720.

Sequence similarities

Belongs to the ThiI family. HAMAP-Rule MF_00021

Contains 1 THUMP domain. HAMAP-Rule MF_00021 SAAS SAAS003720

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain60 – 165106THUMP By similarity HAMAP-Rule MF_00021
Nucleotide binding183 – 1842ATP By similarity HAMAP-Rule MF_00021
Nucleotide binding208 – 2092ATP By similarity HAMAP-Rule MF_00021

Sites

Binding site2651ATP By similarity HAMAP-Rule MF_00021
Binding site2871ATP; via amide nitrogen By similarity HAMAP-Rule MF_00021
Binding site2961ATP By similarity HAMAP-Rule MF_00021

Sequences

Sequence LengthMass (Da)Tools
E4SNN0 [UniParc].

Last modified February 8, 2011. Version 1.
Checksum: FDD6780262C0E093

FASTA40545,911
        10         20         30         40         50         60 
MEYTEVMVRY GELSTKGKNR KDFINRLATN VERVLKDFPQ IEFHPRHDRM HIILNGAPFT 

        70         80         90        100        110        120 
EVDKRLKKVF GIQTYSPAIK VPKTLEDIKK TSLELMNETF KPGMTFKVNT KRSDHKFEYD 

       130        140        150        160        170        180 
TNQLNNLIGD YLFDHIDHLK AEMKHPDLVL RIEVRQDAVY ISNQLLHGIG GMPVGTAGKA 

       190        200        210        220        230        240 
VMMLSGGIDS PVASYLAMKR GVDIEMVHFF SPPYTTEKAL AKAKELTGIL ANYAGKINFI 

       250        260        270        280        290        300 
AVPFAEIQET IKEKLPEGYL MTVQRRFMLQ LADRIRAKRG GLAIFNGESV GQVASQTLQS 

       310        320        330        340        350        360 
MVAINDVTTT PVIRPVATMD KTEIIKLAED IGTFDLSIQP FEDCCTIFAP PRPKTKPKLD 

       370        380        390        400 
KAREYEARLD VEGLIQRAMD GIEVMPIYPN EKFINDKIEE DQDLL

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References

[1]"Genome sequence of Lactobacillus amylovorus GRL1112."
Kant R., Paulin L., Alatalo E., de Vos W.M., Palva A.
J. Bacteriol. 193:789-790(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GRL 1112 EMBL ADQ58800.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002338 Genomic DNA. Translation: ADQ58800.1.
RefSeqYP_004031595.1. NC_014724.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADQ58800; ADQ58800; LA2_04135.
GeneID10022842.
KEGGlam:LA2_04135.
PATRIC43076659. VBILacAmy150400_0823.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000227470.
KOK03151.
OMAFGIQAFS.

Enzyme and pathway databases

BioCycLAMY695560:GI0Z-924-MONOMER.
UniPathwayUPA00060.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00021. ThiI.
InterProIPR014729. Rossmann-like_a/b/a_fold.
IPR003720. ThiI.
IPR020536. ThiI_C_dom.
IPR004114. THUMP.
[Graphical view]
PfamPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsTIGR00342. TIGR00342. 1 hit.
PROSITEPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE4SNN0_LACAR
AccessionPrimary (citable) accession number: E4SNN0
Entry history
Integrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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